Transcriptional variations in biomarkers of<i>Mytilus galloprovincialis</i>sampled from Central Adriatic coastal waters (Marche region, Italy)

Cocci, Paolo; Capriotti, Martina; Mosconi, Gilberto; Palermo, Francesco Alessandro

doi:10.1080/1354750x.2017.1315614

Cited by 5 publications

(1 citation statement)

References 104 publications

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“…In bivalves, the digestive gland plays a central role like that of the liver in vertebrates, in the metabolism and detoxification of xenobiotic agents (Cocci et al, 2017;Rodrigo and Costa, 2017). Cyp4, the target gene in this study, encodes the CYP4 enzyme which is mainly involved in xenobiotic metabolisms (Hardwick, 2008;Lewis and Ito, 2008).…”

Section: Relative Expression Levels Of Cytochrome P450 4 (Cyp4) Usingmentioning

confidence: 99%

18S rRNA, a potential reference gene in the qRT-PCR measurement of bisphenol A contamination in green mussels (Perna viridis) collected from the Gulf of Thailand

2019

ANRES

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Normalization of reference genes through the selection of appropriate housekeeping genes is important for the reliable quantification of mRNA analyzed using real-time polymerase chain reaction (qRT-PCR). The suitability of various housekeeping genes (HKGs), were valuated as internal references to verify the levels of the contaminant bisphenol A (BPA, 2,2-bis (4-hydroxyphenyl) propane) by determining the level of gene expression of cytochrome P450 4 (cyp4) within the digestive gland of green mussels (Perna viridis)-a species used for biomonitoring environmental levels. Additionally, the effect was explored of mussel gender and maturity status (juvenile or mature) on the stability of the expression of the reference genes. Three candidate HKGs were examined, namely 18S rRNA, 28S rRNA and β-actin. The expression stability was calculated using three statistical approaches-NormFinder, BestKeeper and a comparative threshold cycle (Ct) method. The study found that of the three candidate reference genes, 28S rRNA and 18S rRNA were the most stable with no significant differences when maturity, gender and exposure to BPA were considered. It was noted that when these genes (18S rRNA, 28S rRNA, β-actin) were used for normalization using animals exposed to 10 ng BPA and the qRT-PCR method, different levels of cyp4 expression were observed. However, BPA significantly decreased the expression levels of cyp4 compared to the control (animals not exposed to BPA), only when 18S rRNA was used as an internal reference. These results highlighted the importance of selecting appropriate reference genes as the endogenous control in qRT-PCR in biomonitoring programs of BPA.

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Section: Relative Expression Levels Of Cytochrome P450 4 (Cyp4) Usingmentioning

confidence: 99%

18S rRNA, a potential reference gene in the qRT-PCR measurement of bisphenol A contamination in green mussels (Perna viridis) collected from the Gulf of Thailand

2019

ANRES

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Characterization of the molecular differential responses in marine benthic macroinvertebrates exposed to polycyclic aromatic hydrocarbons

Onyena,

Manohar,

Nkwoji

et al. 2023

Aquat Ecol

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Baseline levels of metabolites in different tissues of mussel Mytilus galloprovincialis (Bivalvia: Mytilidae)

Cappello

Giannetto

Parrino

et al. 2018

Comparative Biochemistry and Physiology Part D: Genomics and Pr

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Transcriptional variations in biomarkers ofMytilus galloprovincialissampled from Central Adriatic coastal waters (Marche region, Italy)

Abstract: the variations in gene expression observed indicated high sensitivity to pollutants, and showed differences among the sampling sites.

Cited by 5 publications

References 104 publications

18S rRNA, a potential reference gene in the qRT-PCR measurement of bisphenol A contamination in green mussels (Perna viridis) collected from the Gulf of Thailand

18S rRNA, a potential reference gene in the qRT-PCR measurement of bisphenol A contamination in green mussels (Perna viridis) collected from the Gulf of Thailand

Characterization of the molecular differential responses in marine benthic macroinvertebrates exposed to polycyclic aromatic hydrocarbons

Baseline levels of metabolites in different tissues of mussel Mytilus galloprovincialis (Bivalvia: Mytilidae)

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