Transcriptional signatures in leaves of adult European beech trees (Fagus sylvatica L.) in an experimentally enhanced free air ozone setting

Olbrich, Maren; Gerstner, Elke; Bahnweg, G.; Häberle, Karl‐Heinz; Matyssek, Rainer; Welzl, Gerhard; Heller, Werner; Ernst, D.

doi:10.1016/j.envpol.2009.08.001

Cited by 13 publications

(4 citation statements)

References 55 publications

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“…Olbrich et al ( 2010 ) studied transcriptional responses in juvenile Fagus sylvatica saplings fumigated with a double concentration of ambient O 3 over 3 years by conducting microarray hybridization. Recently, the effects of O 3 (twice ambient concentration) on F. sylvatica were investigated performing a large-scale protein analysis based on 2-D Fluorescence Difference Gel Electrophoresis (2-DE DIGE) (Kerner et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Suppression Substractive Hybridization and NGS Reveal Differential Transcriptome Expression Profiles in Wayfaring Tree (Viburnum lantana L.) Treated with Ozone

et al. 2016

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Tropospheric ozone (O3) is a global air pollutant that causes high economic damages by decreasing plant productivity. It enters the leaves through the stomata, generates reactive oxygen species, which subsequent decrease in photosynthesis, plant growth, and biomass accumulation. In order to identify genes that are important for conferring O3 tolerance or sensitivity to plants, a suppression subtractive hybridization analysis was performed on the very sensitive woody shrub, Viburnum lantana, exposed to chronic O3 treatment (60 ppb, 5 h d−1 for 45 consecutive days). Transcript profiling and relative expression assessment were carried out in asymptomatic leaves, after 15 days of O3 exposure. At the end of the experiment symptoms were observed on all treated leaves and plants, with an injured leaf area per plant accounting for 16.7% of the total surface. Cloned genes were sequenced by 454-pyrosequencing and transcript profiling and relative expression assessment were carried out on sequenced reads. A total of 38,800 and 12,495 high quality reads obtained in control and O3-treated libraries, respectively (average length of 319 ± 156.7 and 255 ± 107.4 bp). The Ensembl transcriptome yielded a total of 1241 unigenes with a total sequence length of 389,126 bp and an average length size of 389 bp (guanine-cytosine content = 49.9%). mRNA abundance was measured by reads per kilobase per million and 41 and 37 ensembl unigenes showed up- and down-regulation respectively. Unigenes functionally associated to photosynthesis and carbon utilization were repressed, demonstrating the deleterious effect of O3 exposure. Unigenes functionally associated to heat-shock proteins and glutathione were concurrently induced, suggesting the role of thylakoid-localized proteins and antioxidant-detoxification pathways as an effective strategy for responding to O3. Gene Ontology analysis documented a differential expression of co-regulated transcripts for several functional categories, including specific transcription factors (MYB and WRKY). This study demonstrates that a complex sequence of events takes place in the cells at intracellular and membrane level following O3 exposure and elucidates the effects of this oxidative stress on the transcriptional machinery of the non-model plant species V. lantana, with the final aim to provide the molecular supportive knowledge for the use of this plant as O3-bioindicator.

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Section: Introductionmentioning

confidence: 99%

Suppression Substractive Hybridization and NGS Reveal Differential Transcriptome Expression Profiles in Wayfaring Tree (Viburnum lantana L.) Treated with Ozone

et al. 2016

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“…The analysis of the variation within 'candidate' genes potentially involved in adaptation to a phenotypic trait is one possibility to investigate the genetic background of adaptation. Until now, only few studies aim to identify genes that are involved in drought stress response and bud phenology in beech (Lalagüe et al 2010), and only a limited number of beech sequences are available (Jimenez et al 2008;Olbrich et al 2005Olbrich et al , 2010Schlink 2011). Therefore, the candidate gene approach described here is based on both published F. sylvatica sequences and orthologous sequences identified in other plant species such as oaks (Gailing et al 2009;Vornam et al 2011).…”

Section: Introductionmentioning

confidence: 99%

DNA sequence variation and development of SNP markers in beech (Fagus sylvatica L.)

Seifert¹,

Vornam²,

Finkeldey³

2012

Eur J Forest Res

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European beech (Fagus sylvatica L.) is one of the most important deciduous tree species in Central Europe. The potential of beech to adapt to climate change, higher temperatures, and less precipitation in the summer months is still unknown. Most studies in beech used microsatellite, AFLP (amplified fragment length polymorphism), or isozyme markers, which have only a restricted potential to analyze adaptation. Only few studies investigated genes probably involved in the adaptation to drought stress and bud phenology in beech. In this study, SNP (single nucleotide polymorphisms) markers were developed in order to analyze adaptation and their technical advantages compared to microsatellites and AFLPs were discussed. Partial sequences of ten candidate genes probably involved in drought stress and/or bud phenology were identified at the genomic level, and SNPs and indels (insertions/deletions) in coding and non-coding regions were analyzed. Plant material was sampled along a precipitation gradient in Germany. In total, 8,145 bp were sequenced and analyzed, 4,038 bp were located in exon and 4,107 bp in intron regions. 63 SNPs and 11 indels were detected, which are differently distributed over the studied gene regions. The nucleotide diversity ranged from 0 to 6.62 (p 9 10 -3 ) and is comparable to other tree species, whereas the mean nucleotide diversity (2.64) for F. sylvatica is comparatively low. These results will help to investigate the genetic basis of drought stress and bud burst and to conduct association mapping in natural populations. Furthermore, the detected SNPs can also be used for population genetic studies.

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“…11) allows a feasible inclusion of ozone effects to photosynthesis as opposed to a direct morphology -ozone concentration correlation model. This formulation is based on the vertical distribution of canopy light (Lemoine et al, 2002) and the different response of sun leaves and shade leaves to oxidative stress from ozone (Olbrich et al, 2010). To introduce more inputs, the metabolic network model can be extended and modified, avoiding the scenario of adding parameters at the organ scale for re-calibration in a correlation-based approach.…”

Section: Scale Dependenciesmentioning

confidence: 99%

An approach to multiscale modelling with graph grammars

et al. 2014

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The graph data structure supports data representation and grammar operations at multiple scales. The results demonstrate that multiscale modelling is a viable method in FSPM and an alternative to correlation-based modelling. Advantages and disadvantages of multiscale modelling are illustrated by comparisons with single-scale implementations, leading to motivations for further research in sensitivity analysis and run-time efficiency for these models.

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Transcriptional signatures in leaves of adult European beech trees (Fagus sylvatica L.) in an experimentally enhanced free air ozone setting

Cited by 13 publications

References 55 publications

Suppression Substractive Hybridization and NGS Reveal Differential Transcriptome Expression Profiles in Wayfaring Tree (Viburnum lantana L.) Treated with Ozone

Suppression Substractive Hybridization and NGS Reveal Differential Transcriptome Expression Profiles in Wayfaring Tree (Viburnum lantana L.) Treated with Ozone

DNA sequence variation and development of SNP markers in beech (Fagus sylvatica L.)

An approach to multiscale modelling with graph grammars

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