Transcriptional responses to 2,4-D herbicide treatment of a Eurasian (Myriophyllum spicatum) and a hybrid (M. spicatum × M. sibiricum) genotype of watermilfoil that differ in their sensitivity to 2,4-D

Chorak, Gregory M.; Correr, Fernando Henrique; Lachowiec, Jennifer; Reynolds, Gillian; Thum, Ryan A.

doi:10.1016/j.aquabot.2023.103631

Cited by 3 publications

(3 citation statements)

References 30 publications

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“…Overall, measured by the number of DEGs at each time point in each year, the response of 'UK2014' to 2,4-D was less than that of 'Kenland'. This result was similar to that observed for the phenotypic response between 'Kenland and 'UK2014' by Araujo et al [41] and between the more and less 2,4-D sensitive genotypes of Eurasian watermilfoil (Myriophyllum spicatum) [45].…”

Section: Effect Of 24-d On Gene Expressionsupporting

confidence: 90%

“…Our gene ontology analysis agrees with these reports, but a decrease in photosynthesis transcripts occurred later than 6 HAT. Further, in the more 2,4-D sensitive Eurasian genotype of watermilfoil, compared to the less 2,4-D sensitive watermilfoil hybrid genotype (M. spicatum × M. sibiricum), the downregulation of photosynthetic genes following 2,4-D treatment was more pronounced in the sensitive genotype [45]. McCauley et al [22] also suggested a whole-scale downregulation of the photosynthetic process in plants treated with auxin herbicides.…”

Section: Deg Ontologymentioning

confidence: 99%

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Comparative Gene Expression following 2,4-D Treatment in Two Red Clover (Trifolium pratense L.) Populations with Differential Tolerance to the Herbicide

Araujo,

Barrett,

Dinkins

2024

Agronomy

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Incorporation of red clover (Trifolium pratense L.) into grass pastures can reduce the need for nitrogen fertilizer applications and increase the nutritional value of the forage. However, red clover cultivars available for Kentucky producers are highly susceptible to herbicides, such as 2,4-D (2,4-dichlorophenoxy acetic acid), used for pasture broadleaf weed control. To overcome this problem, ‘UK2014’ red clover was selected for increased tolerance to 2,4-D. We employed a transcriptome analysis approach to compare the gene expression response following 2,4-D treatment of ‘UK2014’ to that of ‘Kenland’, a 2,4-D sensitive red clover and one of the parents of ‘UK2014’. The objectives were to first determine if the increased 2,4-D tolerance in ‘UK2014’ is reflected in a change of transcription response and/or a quicker recovery of a transcriptional response following 2,4-D treatment, and second, to identify genes, whether constitutively expressed or induced by 2,4-D, which could be the basis for the increased 2,4-D tolerance. Leaf tissue from the two red clovers grown in the field was collected at 4, 24, and 72 h after 2,4-D (1.12 kg 2,4-amine a.e. ha−1) treatment from both untreated and treated plants. Global gene expression was determined with reads from Illumina Hiseq 2500 mapped against the red clover draft genome, Tpv2.1 (GenBank Accession GCA_900079335.1). Genes that displayed differential expression (DEGs) following 2,4-D treatment were selected for further analysis. The number of DEGs was higher for ‘Kenland’ than for ‘UK2014’, suggesting that a lower transcriptional response corresponds with the higher 2,4-D tolerance in the ‘UK2014’ line. Similarly, gene ontology enrichment analysis revealed that expression of photosynthesis-related genes was less affected by 2,4-D in the ‘UK2014’ line than ‘Kenland’. Although we were not able to identify any specific genes that are the basis for the increased 2,4-D tolerance of ‘UK2014’, we concluded that the increased 2,4-D tolerance of ‘UK2014’ correlates with a decreased transcription response to 2,4-D. Additionally, expression of several cytochrome P450 genes that had different isoforms between ‘UK2014’ and ‘Kenland’ increased significantly in both following 2,4-D treatment, one or more of these P450s could be mediators of 2,4-D metabolism and tolerance in red clover.

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Section: Effect Of 24-d On Gene Expressionsupporting

confidence: 90%

Section: Deg Ontologymentioning

confidence: 99%

Comparative Gene Expression following 2,4-D Treatment in Two Red Clover (Trifolium pratense L.) Populations with Differential Tolerance to the Herbicide

Araujo,

Barrett,

Dinkins

2024

Agronomy

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“…It is noteworthy that M. aquaticum exhibits significantly higher dry weight, and relatively higher growth rates than other species when exposed to various butachlor concentrations, suggesting that M. aquaticum is more capable of withstanding butachlor's presence. This outcome aligns with past research, which has demonstrated that invasive species tend to be more vulnerable to the herbicide flurpyrauxifen-benzyl compared to their native counterparts [45,46]. Under natural conditions, the Fv/Fm ratios of the four studied species were between 0.6 to 0.8.…”

Section: The Growth and Physiology Of Four Aquatic Plants In Response...supporting

confidence: 90%

Negative Effects of Butachlor on the Growth and Physiology of Four Aquatic Plants

Huang,

Zhao,

Xian

et al. 2024

Plants

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The increasing use of herbicides in intelligent agricultural production is driven by the time-consuming nature of manual weeding, as well as its ephemeral effectiveness. However, herbicides like butachlor degrade slowly and can be washed away by rainwater, ultimately flowing into the farm ponds and posing risks to aquatic plants. To identify and recommend superior restoration strategies that effectively address the challenges posed by butachlor, we investigated the impacts of butachlor on the growth and physiology of four common aquatic plants (i.e., Hydrilla verticillata, Ceratophyllum demersum, Potamogeton maackianus, and Myriophyllum aquaticum) and their potential role in mitigating environmental damage by reducing residual herbicide levels. Our findings indicated that M. aquaticum was tolerant to butachlor, exhibiting higher growth rates than other species when exposed to various butachlor concentrations. However, the concentration of butachlor negatively impacted the growth of H. verticillata, C. demersum, and P. maackianus, with higher concentrations leading to more significant inhibitory effects. After a 15-day experimental period, aquatic plants reduced the butachlor residuals in culture mediums across concentrations of 0.5 mg/L, 1 mg/L, and 2 mg/L compared to non-plant controls. Our findings classified P. maackianus as butachlor-sensitive and M. aquaticum as butachlor-tolerant species. This investigation represents novel research aimed at elucidating the contrasting effects of different concentrations of butachlor on four common aquatic species in the agricultural multi-pond system.

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Short-term test for the toxicogenomic assessment of ecotoxic modes of action in Myriophyllum spicatum

Hanfland,

Lousberg,

Ringbeck

et al. 2024

Science of The Total Environment

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Transcriptional responses to 2,4-D herbicide treatment of a Eurasian (Myriophyllum spicatum) and a hybrid (M. spicatum × M. sibiricum) genotype of watermilfoil that differ in their sensitivity to 2,4-D

Cited by 3 publications

References 30 publications

Comparative Gene Expression following 2,4-D Treatment in Two Red Clover (Trifolium pratense L.) Populations with Differential Tolerance to the Herbicide

Comparative Gene Expression following 2,4-D Treatment in Two Red Clover (Trifolium pratense L.) Populations with Differential Tolerance to the Herbicide

Negative Effects of Butachlor on the Growth and Physiology of Four Aquatic Plants

Short-term test for the toxicogenomic assessment of ecotoxic modes of action in Myriophyllum spicatum

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