Transcriptional responses of soybean aphids to sublethal insecticide exposure

Enders, Laramy; Rault, Leslie C.; Heng-Moss, Tiffany; Siegfried, Blair D.; Miller, Nicholas J.

doi:10.1016/j.ibmb.2019.103285

Cited by 12 publications

(9 citation statements)

References 29 publications

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“…Recently, the analysis of transcriptomic databases allowed the identification of new relevant components of the detoxifying response to insecticides in mosquitoes [21][22][23]. Studies addressing the transcriptomic response after a treatment with an insecticide in hemipterans are scarce (see for example [24,25]) and none of them have been performed in a vector of Chagas disease to date.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic modulation in response to an intoxication with deltamethrin in a population of Triatoma infestans with low resistance to pyrethroids

et al. 2022

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Background Triatoma infestans is the main vector of Chagas disease in the Southern Cone. The resistance to pyrethroid insecticides developed by populations of this species impairs the effectiveness of vector control campaigns in wide regions of Argentina. The study of the global transcriptomic response to pyrethroid insecticides is important to deepen the knowledge about detoxification in triatomines. Methodology and findings We used RNA-Seq to explore the early transcriptomic response after intoxication with deltamethrin in a population of T. infestans which presents low resistance to pyrethroids. We were able to assemble a complete transcriptome of this vector and found evidence of differentially expressed genes belonging to diverse families such as chemosensory and odorant-binding proteins, ABC transporters and heat-shock proteins. Moreover, genes related to transcription and translation, energetic metabolism and cuticle rearrangements were also modulated. Finally, we characterized the repertoire of previously uncharacterized detoxification-related gene families in T. infestans and Rhodnius prolixus. Conclusions and significance Our work contributes to the understanding of the detoxification response in vectors of Chagas disease. Given the absence of an annotated genome from T. infestans, the analysis presented here constitutes a resource for molecular and physiological studies in this species. The results increase the knowledge on detoxification processes in vectors of Chagas disease, and provide relevant information to explore undescribed potential insecticide resistance mechanisms in populations of these insects.

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Section: Introductionmentioning

confidence: 99%

Transcriptomic modulation in response to an intoxication with deltamethrin in a population of Triatoma infestans with low resistance to pyrethroids

et al. 2022

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“…Secondary metabolites, such as avonoids, terpenoids, phenols and alkaloids, have the characteristics of antibiotics and thus play an important role in the defence of phytophagous insects [34,35]. Terpenoids, avonoids and tannins enhance plant defence against aphids by affecting aphid colonization [36][37][38][39]. Our metabolomics results highlighted differential changes in 15 major classes of metabolites.…”

Section: Metabolomics Study For Disease Resistancementioning

confidence: 99%

Transcriptomic and Metabolomic Changes Triggered by Macrosiphum Rosivorum in Rose (Rosa longicuspis)

Gao

Zhang

Yan

et al. 2021

Preprint

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Background: Rose is an important economic horticultural crop. However, its field growth and quality are negatively affected by aphids. However, the defence mechanisms used by rose plants against aphids are unclear. A previous study showed that Macrosiphum rosivorum is the most common and harmful aphid species to Rosa plants and that Rosa longicuspis is highly resistant species to aphids. Therefore, to understand the defence mechanism of rose under aphid stress, we combined RNA sequencing and metabolomics techniques to investigate the changes in gene expression and metabolomic processes in R. longicuspis infected with M. rosivorum. Result: In our study, after inoculation with M. rosivorum, M. rosivorum quickly colonized. A total of 34202 genes and 758 metabolites were detected in all samples, with 2845, 2627 and 466 differentially expressed genes (DEGs) were found in CK-vs.-A3 d, CK-vs.-A5 d, and A3 d-vs.-A5 d, respectively. Among these metabolites, 65, 70 and 26 differentially expressed metabolites (DEMs) were found in CK-vs.-A3 d, CK-vs.-A5 d, and A3 d-vs.-A5 d, respectively. The combined omics approach revealed that M. rosivorum is perceived by effector-triggered immunity. Under M. rosivorum stress, R. longicuspis responded by signal transduction pathway activation, transcription factor expression, ROS production and hormone-mediated defence responses. Interestingly, the ‘brassinosteroid biosynthesis’ pathway was significantly enriched in A3 d-vs.-A5 d. Further analysis showed that M. rosivorum induced the transformation of starch and sucrose, the biosynthesis of terpenoids, tannins and phenolic acids and metabolism of cyanoamino acid. Importantly, the ‘tropane, piperidine and pyridine alkaloid biosynthesis’, ‘glutathione metabolic’ and ‘glucosinolate biosynthesis’ pathways were significantly enriched, which resulted in increased levels of metabolites that were involved in the plant defence response. Conclusion: Our study provides candidate genes and metabolites for Rosa defence against aphids. What’s more, this study provides a theoretical basis for further exploring the molecular regulation mechanism of rose aphid resistance and aphid resistance breeding in the future.

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“…Recently, the analysis of transcriptomic databases allowed the identification of new relevant components of the detoxifying response to insecticides in mosquitoes [20][21][22]. Studies addressing the transcriptomic response after a treatment with an insecticide in hemipterans are scarce (see for example [23,24]) and none of them have been performed in a vector of Chagas disease to date.…”

Section: Vectorial Transmission Of T Cruzi Occurs In Wide Zones Of the Southern Cone Wherementioning

confidence: 99%

Transcriptomic modulation in response to an intoxication with deltamethrin in Triatoma infestans, vector of Chagas disease

Traverso

Estivalis

Fernandes

et al. 2021

Preprint

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Background: Triatoma infestans is the main vector of Chagas disease in the Southern Cone. The resistance to pyrethroid insecticides developed by populations of this species impairs the effectiveness of vector control campaigns in wide regions of Argentina. The study of the global transcriptomic response to pyrethroid insecticides is important to deepen the knowledge about detoxification in triatomines. Methodology and findings: We used RNA-Seq to explore the early transcriptomic response of T. infestans after intoxication with deltamethrin. We were able to assemble a complete transcriptome of this vector and found evidence of differentially expressed genes belonging to diverse families such as chemosensory and odorant-binding proteins, ABC transporters and heat-shock proteins. Moreover, genes related to transcription and translation, energetic metabolism and cuticle rearrangements were also modulated. Finally, we characterized the repertoire of previously uncharacterized detoxification-related gene families in T. infestans and Rhodnius prolixus. Conclusions and significance: Our work contributes to the understanding of the detoxification response in vectors of Chagas disease. Given the absence of genomic information from T. infestans, the analysis presented here constitutes a resource for molecular and physiological studies in this species. The results increase the knowledge on detoxification processes in vectors of Chagas disease, and provide relevant information to explore new potential insecticide resistance mechanisms in these insects.

show abstract

Transcriptional responses of soybean aphids to sublethal insecticide exposure

Cited by 12 publications

References 29 publications

Transcriptomic modulation in response to an intoxication with deltamethrin in a population of Triatoma infestans with low resistance to pyrethroids

Transcriptomic modulation in response to an intoxication with deltamethrin in a population of Triatoma infestans with low resistance to pyrethroids

Transcriptomic and Metabolomic Changes Triggered by Macrosiphum Rosivorum in Rose (Rosa longicuspis)

Transcriptomic modulation in response to an intoxication with deltamethrin in Triatoma infestans, vector of Chagas disease

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