Transcriptional Responses of Sodium-Silicate-Induced Potato Resistance Against Rhizoctonia solani AG-3

Abstract

Stem canker and black scurf of potatoes, caused by Rhizoctonia solani, is a highly destructive worldwide. In controlling the disease, the application of sodium silicate in potato fields has shown promise in improving potato resistance against R. solani, although the underlying mechanism remains unclear. In this study, we used RNA sequencing analysis to examine the transcriptome of potato subterraneous stems of potato plants. These stems were both inoculated with R. solani and treated with sodium silicate, while a control group received no sodium silicate treatment. Transcriptome analysis was performed at 4, 8, and 12 days post-application (Group SS) and compared with the control (Group CK). A total of 1491 genes were identified as differentially expressed genes (DEGs). Furthermore, these DEGs are involved in hydrolase activity, plant-pathogen interactions, hormone signal transduction, and the phenylpropanoid biosynthesis pathway. These findings suggest that the application of sodium silicate induces a complex defense network in plants, involving physical barriers, innate immunity, phytohormone signaling, and various phenylpropanoid compounds, to combat R. solani infection. This study provides valuable insights into the molecular mechanisms underlying sodium silicate-induced resistance and its potential for reducing stem canker and black scurf in potato crops.