Transcriptional Responses of Chilean Quinoa (Chenopodium quinoa Willd.) Under Water Deficit Conditions Uncovers ABA-Independent Expression Patterns

Morales, Andrea; Zurita‐Silva, Andrés; Maldonado, Jonathan; Silva, Herman

doi:10.3389/fpls.2017.00216

Cited by 60 publications

(56 citation statements)

References 91 publications

(114 reference statements)

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“…Using next-generation RNA sequencing (NGS) technology, numerous drought-responsive genes have been identified in many non-model plant species including Pinus halepensis [15], Chenopodium quinoa (Willd.) [16], and Ammopiptanthus mongolicus [17], but these studies mainly focused on single tissues or whole plants, except for Prunus mahaleb, Arundo donax, Lens culinaris, and Prunus persica, for which NGS was used to recognize the response mechanisms coordinated between leaves and roots under drought stress [18][19][20][21][22]. These studies indicated that hormone signal transduction plays a considerable role in the plant response to drought stress.…”

Section: Introductionmentioning

confidence: 99%

Coordinated mechanisms of leaves and roots in response to drought stress underlying full-length transcriptome profiling in Vicia sativa L

et al. 2020

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Background: Common vetch (Vicia sativa L.) is an important self-pollinating annual forage legume and is of interest for drought prone regions as a protein source to feed livestock and human consumption. However, the development and production of common vetch are negatively affected by drought stress. Plants have evolved common or distinct metabolic pathways between the aboveground and underground in response to drought stress. Little is known regarding the coordinated response of aboveground and underground tissues of common vetch to drought stress. Results: Our results showed that a total of 30,427 full-length transcripts were identified in 12 samples, with an average length of 2278.89 bp. Global transcriptional profiles of the above 12 samples were then analysed via Illumina-Seq. A total of 3464 and 3062 differentially expressed genes (DEGs) were identified in the leaves and roots, respectively. Gene Ontology (GO) enrichment analyses identified that the dehydrin genes and Δ 1 -pyrroline-5-carboxylate synthase were induced for the biosynthesis of proline and water conservation. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis results indicated that the DEGs were significantly enriched in hormone signal transduction, starch and sucrose metabolism, and arginine and proline metabolism, and various drought response candidate genes were also identified. Abscisic acid (ABA; the AREB/ABF-SnRK2 pathway) regulates the activity of AMY3 and BAM1 to induce starch degradation in leaves and increase carbon export to roots, which may be associated with the drought stress responses in common vetch. Among the co-induced transcription factors (TFs), AREB/ABF, bHLH, MYB, WRKY, and AP2/ERF had divergent expression patterns and may be key in the crosstalk between leaves and roots during adaption to drought stress. In transgenic yeast, the overexpression of four TFs increased yeast tolerance to osmotic stresses. Conclusion:The multipronged approach identified in the leaves and roots broadens our understanding of the coordinated mechanisms of drought response in common vetch, and further provides targets to improve drought resistance through genetic engineering.

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Section: Introductionmentioning

confidence: 99%

Coordinated mechanisms of leaves and roots in response to drought stress underlying full-length transcriptome profiling in Vicia sativa L

et al. 2020

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“…N supply affected all fluorescence parameters measured and different interactions among factors were observed according to the parameter studied ( Table 1 ). The Fv/Fm ratio, which reflects the maximum quantum efficiency of photosystem II, has been demonstrated to be stable in quinoa, despite water stress (Morales et al, 2017). Amongst the treatments and time points in the three quinoa cultivars used in this study, the Fv/Fm ratio was maintained higher than 0.7, which is considered an optimal value ( Figures 4A–C ).…”

Section: Discussionmentioning

confidence: 99%

“…In fact, coastal/lowland Chilean genotypes have been used as parental elite sources in European quinoa breeding programs (Jacobsen and Stølen, 1993; Jacobsen, 1997). Chilean quinoa lowland genotypes present high phenotypic variability, agronomical performance, and tolerance to stress conditions (Berti et al, 2000; Ruiz-Carrasco et al, 2011; Bascuñán-Godoy et al, 2016; Morales et al, 2017; Gonzalez-Teuber et al, 2018). …”

Section: Introductionmentioning

confidence: 99%

Nitrogen Supply Affects Photosynthesis and Photoprotective Attributes During Drought-Induced Senescence in Quinoa

et al. 2018

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Drought during senescence has become more common in Mediterranean climates in recent years. Chenopodium quinoa Willd has been identified as tolerant to poor soil conditions and drought. Previous observations have found that sufficient nitrogen (N) supply mitigates yield losses under terminal drought conditions. However, there is no understanding of the mechanisms behind this effect. We hypothesized that N up-regulates both photosynthetic and photoprotective elements during drought-induced senescence, alleviating the negative impact of drought on yield. The role of N supply and terminal drought on photoprotection was tested using three Chilean quinoa genotypes from different climatic zones: Faro, UdeC9, and BO78. Plants were grown under high nitrogen (HN) or low nitrogen (LN) conditions and subjected to terminal drought at the onset of senescence. Photosynthetic and photochemical and non-photochemical processes were evaluated at both the onset of drought and after 15 days of drought conditions. N supplementation modified most of the physiological parameters related to photochemical dissipation of energy, photosynthesis, and yield in quinoa. In contrast, water restriction did not affect photosynthesis in quinoa, and its effect on yield was dependent on the genotype. A significant interaction N × G was observed in photosynthesis, relative water content, protein content, Fv/Fm, and chlorophylls. In general, Faro was able to maintain higher levels of these attributes under LN conditions than UdeC9 and BO78. In addition, the interacting effects of N × W regulated the level of most pigments in quinoa as well as the photoprotective induction of non-photochemical quenching (NPQ) during senescence. During terminal drought at LN conditions, Faro presented a larger NPQ induction under drought conditions than UdeC9 and BO78, which was supported by a larger zeaxanthin content and de-epoxidation state of the xanthophyll pool. Interestingly, BO78 did not induce NPQ in response to drought-induced senescence but instead enhanced the content of betacyanins. This response needs to be researched in future works. Finally, we observed that LN supply reduced the correlationship between the de-epoxidation state of the xanthophyll cycle and NPQ. This could be an indication that N supply not only compromised the capacity for photosynthetic performance in quinoa plants, but also affected the plasticity of thermal dissipation, restricting further changes during drought-induced senescence.

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“…Sustaining quinoa yields in climates with frequent heat waves will depend, in large part, on breeding heat‐tolerant varieties. These efforts are hindered by the lack of suitable markers that can be used in breeding programs for heat and drought tolerance (Raney et al ., ; Morales et al ., ). Here, we aim to identify such markers.…”

Section: Introductionmentioning

confidence: 97%

Impact of heat and drought stress on peroxisome proliferation in quinoa

et al. 2019

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AS and KM are joint senior authors. SUMMARYAlthough peroxisomes play a key role in plant metabolism under both normal and stressful growth conditions, the impact of drought and heat stress on the peroxisomes remains unknown. Quinoa represents an informative system for dissecting the impact of abiotic stress on peroxisome proliferation because it is adapted to marginal environments. Here we determined the correlation of peroxisome abundance with physiological responses and yield under heat, drought and heat plus drought stresses in eight genotypes of quinoa. We found that all stresses caused a reduction in stomatal conductance and yield. Furthermore, H 2 O 2 content increased under drought and heat plus drought. Principal component analysis demonstrated that peroxisome abundance correlated positively with H 2 O 2 content in leaves and correlated negatively with yield. Pearson correlation coefficient for yield and peroxisome abundance (r = À0.59) was higher than for commonly used photosynthetic efficiency (r = 0.23), but comparable to those for classical stress indicators such as soil moisture content (r = 0.51) or stomatal conductance (r = 0.62). Our work established peroxisome abundance as a cellular sensor for measuring responses to heat and drought stress in the genetically diverse populations. As heat waves threaten agricultural productivity in arid climates, our findings will facilitate identification of genetic markers for improving yield of crops under extreme weather patterns. Gene transcription analysisPeroxisome genes in quinoa were identified by performing a BLAST search of Arabidopsis sequences against the quinoa pseudomolecule genome assembly (Jarvis et al., 2017) using default parameters ( Figure S3). Syntenic regions of coding sequences

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Transcriptional Responses of Chilean Quinoa (Chenopodium quinoa Willd.) Under Water Deficit Conditions Uncovers ABA-Independent Expression Patterns

Cited by 60 publications

References 91 publications

Coordinated mechanisms of leaves and roots in response to drought stress underlying full-length transcriptome profiling in Vicia sativa L

Coordinated mechanisms of leaves and roots in response to drought stress underlying full-length transcriptome profiling in Vicia sativa L

Nitrogen Supply Affects Photosynthesis and Photoprotective Attributes During Drought-Induced Senescence in Quinoa

Impact of heat and drought stress on peroxisome proliferation in quinoa

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