Transcriptional responses in spleens from mice exposed to Yersinia pestis CO92

Rogers, James V.; Choi, Young Wook; Giannunzio, Lenore F.; Sabourin, Patrick J.; Bornman, Daniel M.; Blosser, Emily G.; Sabourin, Carol L.

doi:10.1016/j.micpath.2007.04.001

Cited by 16 publications

(14 citation statements)

References 89 publications

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“…B. suis, B. pseudomallei, F. tularensis, and Y. pestis can persist outside a host for extended periods of time (Bossi et al, 2004;Inglis & Sagripanti, 2006;Sinclair et al, 2008), and their respective estimated infectious doses can range from less than 10 to thousands of organisms (Bossi et al, 2004;Hoppe et al, 1999;Lathem et al, 2005;Rogers et al, 2007;Russell et al, 2000;Saslaw & Carlisle, 1969;Sawyer et al, 1966;Welkos et al, 1995;Welkos et al, 1997). Based on the lowest log10 CFU detected for each of these organisms in the decontamination controls in this study, the concentration of viable bacteria recovered from each of the test materials falls within the respective estimated infectious dose range for each of these bacterial species.…”

Section: Discussionmentioning

confidence: 99%

Inactivation of Brucella Suis, Burkholderia pseudomallei, Francisella tularensis, and Yersinia pestis using Vaporous Hydrogen Peroxide

et al. 2010

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This study evaluated the inactivation of Brucella suis, Burkholderia pseudomallei, Francisella tularensis, and Yersinia pestis on glass, Hypalon ® rubber glove, and stainless steel using vaporous hydrogen peroxide fumigation of a ~15 m 3 chamber. A suspension of approximately 1 x 10 8 colony forming units (CFU) of each organism was dried on coupons of each type of test surface and exposed to vaporous hydrogen peroxide. A significant reduction in the log10 CFU of each organism on all test materials was observed between the controls evaluated after a 1-hour drying time and unexposed controls evaluated after decontamination. For all organisms, qualitative growth assessments showed that vaporous hydrogen peroxide exposure completely inactivated bacterial viability on all replicates of the test materials incubated up to 7 days post-exposure. In parallel, all Geobacillus stearothermophilus biological indicators (BI) exposed to vaporous hydrogen peroxide exhibited no growth after 1 and 7 days incubation. This study provides information on using a combination of quantitative and qualitative growth assessments to evaluate vaporous hydrogen peroxide for the surface decontamination of B. suis, B. pseudomallei, F. tularensis, and Y. pestis within a large-scale chamber. ArticlesInactivation of Brucella suis, Burkholderia pseudomallei, Francisella tularensis, and Yersinia pestis Using Vaporous Hydrogen Peroxide

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Section: Discussionmentioning

confidence: 99%

Inactivation of Brucella Suis, Burkholderia pseudomallei, Francisella tularensis, and Yersinia pestis using Vaporous Hydrogen Peroxide

et al. 2010

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“…One of the genes identified was IQGAP1, which was downregulated 5.6‐fold [16]. Proteomic analysis later showed reduced IQGAP1 expression in murine splenic tissue after infection by Yersinia pestis [17], suggesting that IQGAP1 may be a target for pathogen‐induced changes in the host cell. Consistent with this postulate, IQGAP1 is known to interact with numerous proteins that functionally link pathogenic microbes to host cell invasion (Table 1 ).…”

Section: Iqgap1 and Microbial Pathogenesismentioning

confidence: 99%

IQGAP1 in microbial pathogenesis: Targeting the actin cytoskeleton

Kim¹,

White²,

Sacks³

2011

FEBS Letters

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Microbial pathogens continue to cause widespread morbidity and mortality. Central to the pathogens' virulence is manipulation of the host cell's cytoskeleton, which facilitates microbial invasion, multiplication, and avoidance of the innate immune response. IQGAP1 is a ubiquitously expressed scaffold protein that integrates diverse signaling cascades. Research has shown that IQGAP1 binds to and modulates the activity of multiple proteins that participate in bacterial invasion. Here, we review data that support a role for IQGAP1 in infectious disease via its ability to regulate the actin cytoskeleton. In addition, we explore other mechanisms by which IQGAP1 may be exploited by microbial pathogens.

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“…pestis inactivation on materials Y. pestis as a result of the initial drying of the inoculum as well as the time period in which the nondecontaminated controls were maintained in parallel to the decontamination run. The implementation of both quantitative and qualitative assessments was performed based on previous studies where Y. pestis can persist from hours to days on contaminated surfaces and in the environment (Wilkinson 1966;Rose et al 2003;Sinclair et al 2008) and the LD 50 values for Y. pestis CO92 have been reported to range from 1 to 2 · 10 4 CFU that varies based on the route of exposure (Welkos et al 1995(Welkos et al , 1997Benner et al 1999;Lathem et al 2005;DeBord et al 2006;Rogers et al 2007a). The present study demonstrated significant decreases in recoverable, viable Y. pestis in the decontamination controls, supporting previous work in which the viability of Y. pestis decreases with time following drying on various material matrices (Wilkinson 1966;Rose et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Vapour-phase hydrogen peroxide inactivatesYersinia pestisdried on polymers, steel, and glass surfaces

Rogers

Richter

Shaw

et al. 2008

Letters in Applied Microbiology

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Aims: This study evaluated the inactivation of virulent Yersinia pestis dried on polymers, steel, and glass surfaces using vapour‐phase hydrogen peroxide. Methods and Results: A suspension of Y. pestis CO92 (1·70 × 108 CFU) was dried on 10 different types of test surfaces and exposed to vapour‐phase hydrogen peroxide fumigation for a contact time of 2 h. A significant reduction in the log10 CFU of Y. pestis on all 10 materials was observed between the controls evaluated after a 1 h drying time and unexposed controls evaluated after the decontamination run. Qualitative growth assessment showed that vapour‐phase hydrogen peroxide exposure inactivated Y. pestis on all replicates of the 10 test materials as well as biological indicators up to 7 days postexposure. Conclusions: Virulent Y. pestis CO92 is inactivated on polymers, steel, and glass surfaces when exposed to vapour‐phase hydrogen peroxide without observable physical damage to the test materials. Significance and Impact of the Study: This study provides information for using vapour‐phase hydrogen peroxide as a practical process for the decontamination of virulent Y. pestis in circumstances where time‐dependent attenuation/inactivation or liquid/heat decontamination may not be the most suitable approach.

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Transcriptional responses in spleens from mice exposed to Yersinia pestis CO92

Cited by 16 publications

References 89 publications

Inactivation of Brucella Suis, Burkholderia pseudomallei, Francisella tularensis, and Yersinia pestis using Vaporous Hydrogen Peroxide

Inactivation of Brucella Suis, Burkholderia pseudomallei, Francisella tularensis, and Yersinia pestis using Vaporous Hydrogen Peroxide

IQGAP1 in microbial pathogenesis: Targeting the actin cytoskeleton

Vapour-phase hydrogen peroxide inactivatesYersinia pestisdried on polymers, steel, and glass surfaces

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