Transcriptional response to nitrosative stress in <i>Saccharomyces cerevisiae</i>

Horan, Susannah; Bourges, Ingrid; Meunier, Brigitte

doi:10.1002/yea.1372

Cited by 39 publications

(27 citation statements)

References 32 publications

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“…4D). Supporting the suggested correlation between NO production and the increase of ROS, cell treatment with the NO donor DETA/NO, previously used to induce nitrosative stress in yeast cells (Horan et al, 2006), resulted in intracellular ROS accumulation (data not shown).…”

Section: Resultssupporting

confidence: 70%

“…Briefly, untreated, H 2 O 2 -treated with or without pre-incubation with L-NAME, and aged cells were disrupted using glass beads as previously described (Gourlay et al, 2003). As a positive control, cellular nitrosative stress was induced by the NO donor (Cahuana et al, 2004;Horan et al, 2006) diethylenetriamine/NO (DETA/NO, Sigma-Aldrich). The treatment with a NO donor facilitated the increase of intracellular NO levels allowing the determination of GAPDH S-nitrosation independent of H 2 O 2 .…”

Section: Detection Of S-nitrosated Gapdhmentioning

confidence: 99%

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NO-mediated apoptosis in yeast

Almeida

Büttner

Ohlmeier

et al. 2007

Journal of Cell Science

117

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Nitric oxide (NO) is a small molecule with distinct roles in diverse physiological functions in biological systems, among them the control of the apoptotic signalling cascade. By combining proteomic, genetic and biochemical approaches we demonstrate that NO and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) are crucial mediators of yeast apoptosis. Using indirect methodologies and a NO-selective electrode, we present results showing that H2O2-induced apoptotic cells synthesize NO that is associated to a nitric oxide synthase (NOS)-like activity as demonstrated by the use of a classical NOS kit assay. Additionally, our results show that yeast GAPDH is a target of extensive proteolysis upon H2O2-induced apoptosis and undergoes S-nitrosation. Blockage of NO synthesis with Nω-nitro-L-arginine methyl ester leads to a decrease of GAPDH S-nitrosation and of intracellular reactive oxygen species (ROS) accumulation, increasing survival. These results indicate that NO signalling and GAPDH S-nitrosation are linked with H2O2-induced apoptotic cell death. Evidence is presented showing that NO and GAPDH S-nitrosation also mediate cell death during chronological life span pointing to a physiological role of NO in yeast apoptosis.

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Section: Resultssupporting

confidence: 70%

Section: Detection Of S-nitrosated Gapdhmentioning

confidence: 99%

NO-mediated apoptosis in yeast

Almeida

Büttner

Ohlmeier

et al. 2007

Journal of Cell Science

117

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“…The antimicrobial effect of acidified nitrite has, as yet, been attributed mainly to the nitrogen oxides causing alteration and/or inactivation of DNA, proteins, and membrane lipids (5,16,19,20).…”

Section: Nitrite (Nomentioning

confidence: 99%

Intracellular pH Homeostasis Plays a Role in the Tolerance of Debaryomyces hansenii and Candida zeylanoides to Acidified Nitrite

Mortensen

Jacobsen

Koch

et al. 2008

Appl Environ Microbiol

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The effects of acidified-nitrite stress on the growth initiation and intracellular pH (pH i ) of individual cells of Debaryomyces hansenii and Candida zeylanoides were investigated. Our results show that 200 g/ml of nitrite caused pronounced growth inhibition and intracellular acidification of D. hansenii at an external pH (pH ex ) value of 4.5 but did not at pH ex 5.5. These results indicate that nitrous acid as such plays an important role in the antifungal effect of acidified nitrite. Furthermore, both yeast species experienced severe growth inhibition and a pH i decrease at pH ex 4.5, suggesting that at least some of the antifungal effects of acidified nitrite may be due to intracellular acidification. For C. zeylanoides, this phenomenon could be explained in part by the uncoupling effect of energy generation from growth. Debaryomyces hansenii was more tolerant to acidified nitrite at pH ex 5.5 than C. zeylanoides, as determined by the rate of growth initiation. In combination with the fact that D. hansenii was able to maintain pH i homeostasis at pH ex 5.5 but C. zeylanoides was not, our results suggest that the ability to maintain pH i homeostasis plays a role in the acidified-nitrite tolerance of D. hansenii and C. zeylanoides. Possible mechanisms underlying the different abilities of the two yeast species to maintain their pH i homeostasis during acidified-nitrite stress, comprising the intracellular buffer capacity and the plasma membrane ATPase activity, were investigated, but none of these mechanisms could explain the difference.

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“…Additionally, to deal with the stresses that accompany incompatibility, A. areolatum appears to activate the expression of various systems to protect itself. Several of the identified ESTs encoded putative proteins that are directly involved in the sensing of stress and in the defence response (Iakovlev and Stenlid, 2000;Kültz, 2005;Horan et al, 2006;Gucciardo et al, 2007). Proteins known to be involved in the nutrient starvation response (Schmelzle et al, 2004;Aro et al, 2005, Hewald et al, 2006 were also identified, which is comparable to that found in P. anserina (Federovo et al, 2005;Pinan-Lucarré et al, 2003, 2007.…”

Section: Discussionmentioning

confidence: 83%

Gene expression associated with vegetative incompatibility in Amylostereum areolatum

Nest¹,

Steenkamp²,

Slippers³

et al. 2011

Fungal Genetics and Biology

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In filamentous fungi, vegetative compatibility among individuals of the same species is determined by the genes encoded at the heterokaryon incompatibility (het) loci. The hyphae of genetically similar individuals that share the same allelic specificities at their het loci are able to fuse and intermingle, while different allelic specificities at the het loci result in cell death of the interacting hyphae. In this study, suppression subtractive hybridization (SSH) followed by pyrosequencing and quantitative reverse transcription PCR were used to identify genes that are selectively expressed when vegetatively incompatible individuals of Amylostereum areolatum interact. The SSH library contained genes associated with various cellular processes, including cell-cell adhesion, stress and defence responses, as well as cell death. Some of the transcripts encoded proteins that were previously implicated in the stress and defence responses associated with vegetative incompatibility. Other transcripts encoded proteins known to be associated with programmed cell death, but have not previously been linked with vegetative incompatibility.Results of this study have considerably increased our knowledge of the processes underlying vegetative incompatibility in Basidiomycetes in general and A. areolatum in particular.

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Transcriptional response to nitrosative stress in Saccharomyces cerevisiae

Cited by 39 publications

References 32 publications

NO-mediated apoptosis in yeast

NO-mediated apoptosis in yeast

Intracellular pH Homeostasis Plays a Role in the Tolerance of Debaryomyces hansenii and Candida zeylanoides to Acidified Nitrite

Gene expression associated with vegetative incompatibility in Amylostereum areolatum

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