Transcriptional response reveals translation machinery as target for high pressure in Lactobacillus sanfranciscensis

Pavlovic, Melanie; Hörmann, Sebastian; Vogel, Rudi F.; Ehrmann, Matthias A.

doi:10.1007/s00203-005-0021-4

Cited by 34 publications

(23 citation statements)

References 32 publications

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“…This is the main reason why an increase in pressure is hazardous, even deadly to the cells [25]. It is also a fact that the ribosomal protein genes are among those highly expressed under these conditions [26]. It appears reasonable to assume that accelerated accumulation of radical substitutions in the genes for ribosomal functions may be associated with rearrangement of the ribosomal complex to stabilize it under normal pressure arisen after divergence from the deep-sea ancestors.…”

Section: Resultsmentioning

confidence: 99%

Molecular evolution of the hyperthermophilic archaea of the Pyrococcus genus: analysis of adaptation to different environmental conditions

2009

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BackgroundProkaryotic microorganisms are able to survive and proliferate in severe environmental conditions. The increasing number of complete sequences of prokaryotic genomes has provided the basis for studying the molecular mechanisms of their adaptation at the genomic level. We apply here a computer-based approach to compare the genomes and proteomes from P. furiosus, P. horikoshii, and P. abyssi to identify features of their molecular evolution related to adaptation strategy to diverse environmental conditions.ResultsPhylogenetic analysis of rRNA genes from 26 Pyrococcus strains suggested that the divergence of P. furiosus, P. horikoshii and P. abyssi might have occurred from ancestral deep-sea organisms. It was demonstrated that the function of genes that have been subject to positive Darwinian selection is closely related to abiotic and biotic conditions to which archaea managed to become adapted. Divergence of the P. furiosus archaea might have been due to loss of some genes involved in cell motility or signal transduction, and/or to evolution under positive selection of the genes for translation machinery. In the course of P. horikoshii divergence, positive selection was found to operate mainly on the transcription machinery; divergence of P. abyssi was related with positive selection for the genes mainly involved in inorganic ion transport. Analysis of radical amino acid replacement rate in evolving P. furiosus, P. horikoshii and P. abyssi showed that the fixation rate was higher for radical substitutions relative to the volume of amino acid side-chain.ConclusionsThe current results give due credit to the important role of hydrostatic pressure as a cause of variability in the P. furiosus, P. horikoshii and P. abyssi genomes evolving in different habitats. Nevertheless, adaptation to pressure does not appear to be the sole factor ensuring adaptation to environment. For example, at the stage of the divergence of P. horikoshii and P. abyssi, an essential evolutionary role may be assigned to changes in the trophic chain, namely, acquisition of a consumer status at a high (P. horikoshii) or low level (P. abyssi).

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Section: Resultsmentioning

confidence: 99%

Molecular evolution of the hyperthermophilic archaea of the Pyrococcus genus: analysis of adaptation to different environmental conditions

2009

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“…Similarly, one might predict that a pressure-induced decrease in ppGpp levels would result in the production of increased amounts of ribosomal proteins L7/L12, S6, the elongation factor EF-G, and cold shock proteins (46). Most of these markers can in fact be detected by proteomic analysis of E. coli subjected to a sudden pressure upshift (93) and microarray analysis of high-pressureshocked Lactobacillus sanfranciscensis (71).…”

Section: (I) Previously Isolated Genes (Classes T and I) (Strains Fl4mentioning

confidence: 99%

Large-Scale Transposon Mutagenesis ofPhotobacterium profundumSS9 Reveals New Genetic Loci Important for Growth at Low Temperature and High Pressure

et al. 2008

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Microorganisms adapted to piezopsychrophilic growth dominate the majority of the biosphere that is at relatively constant low temperatures and high pressures, but the genetic bases for the adaptations are largely unknown. Here we report the use of transposon mutagenesis with the deep-sea bacterium Photobacterium profundum strain SS9 to isolate dozens of mutant strains whose growth is impaired at low temperature and/or whose growth is altered as a function of hydrostatic pressure. In many cases the gene mutation-growth phenotype relationship was verified by complementation analysis. The largest fraction of loci associated with temperature sensitivity were involved in the biosynthesis of the cell envelope, in particular the biosynthesis of extracellular polysaccharide. The largest fraction of loci associated with pressure sensitivity were involved in chromosomal structure and function. Genes for ribosome assembly and function were found to be important for both low-temperature and high-pressure growth. Likewise, both adaptation to temperature and adaptation to pressure were affected by mutations in a number of sensory and regulatory loci, suggesting the importance of signal transduction mechanisms in adaptation to either physical parameter. These analyses were the first global analyses of genes conditionally required for low-temperature or high-pressure growth in a deep-sea microorganism.

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“…Similarly L. sanfranciscensis cells were subjected to a 45 MPa pressure shock for 30 min and E. coli cells were grown at 30 MPa. Microarray analysis of the known or predicted genes of yeast and bacteria showed that between 5% and 10% were affected by hydrostatic pressure treatment (Fernandes et al, 2004;Ishii et al, 2005;Iwahashi et al, 2005;Pavlovic et al, 2005) with similar profiles observed after growth at high pressure or HHP shock. Since the complete genome of L. sanfranciscensis is not yet known, the study was conducted using shot-gun microarray analysis (Pavlovic et al, 2005).…”

Section: Pressure Effect On Information Storage and Utilizationmentioning

confidence: 71%

Changes in Transcription and Protein Profile Induced by High Hydrostatic Pressure Treatment in Micro-Organisms

Palhano¹,

Foguel²,

Lindsey³

et al. 2008

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Research on the effects of exposure of micro-organisms to high hydrostatic pressure (HHP) has grown in the last decade. The main foci have been to understand adaptation to life in the deep ocean and the HHP stress response. Amongst other effects, HHP interferes with the cellular membrane structure, increasing the order of lipid molecules especially in the vicinity of proteins, leading to decreased membrane fluidity. Protein structure may also be affected by pressure, disturbing polymerization, folding and the activity. HHP also inhibits protein synthesis, one of the most piezosensitive cellular functions. Ribosome disassembly contributes to this inhibition. RNA synthesis is maintained at pressures at which DNA and protein synthesis are completely inhibited. A number of these responses overlap with responses to other forms of stress, particularly cold stress. Microarray analysis of micro-organisms has identified numerous genes upregulated after exposure to high pressure. However, many of these genes code for proteins of unknown function. Corresponding proteomic analyses detect very few proteins synthesized as a result of such exposure. The review high lights the need for future research to understand the complex pathways involved in the response to HHP.

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Transcriptional response reveals translation machinery as target for high pressure in Lactobacillus sanfranciscensis

Cited by 34 publications

References 32 publications

Molecular evolution of the hyperthermophilic archaea of the Pyrococcus genus: analysis of adaptation to different environmental conditions

Molecular evolution of the hyperthermophilic archaea of the Pyrococcus genus: analysis of adaptation to different environmental conditions

Large-Scale Transposon Mutagenesis ofPhotobacterium profundumSS9 Reveals New Genetic Loci Important for Growth at Low Temperature and High Pressure

Changes in Transcription and Protein Profile Induced by High Hydrostatic Pressure Treatment in Micro-Organisms

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