Transcriptional response of the xerotolerant <i>Arthrobacter</i> sp. Helios strain to PEG-induced drought stress

Hernández‐Fernández, Gabriel; Galán, Beatriz; Carmona, Manuel; Castro, Laura; Garcı́a, José Luis

doi:10.1101/2022.07.26.501551

Cited by 2 publications

(1 citation statement)

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“…Helios or Exiguobacterium sp. Helios whose polyextremophilic characteristics suggest a high biotechnological potential (Castillo-López et al, 2021;Hernández-Fernández et al, 2022). In this work, we provide the first report and characterization of solar panel culturable cyanobacterial isolates that belonging to the orders Chroococcidiopsidales and Synechococcales, the latter including members of three genera-Leptolyngbya, Oculatella, and Myxacorys.…”

Section: Discussionmentioning

confidence: 91%

First characterization of cultivable extremophile Chroococcidiopsis isolates from a solar panel

Baldanta

Arnal²,

Blanco-Rivero³

et al. 2023

Front. Microbiol.

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IntroductionMicroorganisms colonize a wide range of natural and artificial environments. Even though most of them are unculturable in laboratory conditions, some ecosystems are ideal niches for bioprospecting extremophiles with unique properties. Up today, there are few reports concerning microbial communities found on solar panels, a widespread, artificial, extreme habitat. Microorganisms found in this habitat belong to drought-, heat- and radiation-adapted genera, including fungi, bacteria, and cyanobacteria.MethodsHere we isolated and identified several cyanobacteria from a solar panel. Then, some strains isolated were characterizated for their resistance to desiccation, UV-C exposition, and their growth on a range of temperature, pH, NaCl concentration or diverse carbon and nitrogen sources. Finally, gene transfer to these isolates was evaluated using several SEVA plasmids with different replicons to assess their potential in biotechnological applications.Results and discussionThis study presents the first identification and characterization of cultivable extremophile cyanobacteria from a solar panel in Valencia, Spain. The isolates are members of the genera Chroococcidiopsis, Leptolyngbya, Myxacorys, and Oculatella all genera with species commonly isolated from deserts and arid regions. Four of the isolates were selected, all of them Chroococcidiopsis, and characterized. Our results showed that all Chroococcidiopsis isolates chosen were resistant up to a year of desiccation, viable after exposition to high doses of UV-C, and capable of being transformed. Our findings revealed that a solar panel is a useful ecological niche in searching for extremophilic cyanobacteria to further study the desiccation and UV-tolerance mechanisms. We conclude that these cyanobacteria can be modified and exploited as candidates for biotechnological purposes, including astrobiology applications.

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Section: Discussionmentioning

confidence: 91%

First characterization of cultivable extremophile Chroococcidiopsis isolates from a solar panel

Baldanta

Arnal²,

Blanco-Rivero³

et al. 2023

Front. Microbiol.

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The Microbiome of Fertilization-Stage Maize Silks (Style) Encodes Genes and Expresses Traits That Potentially Promote Survival in Pollen/Style Niches and Host Reproduction

Thompson,

Raizada

2024

Microorganisms

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Within flowers, the style channel receives pollen and transmits male gametes inside elongating pollen tubes to ovules. The styles of maize/corn are called silks. Fertilization-stage silks possess complex microbiomes, which may partially derive from pollen. These microbiomes lack functional analysis. We hypothesize that fertilization-stage silk microbiomes promote host fertilization to ensure their own vertical transmission. We further hypothesize that these microbes encode traits to survive stresses within the silk (water/nitrogen limitation) and pollen (dehydration/aluminum) habitats. Here, bacteria cultured from fertilization-stage silks of 14 North American maize genotypes underwent genome mining and functional testing, which revealed osmoprotection, nitrogen-fixation, and aluminum-tolerance traits. Bacteria contained auxin biosynthesis genes, and testing confirmed indole compound secretion, which is relevant, since pollen delivers auxin to silks to stimulate egg cell maturation. Some isolates encoded biosynthetic/transport compounds known to regulate pollen tube guidance/growth. The isolates encoded ACC deaminase, which degrades the precursor for ethylene that otherwise accelerates silk senescence. The findings suggest that members of the microbiome of fertilization-stage silks encode adaptations to survive the stress conditions of silk/pollen and have the potential to express signaling compounds known to impact reproduction. Overall, whereas these microbial traits have traditionally been assumed to primarily promote vegetative plant growth, this study proposes they may also play selfish roles during host reproduction.

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Transcriptional response of the xerotolerant Arthrobacter sp. Helios strain to PEG-induced drought stress

Cited by 2 publications

References 97 publications

First characterization of cultivable extremophile Chroococcidiopsis isolates from a solar panel

First characterization of cultivable extremophile Chroococcidiopsis isolates from a solar panel

The Microbiome of Fertilization-Stage Maize Silks (Style) Encodes Genes and Expresses Traits That Potentially Promote Survival in Pollen/Style Niches and Host Reproduction

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