Transcriptional response of grapevine to infection with the fungal pathogen Lasiodiplodia theobromae

Abstract

Botryosphaeria dieback on the grapevine is caused by Botryosphaeriaceae fungi, which threatens the yield and quality of grapes. At present, chemical control strategies are often observed to be ineffective in controlling the disease worldwide. Improving our understanding of the molecular mechanisms that confer resistance to pathogens would facilitate the development of more pathogen-tolerant grape varieties. Here, we used RNA sequencing analysis to profile the transcriptome of grapevine green shoots infected with Lasiodiplodia theobromae over a time course of 4, 8 and 12 hours post inoculation. A total of 5181 genes were identified as differentially expressed genes (DEGs), and DEGs were more abundant over time. Further analysis revealed that many of these DEGs are involved in plant-pathogen interactions, hormone signal transduction and phenylpropanoid biosynthesis pathways, suggesting that innate immunity, phytohormone signaling and many phenylpropanoid compounds, which constitute a complex defense network in plants, are involved in the response of grapevine against to L . theobromae infection. This study provides novel insights into the molecular mechanisms of plant–pathogen interactions that will be valuable for the genetic improvement of grapevines.

The Biocontrol Root-Oomycete, Pythium Oligandrum, Triggers Grapevine Resistance and Shifts in the Transcriptome of the Trunk Pathogenic Fungus, Phaeomoniella Chlamydospora

“…In all treatments, expression levels of ISR-related genes, i.e., phenylpropanoid biosynthesis, signaling hormone pathways and transcription factors, were more expressed at 0-dpi than those observed at 14-dpi. These results are in accordance with previous studies showing that grapevine defense genes are, generally, induced a few hours following biotic stress applications [25][26][27][28][29] as it is the case in our experiment where 0-dpi corresponded to 2 h after plant inoculation with the pathogen, P. chlamydospora.…”

Transcriptomic analysis of Dubas bug (Ommatissus lybicus Bergevin) infestation to Date Palm

“…Our results showed 38 upregulated DEGs that were associated with phenyl propanoid biosynthesis pathway during DB infection. Similar results were reported by Zhang et al 68 in grapevine under fungal (Lasiodiplodia theobromae) attack. Accumulation of these metabolites in infected samples suggest active contribution during plant-defense responses in date palm during DB infection.…”

“…Other than auxin, GA are regulated through DELLA protein family 69,70 that also help to maintain SA and JA homeostasis [65][66][67] during infection process. Our findings are in synergy to Zhang et al 68 that these are upregulated during fungal infection in grapes plants. Since, SA is involved in systemic acquired resistance (SAR) and regulation of plant defense responses 69 , we noted activation of PR1 genes.…”

“…The results of the current study were consistent with previously reported study 82 . Similarly, increased expression of 4CL and PAL genes in date palm are in synergy to grapevine after infection 68 . Moreover, similar results were recorded in Arabidopsis 83 .…”

Genomic Evidences Support an Independent History of Grapevine Domestication in the Levant

“…Outlier QTRs that were detected exclusively in the Eurasian sativa group harbored the anthocyanin synthesis genes (MYBA1 and MYBA3) on chromosome 2 (32,33), the pathogen response gene (ACD6) on chromosome 16 (35), and the disease response genes (RPM1) on chromosome 7 which were described to be up-regulated in response to pathogen infection in grapevine (36).…”