Transcriptional repression of miR-200 family members by Nanog in colon cancer cells induces epithelial–mesenchymal transition (EMT)

Pan, Qiong; Meng, Linkun; Ye, Jun; Wei, Xiaolong; Shang, Yangyang; Yin, Tian; He, Yonghong; Peng, Zhihong; Chen, Lei; Chen, Wensheng; Bian, Xiu‐Wu; Wang, Rongquan

doi:10.1016/j.canlet.2017.01.039

Cited by 54 publications

(39 citation statements)

References 33 publications

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“…12 As expected, hypomethylation of the promoter regions, resulted in the re-expression of epithelial markers (E-Cadherin, Lethal giant larvae 2). 24 The identification and manipulation of transcription factors as an adjunct to promoting an epithelial phenotype could be investigated as it may have a therapeutic potential. 21 Fessler et al demonstrated epigenetic methylation of the miR-200 family promoter regions is associated with miR-200 family repression and a mesenchymal phenotype in a group of clinical samples.…”

Section: Resultsmentioning

confidence: 99%

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The role of the miR‐200 family in epithelial–mesenchymal transition in colorectal cancer: a systematic review

O’Brien

Carter

Burton

et al. 2018

Intl Journal of Cancer

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Colorectal cancer (CRC) is associated with significant morbidity and mortality as many patients are diagnosed with advanced stage disease. MicroRNAs are small, noncoding RNA molecules that have a major role in gene expression regulation and are dysregulated in CRC. The miR-200 family is involved in epithelial-mesenchymal transition (EMT). This systematic review describes the roles of the miR-200 family in EMT in CRC. A search of electronic databases (PubMed and Embase) was conducted between January 2000 and July 2017. Both in vitro and human studies reporting on the miR-200 family and CRC were included. Studies describing molecular pathways and the role of the miR-200 family in the diagnostic and therapeutic management of CRC were analyzed. Thirty-four studies (22 in vitro and 18 human studies) were included. miR-200 family expression is regulated epigenetically and via transcriptional factor regulation. In vitro studies show that transfection of miR-200 family members into chemo-resistant colon cancer cell lines results in improved chemo-sensitivity and epithelial phenotype restoration. There is intra-tumoral variability in the tissue expression of miR-200 family members with decreased expression at the invasive front. Clinical studies in CRC patients have shown decreased primary tumor tissue expression of miR-429, miR-200a and miR-200c may be associated with worse survival. Conversely, increased blood levels of miR-141, miR-200a and miR-200c may be associated with worse outcomes. The miR-200 family has a central role in EMT. The miR200 family has potential for both prognostic and therapeutic management of CRC.

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Section: Resultsmentioning

confidence: 99%

“…Pan et al demonstrated that it bound directly to the separate miR-200 promoter regions, repressing their transcription and resulting in a mesenchymal phenotype. 24 The identification and manipulation of transcription factors as an adjunct to promoting an epithelial phenotype could be investigated as it may have a therapeutic potential.…”

Section: Resultsmentioning

confidence: 99%

The role of the miR‐200 family in epithelial–mesenchymal transition in colorectal cancer: a systematic review

O’Brien

Carter

Burton

et al. 2018

Intl Journal of Cancer

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“…Guo concluded the multidimensional communication of microRNAs and lncRNAs in lung cancer and mentioned that miRNA-lncRNA pathways may have the common end point of EMT. For example, the miR-200 family were found could inhibit EMT by targeting mRNA of ZEB1 and ZEB2 who are the repressors of E-cadherin [22]. Respectively, Li et al and Zhang et al found that the lncRNA MALTA1 and lncRNA H19 with their targets miR-204 and miR-484 played an active role in the process of EMT in lung cancer [23].…”

Section: Introductionmentioning

confidence: 99%

The long noncoding RNA linc00858 promotes progress of lung cancer through miR-3182/MMP2 axis

Xue

Shi

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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In previous studies, numerous dysregulated lncRNAs were identified using RNA-sequencing. Lung cancer is the most common malignant tumour worldwide and the second leading cause in cancer. The aim of this study is to investigate the function and mechanism of lincRNA00858 in the lung cancer. RT-PCR was used to detect the expression of lincRNA00858. Proliferation, invasion, and epithelial-mesenchymal transition (EMT) were examined by CCK8, transwell assay and western blot to evaluate the function of linc00858. Dual-luciferase reporter assay was used to identified the potential target of linc00858. Overexpression of linc00858 significantly promoted cell proliferation, invasion. We also found that linc00858 facilitated the EMT process. Dual-luciferase reporter assay revealed that linc00858 can bind with miR-3182 directly. Linc00858 can negatively regulate the expression of miR-3182. Further experiments demonstrated that MMP2 was the direct target of miR-3182. Rescue experiments revealed that linc00858 functioned through miR-3182/MMP2 axis. Taken together, we verified the role of an unknown linc00858 in lung cancer and provided its mechanism. Mechanistically, linc00858 acted as a competitive endogenous RNA to sponged miR-3182 and regulate MMP2 in lung cancer. Our study provided new clues for understanding the mechanism of lung cancer.

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“…Essa característica permite que os miRNAs participem dos mais diversos processos celulares e biológicos, regulando diferentes alvos e vias de sinalização, sejam eles em situações patológicas ou não-patológicas. Na literatura científica, são encontrados diversos trabalhos associando estes pequenos RNAs reguladores à doenças neurodegenerativas (HE et al, 2017;HÉBERT;DE STROOPER, 2009;KIM et al, 2007;NELSON;RAJEEV, 2008;RIANCHO et al, 2017;TAGLIAFIERRO et al, 2017), musculares e cardíacas (BITTEL et al, 2014;CARÈ et al, 2007;EISENBERG et al, 2007); e nos mais diferentes tipos de câncer (HAYES; PERUZZI; LAWLER, 2014;JIA et al, 2017;KIM et al, 2017;PAN et al, 2017;ROSENFELD et al, 2008;ZHENG et al, 2017;ZHOU et al, 2017). Nessas situações, os miRNA podem aparecer como figuras importantes na patogênese, manutenção e/ou progressão da doença, biomarcadores para o diagnóstico e prognóstico e como ferramentas para o tratamento, sejam como agentes, alvos ou para elucidar os mecanismos potencialmente moduláveis.…”

Section: Figura 2 Possíveis Aplicações Práticas Das Células-tronco Punclassified

“…Diversos são os mecanismos envolvidos no controle da EMT, como fatores de transcrição, moduladores epigenéticos e miRNAs (CICCHINI et al, 2015;DE CRAENE;BERX, 2013;GALVÁN et al, 2015;HUA et al, 2011;PAN et al, 2017;ZHOU et al, 2015). Estes reguladores atuam, por exemplo, induzindo o rearranjo do citoesqueleto de actina, alterações na composição dos receptores de matriz extracelular, levando assim à perda da adesão focal e, consequentemente, da polaridade apical-basal das células epiteliais (LAMOUILLE; XU; DERYNCK, 2014;THIERY et al, 2009;TOJKANDER;GATEVA;LAPPALAINEN, 2012).…”

Section: Regulação Da Transição Epitelial-mesenquimal Por Mirnas Capaunclassified

Identificação de vias moduladas por microRNAs na diferenciação celular e manutenção da pluripotência em células humanas

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Transcriptional repression of miR-200 family members by Nanog in colon cancer cells induces epithelial–mesenchymal transition (EMT)

Cited by 54 publications

References 33 publications

The role of the miR‐200 family in epithelial–mesenchymal transition in colorectal cancer: a systematic review

The role of the miR‐200 family in epithelial–mesenchymal transition in colorectal cancer: a systematic review

The long noncoding RNA linc00858 promotes progress of lung cancer through miR-3182/MMP2 axis

Identificação de vias moduladas por microRNAs na diferenciação celular e manutenção da pluripotência em células humanas

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