Transcriptional repression due to high levels of Wingless signalling

Yu, Xiang; Riese, Jens; Eresh, Salih; Bienz, Mariann

doi:10.1093/emboj/17.23.7021

Cited by 34 publications

(54 citation statements)

References 69 publications

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“…Four copies of a TCF binding site, taken from a Wntregulated midgut enhancer of the Ultrabithorax (Ubx) gene, were placed upstream of a minimal TATAcontaining promoter driving lacZ. This binding site is necessary for Wnt-mediated activation of the Ubx enhancer in vivo (Riese et al, 1997;Yu et al, 1998). However, transgenic flies carrying the multimerized TCF site reporter construct showed no lacZ expression in Wnt-responding cells, even upon overexpression of the Wingless ligand (Riese et al, 1997).…”

Section: Transgenic Tcf Reporters: a Brief Historymentioning

confidence: 99%

“…This transgene contains lacZ driven by a minimal TATA-containing promoter, downstream of four copies of natural TCF binding sites taken from a Wnt-regulated enhancer of the Ultrabithorax gene (Yu et al, 1998;see Table 1). Like the Riese reporter, my 4 Â TCF-lacZ construct is completely inactive in Wnt-responsive tissues, throughout embryonic development and in larval imaginal discs ( Figure 1).…”

Section: Hits and Misses Among Tcf Reportersmentioning

confidence: 99%

“…Interestingly, both of the non-responding fly reporters use natural TCF binding sites that deviate from this 'optimal' sequence (Table 1). In addition, the number of TCF sites in reporter transgenes is very often higher than in native Wnt/TCF target enhancers, many of which contain only one or two TCF sites (e.g., Giese et al, 1995;Haynes et al, 1996;Yu et al, 1998;Shtutman et al, 1999;Arnold et al, 2000;Halfon et al, 2000;Yang et al, 2000;Maduro et al, 2005). Perhaps placing a large number of close-packed, optimal-affinity binding sites immediately upstream of a promoter is sufficient to generate a transcriptional response not seen in most native TCF-regulated enhancers.…”

Section: Why Do Vertebrate Tcf Reporters Work?mentioning

confidence: 99%

“…Transgenic Wnt/TCF reporters S Barolo cAMP response element (CRE) (Robertson et al, 1995), which may contribute to activation in some contexts. TCF sites and CREs act cooperatively on certain Wnt target genes (Hernandez-Munain et al, 1998;Yu et al, 1998;Spychala et al, 1999;Pradeep et al, 2004). Moreover, Wnts have recently been shown to activate transcription directly via CREs (Chen et al, 2005).…”

Section: Why Do Vertebrate Tcf Reporters Work?mentioning

confidence: 99%

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Transgenic Wnt/TCF pathway reporters: all you need is Lef?

2006

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Section: Transgenic Tcf Reporters: a Brief Historymentioning

confidence: 99%

Section: Hits and Misses Among Tcf Reportersmentioning

confidence: 99%

Section: Why Do Vertebrate Tcf Reporters Work?mentioning

confidence: 99%

Section: Why Do Vertebrate Tcf Reporters Work?mentioning

confidence: 99%

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Transgenic Wnt/TCF pathway reporters: all you need is Lef?

2006

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“…The Ubx midgut enhancer drives Ubx expression in parasegment (ps) 7 of the embryonic midgut (Thuringer et al, 1993). Two elements, one which is Wg responsive (the WRS) and another Dpp responsive (the DRS) function synergistically to activate Ubx expression in ps 7 expression (Yu et al, 1998). In the adjacent ps8, however, Brk binds to the DRS and blocks the activity of the WRS (Saller et al, 2002).…”

Section: Integration Of Dpp and Other Signalsmentioning

confidence: 99%

Opposing inputs by Hedgehog and Brinker define a stripe ofhairyexpression in theDrosophilaleg imaginal disc

et al. 2004

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Heads or Tails? Amphioxus and the Evolution of Anterior–Posterior Patterning in Deuterostomes

Holland

2002

Developmental Biology

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In Xenopus, the canonical Wnt-signaling pathway acting through beta-catenin functions both in establishing the dorso-ventral axis and in patterning the anterior-posterior axis. This pathway also acts in patterning the animal-vegetal axis in sea urchins. However, because sea urchin development is typically indirect, and adult sea urchins have pentamerous symmetry and lack a longitudinal nerve cord, it has not been clear how the roles of the canonical Wnt-signaling pathway in axial patterning in sea urchins and vertebrates are evolutionarily related. The developmental expression patterns of Notch, brachyury, caudal, and eight Wnt genes have now been determined for the invertebrate chordate Amphioxus, which, like sea urchins, has an early embryo that gastrulates by invagination, but like vertebrates, has a later embryo with a dorsal hollow nerve cord that elongates posteriorly from a tail bud. Comparisons of Amphioxus with other deuterostomes suggest that patterning of the ancestral deuterostome embryo along its anterior-posterior axis during the late blastula and subsequent stages involved a posterior signaling center including Wnts, Notch, and transcription factors such as brachyury and caudal. In tunicate embryos, in which cell numbers are reduced and cell fates largely determined during cleavage stages, only vestiges of this signaling center are still apparent; these include localization of Wnt-5 mRNA to the posterior cytoplasm shortly after fertilization and localization of beta-catenin to vegetal nuclei during cleavage stages. Neither in tunicates nor in Amphioxus is there any evidence that the canonical Wnt-signaling pathway functions in establishment of the dorso-ventral axis. Thus, roles for Wnt-signaling in dorso-ventral patterning of embryos may be a vertebrate innovation that arose in connection with the evolution of yolky eggs and gastrulation by extensive involution.

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Transcriptional repression due to high levels of Wingless signalling

Cited by 34 publications

References 69 publications

Transgenic Wnt/TCF pathway reporters: all you need is Lef?

Transgenic Wnt/TCF pathway reporters: all you need is Lef?

Opposing inputs by Hedgehog and Brinker define a stripe ofhairyexpression in theDrosophilaleg imaginal disc

Heads or Tails? Amphioxus and the Evolution of Anterior–Posterior Patterning in Deuterostomes

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