Transcriptional Regulatory Cascades in Runx2-Dependent Bone Development

Liu, Tongming; Lee, Eng Hin

doi:10.1089/ten.teb.2012.0527

Cited by 275 publications

(208 citation statements)

References 97 publications

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“…RUNX2 is expressed in cells prior to the formation of the skeleton, as early as E10.5, at which stage cells still have the capacity to differentiate into osteoblasts or chondrocytes [36-38]. RUNX2 is a master transcription factor for osteoblast differentiation, matrix production and mineralization during bone formation [38].…”

Section: Discussionmentioning

confidence: 99%

“…RUNX2 is a master transcription factor for osteoblast differentiation, matrix production and mineralization during bone formation [38]. RUNX2 regulates OSX, which is a zinc-finger-containing transcription factor expressed in osteoblasts that is essential for osteoblast differentiation and bone formation, [39] and ALPL, which is translated into a ubiquitous cellular protein that is an early indicator of cellular activity and differentiation [40, 41].…”

Section: Discussionmentioning

confidence: 99%

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Rapid Induction of Osteogenic Markers in Mesenchymal Stem Cells by Adipose-Derived Stromal Vascular Fraction Cells

Choi

Shin

Lee

et al. 2017

Cell Physiol Biochem

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Background/Aims: Stromal vascular fraction (SVF) cells are a mixed cell population, and their regenerative capacity has been validated in various therapeutic models. The purpose of this study was to investigate the regenerative mechanisms utilized by implanted SVF cells. Using an in vitro co-culture system, we sought to determine whether SVF implantation into impaired tissue affects endogenous mesenchymal stem cell (MSC) differentiation; MSCs can differentiate into a variety of cell types, and they have a strong regenerative capacity despite their low numbers in impaired tissue. Methods: Adipose-derived SVF cells obtained from four donors were co-cultured with bone marrow-derived MSCs, and the differential expression of osteogenic markers and osteogenic differentiation inducers over time was analyzed in mono-cultured MSCs and MSCs co-cultured with SVF cells. Results: The co-cultivation of MSCs with SVF cells significantly and mutually induced the expression of osteogenic-specific markers via paracrine and/or autocrine regulation but did not induce adipocyte, chondrocyte or myoblast marker expression. More surprisingly, subsequent osteogenesis and/or comparable effects were rapidly induced within 48 h. Conclusion: To the best of our knowledge, this is the first study in which osteogenesis and/or comparable effects were rapidly induced in bone marrow-derived MSCs and adipose-derived SVF cells through co-cultivation. Our findings suggest that the positive effects of SVF implantation into impaired bone may be attributed to the rapid induction of MSC osteogenesis, and the transplantation of co-cultured and preconditioned SVF cells and/or MSCs may be more effective than the transplantation of untreated cells for the treatment of bone defects.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Rapid Induction of Osteogenic Markers in Mesenchymal Stem Cells by Adipose-Derived Stromal Vascular Fraction Cells

Choi

Shin

Lee

et al. 2017

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…This remodeling of the vasculature can extend to the point of acquiring some functional characteristics of bone, offering the perspective that VC occurs in a manner similar to bone development 48, 60, 61, 62. Briefly, osteogenesis involves formation of mesenchymal cell condensations, which differentiate into bone‐forming cells for intramembranous or endochondral ossification 63, 64. Direct differentiation of mesenchymal cells to osteoblasts in intramembranous (IM) bone development allows deposition of mineralization‐ready bone matrix, whereas EC ossification first requires the deposition of a cartilage template by chondrocytes, on which osteoblasts will elaborate to form bone 65.…”

Section: Vascular Calcificationmentioning

confidence: 99%

Atherosclerotic Calcification: Wnt Is the Hint

Albanese

Khan

Barratt

et al. 2018

JAHA

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“…Local factors, cytokines, chemokines, transcription factors, mechanical forces, and bone turnover hormones bring about the tight regulation of bone homeostasis. The expression of a key osteoblast differentiation transcription factor, Runt-related transcription factor 2 (Runx2) is essential for Mesenchymal stem cells differentiation into the osteoblastic lineage (Liu and Lee, 2013). Runx2 is also called as core-binding factor subunit alpha-1(CBF-alpha-1) protein encoded by the Runx2 gene.…”

Section: Mechanism Of Action Of Marine Natural Products On Bone Metabmentioning

confidence: 99%

Marine Natural Products: New Avenue in Treatment of Osteoporosis

Chaugule¹,

Indap²,

Chiplunkar

2017

Front. Mar. Sci.

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Bone metabolism is a physiological process that maintains the skeletal integrity and bone functions. Skeletal integrity is always balanced by two key cell types-bone resorbing osteoclasts and bone-forming osteoblasts. Imbalance between generation and function of osteoclasts and osteoblasts often leads to pathological conditions such as osteoporosis, osteopetrosis, Paget's disease. Osteoporosis is one of the most common age-related diseases characterized by decreased bone mineral density and microarchitectural deterioration. Current therapies are indeed effective in preventing bone loss but are also followed by side effects. Since many years, marine organisms have been considered as a good source of bioactive molecules or compounds with potential pharmaceutical properties. Marine Natural Products (MNPs) derived from various marine resources such as marine cyanobacteria, dinoflagellates, algae, sponges, soft corals, molluscs, fishes, and mangroves had shown profound effect on bone metabolism through inhibiting osteoclastogenesis and up-regulating osteoblastogenesis via modulating RANK/RANKL/OPG pathway. Amongst the pre-clinically investigated MNPs for management of osteoporosis, very few are under phase I clinical trials. This review discusses the currently available pharmacological drugs and there major health concern in osteoporosis treatment. It further gives an insight into various marine resources and marine-derived bioactive products, depicting their mechanism of action, functional role, and how these can be exploited for the treatment of osteoporosis.

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Transcriptional Regulatory Cascades in Runx2-Dependent Bone Development

Cited by 275 publications

References 97 publications

Rapid Induction of Osteogenic Markers in Mesenchymal Stem Cells by Adipose-Derived Stromal Vascular Fraction Cells

Rapid Induction of Osteogenic Markers in Mesenchymal Stem Cells by Adipose-Derived Stromal Vascular Fraction Cells

Atherosclerotic Calcification: Wnt Is the Hint

Marine Natural Products: New Avenue in Treatment of Osteoporosis

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