Transcriptional regulator PrqR plays a negative role in glucose metabolism and oxidative stress acclimation in Synechocystis sp. PCC 6803

Khan, Md. Rezaul Islam; Wang, Yushu; Afrin, Shajia; Wang, Bing; Liu, Yumin; Zhang, Xiaoqing; Chen, Lei; Zhang, Weiwen; He, Lin; Ma, Gang

doi:10.1038/srep32507

Cited by 11 publications

(9 citation statements)

References 60 publications

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“…Hence, it is tempting to speculate that PrqR might be involved in the salt-stimulated expression of ggpS and other genes in this putative regulon. PrqR was reported as repressor for genes involved in glucose metabolism and oxidative stress acclimation [ 39 ]. The LexA protein, which is not changed on protein level but became significantly decreased on RNA level (Additional file 3 : Fig.…”

Section: Resultsmentioning

confidence: 99%

Integrative analysis of the salt stress response in cyanobacteria

et al. 2021

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Microorganisms evolved specific acclimation strategies to thrive in environments of high or fluctuating salinities. Here, salt acclimation in the model cyanobacterium Synechocystis sp. PCC 6803 was analyzed by integrating transcriptomic, proteomic and metabolomic data. A dynamic reorganization of the transcriptome occurred during the first hours after salt shock, e.g. involving the upregulation of genes to activate compatible solute biochemistry balancing osmotic pressure. The massive accumulation of glucosylglycerol then had a measurable impact on the overall carbon and nitrogen metabolism. In addition, we observed the coordinated induction of putative regulatory RNAs and of several proteins known for their involvement in other stress responses. Overall, salt-induced changes in the proteome and transcriptome showed good correlations, especially among the stably up-regulated proteins and their transcripts. We define an extended salt stimulon comprising proteins directly or indirectly related to compatible solute metabolism, ion and water movements, and a distinct set of regulatory RNAs involved in post-transcriptional regulation. Our comprehensive data set provides the basis for engineering cyanobacterial salt tolerance and to further understand its regulation.

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Section: Resultsmentioning

confidence: 99%

Integrative analysis of the salt stress response in cyanobacteria

et al. 2021

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“…Altered levels of the sigma factor sigE (sll1689), the sugar catabolism regulator Rre37 (sll1330), the negative regulator of glucose metabolism and oxidative stress acclimation PrqR (slr0895) and also PmgR1 (ncr0700) suggest an interesting link between CrhR and sugar/energy/heterotrophic metabolism, which is the major functional target category of these regulators [43][44][45][46][47]. PmgR1 (ncr0700), the most strongly induced sRNA in crhR TR , is involved in the switch to photomixotrophic growth and the regulation of glycogen metabolism [48].…”

Section: Altered Mrna Expression Correlates With Altered Cellular Mormentioning

confidence: 99%

Inactivation of the RNA helicase CrhR impacts a specific subset of the transcriptome in the cyanobacterium Synechocystis sp. PCC 6803

et al. 2019

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DEAD-box RNA-helicases catalyze the reorganization of structured RNAs and the formation of RNP complexes. The cyanobacterium Synechocystis sp. PCC 6803 encodes a single DEAD-box RNA helicase, CrhR (Slr0083), whose expression is regulated by abiotic stresses that alter the redox potential of the photosynthetic electron transport chain, including temperature downshift. Despite its proposed effect on RNA metabolism and its known relevance in cold-stress adaptation, the reported impact of a CrhR knockout on the cold adaption of the transcriptome only identified eight affected genes. Here, we utilized a custom designed microarray to assess the impact of the absence of CrhR RNA helicase activity on the transcriptome, independent of cold stress. CrhR truncation impacts an RNA subset comprising~10% of the ncRNA and alsõ 10% of the mRNA transcripts. While equal numbers of mRNAs showed increased as well as decreased abundance, more than 90% of the ncRNAs showed enhanced expression in the absence of CrhR, indicative of a negative effect on ncRNA transcription or stability. We further tested the effect of CrhR on the stability of strongly responding RNAs that identify examples of post-transcriptional and transcriptional regulation. The data suggest that CrhR impacts multiple aspects of RNA metabolism in Synechocystis.

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“…Hence, it is tempting to speculate that PrqR might be involved in the salt-stimulated expression of ggpS and other genes in this putative regulon. PrqR was reported as repressor for genes involved in glucose metabolism and oxidative stress acclimation (Khan et al, 2016). The LexA protein, which is not changed on protein level but became significantly decreased on RNA level (Suppl.…”

Section: Resultsmentioning

confidence: 99%

“…S6). It has been recently shown that PrqR is involved in the acclimation to oxidative stress in Synechocystis 6803 (Khan et al, 2016). Salt stress is also inducing oxidative stress in cyanobacterial cells, hence, the finding of the role of PrqR in this stress acclimation process might of secondary importance.…”

Section: Discussionmentioning

confidence: 99%

Integrative analysis of the salt stress response in cyanobacteria

Klaehn

Mikkat

Riediger

et al. 2021

Preprint

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Microorganisms evolved specific acclimation strategies to thrive in environments of high or fluctuating salinities. Here, salt acclimation in the model cyanobacterium Synechocystis sp. PCC 6803 was analyzed by integrating transcriptomic, proteomic and metabolomic data. A dynamic reorganization of the transcriptome and proteome occurred during the first hours after salt shock, e.g. involving the upregulation of genes to activate compatible solute biochemistry balancing osmotic pressure. The massive accumulation of glucosylglycerol then had a measurable impact on the overall carbon and nitrogen metabolism. In addition, we observed the coordinated induction of putative regulatory RNAs and of several proteins known for their involvement in other stress responses. Overall, salt-induced changes in the proteome and transcriptome showed good correlations, especially among the stably up-regulated proteins and their transcripts. We define an extended salt stimulon comprising proteins directly or indirectly related to compatible solute metabolism, ion and water movements, and a distinct set of regulatory RNAs involved in post-transcriptional regulation. Our comprehensive data set provides the basis for engineering cyanobacterial salt tolerance and to further understand its regulation.

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Transcriptional regulator PrqR plays a negative role in glucose metabolism and oxidative stress acclimation in Synechocystis sp. PCC 6803

Cited by 11 publications

References 60 publications

Integrative analysis of the salt stress response in cyanobacteria

Integrative analysis of the salt stress response in cyanobacteria

Inactivation of the RNA helicase CrhR impacts a specific subset of the transcriptome in the cyanobacterium Synechocystis sp. PCC 6803

Integrative analysis of the salt stress response in cyanobacteria

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