Transcriptional regulation of two serum-induced RNAs in mouse fibroblasts: equivalence of one species to B2 repetitive elements.

Edwards, Dylan R.; Parfett, Craig L.J.; Denhardt, David T.

doi:10.1128/mcb.5.11.3280

Cited by 126 publications

(65 citation statements)

References 40 publications

(43 reference statements)

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“…Fluorescence was measured and quantitated using 672A Software 1.2 (Applied Biosystems). Amounts of TGF-P mRNA were normalized against the corresponding amounts of GAPDH mRNA, which is unaffected by TGF-,1 (Edwards et al, 1985;Norgaard et al, 1996). Results are presented as TGF-, -GAPDH ratio.…”

Section: Methodsmentioning

confidence: 99%

Expression of transforming growth factors beta-1, beta 2 and beta 3 in human bladder carcinomas

Eder

Stenzl²,

Hobisch³

et al. 1997

Br J Cancer

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Summary We previously detected elevated transforming growth factor beta-1 (TGF-11) serum levels in patients with invasive bladder carcinomas. In this study, we therefore investigated whether elevated serum levels correlate with enhanced TGF-1 expression in human bladder tumours. mRNA levels of TGF-p1, -12 and -133 were reduced in bladder tumour tissue to 86%, 68% and 56%, respectively, of the levels in normal urothelium. On the other hand, TGF-1l protein levels were found to be higher in superficial tumours (T7-T,) (mean level of 0.153 ng mg-1) and in invasive TJ/3 tumours (mean level of 0.104 ng mg-') compared with normal urothelium (mean level of 0.065 ng mg-').Invasive T4 tumours, however, contained only low amounts of TGF-1l (mean level of 0.02 ng mg-').

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Section: Methodsmentioning

confidence: 99%

Expression of transforming growth factors beta-1, beta 2 and beta 3 in human bladder carcinomas

Eder

Stenzl²,

Hobisch³

et al. 1997

Br J Cancer

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“…Accumulation of collagen, fibronectin, extracellular proteases, and protease inhibitors is modulated by TGF-,, all of which affect differentiation (19,31,33,43,50). To investigate whether excess accumulation of extracellular matrix components limited differentiation in this system, the steady-state level of mRNAs encoding collagen a2(I), fibronectin, TGF-4, and tissue inhibitor of metalloendoprotease were examined by Northern blot analysis.…”

Section: Resultsmentioning

confidence: 99%

Isolation and characterization of a variant myoblast cell line that is temperature sensitive for differentiation.

Akhurst¹,

Flavin²,

Worden³

1988

Mol. Cell. Biol.

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A new variant rat myogenic cell line, ts485, was isolated by subcloning the cell line ts3b2 (H. T. Nguyen, R. M. Medford, and B. Nadal-Ginard, Cell 34:281-293, 1983). Unlike the progenitor cell line, ts485 was thermosensitive for differentiation. Experiments with conditioned medium suggested that diffusible extracellular factors were not involved in dictating the differential phenotypes of ts485 cells cultured at the permissive and nonpermissive temperatures. Temperature shift experiments performed on cultures of ts485 cells indicated that the temperature-sensitive lesion was in a factor active during the growth phase and required to trigger a cascade of events leading to terminal differentiation.Our understanding of the molecular control of gene expression during cellular differentiation is still superficial despite efforts with multiple experimental approaches. At the genomic level, several types of cis-acting DNA sequences which regulate gene expression in a tissue-specific manner have been characterized (14,28,36). trans-Acting, tissue-specific regulatory factors which may interact with such elements have been identified by a combination of molecular (17), biochemical (41), and cell biological approaches (8,22). In most biological systems, complexities and incongruities in the tissue-specific regulation of gene expression suggest a hierarchy of control steps which to date remain unresolved (26,28,36,53).Muscle is an ideal system with which to study molecular differentiation in vitro. Mononucleate myoblasts from primary cultures or established cell lines can be propagated in the undifferentiated state under conditions of high serum and low cell density. After removal of mitogens from the medium, the cells will align, differentiate, and fuse to form multinucleate myotubes. The muscle-specific genes encoding contractile proteins, cell surface receptors, and energygenerating enzymes are coordinately activated during this process (10,23,48). By experimental manipulation, myogenesis has been dissected into sequential stages, beginning with withdrawal from the cell cycle at Gl. This is followed by muscle-specific gene activation, commitment to terminal differentiation, and cell fusion (37, 40). Each step can be studied independently with biological inhibitors of the various processes of myogenesis (3,15,31,39,46).Identification of conditional myoblast mutants temperature sensitive for various stages of myogenesis has allowed further characterization of the sequential events of myogenesis at the morphological and biochemical levels (30, 40). The rat myoblast line ts3b2, in particular, has been extensively studied with respect to its temperature-sensitive phenotype for commitment to terminal differentiation (39, 40). The availability of other temperature-sensitive cell lines defective for various stages of myogenesis would not only provide models to probe the mechanisms regulating differentiation but could also provide the potential to isolate specific regulatory genes by DNA complementation.Here we report the isolat...

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“…Cultures of Swiss 3T3 fibroblasts and second passage mouse embryo fibroblasts (MEF) from 14-16 day Swiss mouse embryos were maintained as described previously (9,14). The mouse epidermal cell line JB6 C122 was obtained from Dr. N. Colburn and was cultured in Eagle's medium containing 8% fetal bovine serum.…”

Section: Materials and Methods Cell Culturementioning

confidence: 99%

“…12,13). We reported some properties of one of these genes, designated 16C8, in an earlier publication (14). Serum, FGF and TPA were each able, after a lag period, to stimulate transcription of the 16C8 gene in quiescent murine cells.…”

Section: Introductionmentioning

confidence: 99%

“…In contrast to these "early" genes, others, typified by 16C8 (14), MEP, (major excreted protein, 18) and pTRl (transin, 19), require protein synthesis for their full induction in subconfluent mouse fibroblast cultures. In this paper, we report the nucleotide sequence 6f a nominally full length cDNA clone of a characteristic "late" gene, 16C8.…”

Section: Introductionmentioning

confidence: 99%

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A growth-responsive gene (16C8) in normal mouse fibroblasts homologous to a human collagenase inhibitor with erythroid-potentiating activity: evidence for inducible and constitutive transcripts

et al. 1986

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We present the DNA sequence of an essentially full-length cDNA clone of 16C8, a growth factor-inducible gene isolated from a mouse embryo fibroblast cDNA library. The 0.9-kb mRNA encodes an Mr 22,500 protein that has substantial homology to a human protein with the reported abilities (1) to potentiate erythroid differentiation and (2) to inhibit collagenases and other tissue metalloproteinases. The N-terminus of the predicted protein has a hydrophobic nature characteristic of secreted proteins, and two potential sites for Nlinked glycosylation are present. The cytoplasmic concentration of 16C8 mRNA is maximal in mid Gl at about 6 h after serum stimulation of quiescent fibroblasts. Northern blot analysis showed a progressive reduction in the size of the induced 16C8 transcripts with increasing time after serum stimulation. This was shown to be due to the reduction in length of the poly(A) tails. S1 analysis of the 5' portion of the mRNA revealed the presence of three different species of transcript, only one of which was inducible.

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Transcriptional regulation of two serum-induced RNAs in mouse fibroblasts: equivalence of one species to B2 repetitive elements.

Cited by 126 publications

References 40 publications

Expression of transforming growth factors beta-1, beta 2 and beta 3 in human bladder carcinomas

Expression of transforming growth factors beta-1, beta 2 and beta 3 in human bladder carcinomas

Isolation and characterization of a variant myoblast cell line that is temperature sensitive for differentiation.

A growth-responsive gene (16C8) in normal mouse fibroblasts homologous to a human collagenase inhibitor with erythroid-potentiating activity: evidence for inducible and constitutive transcripts

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