Transcriptional regulation of the carbon monoxide dehydrogenase gene (cdhA) in Methanosarcina thermophila.

Sowers, Kevin R.; Thai, Tung T.; Gunsalus, Robert P.

doi:10.1016/s0021-9258(19)49443-x

Cited by 37 publications

(7 citation statements)

References 44 publications

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“…An operon has been described in M. thermophila encoding the five subunits of the synthase for which up-regulation in response to growth on acetate has been described. 6 As noted in Part I, 12 the arrangement of genes in the M. thermophila operon are identical with that of both gene clusters (MA1011-1016 and MA3860-3865) predicted to encode the synthase paralogs in M. acetivorans. This gene arrangement in M. acetivorans is consistent with up-regulation of duplicate operons in response to the growth substrate, supporting the suggestion in Part I that both synthase paralogs in M. acetivorans serve an important function during growth on acetate.…”

Section: Resultsmentioning

confidence: 63%

“…Although it was not possible to determine quantitatively the relative contributions of the overlapping subunits, the results indicate that one or both of the subunits in each spot are elevated in acetate- versus methanol-grown cells. An operon has been described in M. thermophila encoding the five subunits of the synthase for which up-regulation in response to growth on acetate has been described . As noted in Part I, the arrangement of genes in the M. thermophila operon are identical with that of both gene clusters (MA1011−1016 and MA3860−3865) predicted to encode the synthase paralogs in M. acetivorans .…”

Section: Resultsmentioning

confidence: 92%

“…For example, it is reported that Methanosarcina species, when cultured with acetate, down-regulate the synthesis of proteins specific for the conversion of methanol to methane, and when cultured with methanol, down-regulate the synthesis of proteins specific for the metabolism of acetate. [6][7][8][9] This property can be exploited with proteomic approaches to identify novel proteins essential for specific cellular responses to growth conditions. This global experimental approach is expected to provide a foundation for understanding the systems biology of the M. acetivorans cell.…”

Section: Introductionmentioning

confidence: 99%

“…For example, Methanosarcina species are capable of growth and methanogenesis with acetate and several single-carbon compounds such as methanol. − Not surprisingly, these microbes have evolved mechanisms for regulation of protein synthesis in response to growth conditions. For example, it is reported that Methanosarcina species, when cultured with acetate, down-regulate the synthesis of proteins specific for the conversion of methanol to methane, and when cultured with methanol, down-regulate the synthesis of proteins specific for the metabolism of acetate. − This property can be exploited with proteomic approaches to identify novel proteins essential for specific cellular responses to growth conditions. This global experimental approach is expected to provide a foundation for understanding the systems biology of the M. acetivorans cell.…”

Section: Introductionmentioning

confidence: 99%

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Proteome of Methanosarcina acetivorans Part II: Comparison of Protein Levels in Acetate- and Methanol-Grown Cells

Rejtar

et al. 2005

J. Proteome Res.

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Methanosarcina acetivorans is an archaeon isolated from marine sediments which utilizes a diversity of substrates for growth and methanogenesis. Part I of a two-part investigation has profiled proteins of this microorganism cultured with both methanol and acetate as growth substrates, utilizing two-dimensional gel electrophoresis and MALDI-TOF-TOF mass spectrometry. In this report, Part II, the analyses were extended to identify 34 proteins found to be present in different amounts between methanol- and acetate-grown M. acetivorans. Among these proteins are enzymes which function in pathways for methanogenesis from either acetate or methanol. Several of the 34 proteins were determined to have redundant functions based on annotations of the genomic sequence. Enzymes which function in ATP synthesis and steps common to both methanogenic pathways were elevated in acetate- versus methanol-grown cells, whereas enzymes that have a more general function in protein synthesis were in greater amounts in methanol- compared to acetate-grown cells. Several group I chaperonins were present in greater amounts in methanol- versus acetate-grown cells, whereas lower amounts of several stress related proteins were found in methanol- versus acetate-grown cells. The potential physiological basis for these novel patterns of protein synthesis are discussed.

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Section: Resultsmentioning

confidence: 63%

Section: Resultsmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Proteome of Methanosarcina acetivorans Part II: Comparison of Protein Levels in Acetate- and Methanol-Grown Cells

Rejtar

et al. 2005

J. Proteome Res.

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“…Relatively little is known concerning transcriptional regulation in the methanogenic Archaea. Methanosarcina species are the most metabolically diverse among the Archaea, and show extensive regulation in response to changes in growth conditions 29,[59][60][61][62][63][64] which affords opportunities for understanding principles of gene regulation in Methanosarcina and the Archaea domain. The basal transcription apparatus in the Archaea resembles that of the Eucarya domain involving a TATA-box binding protein (TBP) and a transcription factor B which complexes with a eucaryal-like RNA polymerase.…”

Section: Conservation Of Energymentioning

confidence: 99%

Proteome ofMethanosarcinaacetivoransPart I: An Expanded View of the Biology of the Cell

Rejtar

et al. 2005

J. Proteome Res.

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Methanosarcina acetivorans is representative of the genus that is distinguished from all other methane-producing genera by extensive metabolic diversity predicted from the large genome. In Part I of this study, two-dimensional gel electrophoresis and MALDI-TOF-TOF mass spectrometry was used to investigate the proteome of methanol- or acetate-grown M. acetivorans, with the goal of an initial characterization of the diversity of the proteins synthesized. A total of 412 proteins were identified, representing nearly 10% of the ORFs, with nearly 30% conserved hypothetical or hypothetical. Of the 412 proteins, 188 were found in both acetate- and methanol-grown cells, 122 were detected only in acetate-grown cells, and 102 only in methanol-grown cells. The results revealed the expression of a remarkable number of redundant genes which encode enzymes involved in the pathways for methanogenesis from methanol or acetate, suggesting an important role for the unusually high percentage of redundant genes in Methanosarcina species. Evidence was obtained for synthesis of a sodium-transporting oxidoreductase in acetate-grown cells, with the potential to function in energy conservation. Several transcriptional regulatory proteins were identified that also function in the Bacteria domain, raising questions regarding their interaction with the Archaea/Eucarya-type basal transcription apparatus. In addition, a significant number of proteins involved in protein folding were shown to be synthesized in methanol- and acetate-grown cells. These studies provide the first examination of the protein diversity of M. acetivorans.

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Enzymology of the fermentation of acetate to methane by Methanosarcina thermophila

Ferry

1997

BioFactors

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Biologically-produced CH4 derives from either the reduction of CO2 or the methyl group of acetate by two separate pathways present in anaerobic mierobes from the Archaea domain. Elucidation of the pathway for CO2 reduction to CH4, the first to be investigated, has yielded several novel enzymes and cofactors. Most of the CH4 produced in nature derives from the methyl group of acetate. Methanosarcina thermophila is a moderate thermophile which ferments acetate by reducing the methyl group to CH4 with electrons derived from oxidation of the carbonyl group to CO2. The pathway in M. thermophila is now understood on a biochemical and genetic level comparable to understanding of the CO2-reducing pathway. Enzymes have been purified and characterized. The genes encoding these enzymes have been cloned, sequenced, transcriptionally mapped, and their regulation defined on a molecular level. This review emphasizes recent developments concerning the enzymes which are unique to the acetate fermentation pathway in M. thermophila.

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Transcriptional regulation of the carbon monoxide dehydrogenase gene (cdhA) in Methanosarcina thermophila.

Cited by 37 publications

References 44 publications

Proteome of Methanosarcina acetivorans Part II: Comparison of Protein Levels in Acetate- and Methanol-Grown Cells

Proteome of Methanosarcina acetivorans Part II: Comparison of Protein Levels in Acetate- and Methanol-Grown Cells

Proteome ofMethanosarcinaacetivoransPart I: An Expanded View of the Biology of the Cell

Enzymology of the fermentation of acetate to methane by Methanosarcina thermophila

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