“…Whole cell lysates from transfected/nontransfected cells were prepared after the indicated treatments as described previously. 29,30 The primary antibodies were as follows: LAMP2A (Abcam, ab18528, 1 μg/ml); LAMP2B, (Abcam, ab18529 1 μg/ml); LAMP1 (Cell Signaling, C54H11, 1:1000); HSPA8 (Santa Cruz Biotechnology, sc1059, 1:200); CASP3 (Imgenex, IMG-144A, 5 μg/ml); BAX (Santa Cruz Biotechnology, sc526, 1:200); BCL2 (Santa Cruz Biotechnology, sc-7382, 1:200); GAPDH (Imgenex, IMG5143A, 1 μg/ml); PKM (Cell Signaling, 3186, 1:1000); AKT1 (Cell Signaling, 9272, 1:1000); phospho-AKT1 (ser473) (Cell Signaling, 4051S, 1:1000); TUBB (Imgenex, IMG-5810A, 1 μg/ml) and ACTB (Imgenex, IMG-5142A, 1 μg/ml).…”