Transcriptional regulation of styrene degradation in Pseudomonas putida CA-3 The GenBank accession number for the sequence determined in this work is AF257095.

Abstract

The styrene degradative pathway in Pseudmonas putida CA-3 has previously been shown to be divided into an upper pathway involving the conversion of styrene to phenylacetic acid and a lower pathway for the subsequent degradation of phenylacetic acid. It is reported here that expression of the regulatory genes styS and styR is essential for transcription of the upper pathway, but not for degradation of the lower pathway inducer, phenylacetic acid. The presence of phenylacetic acid in the growth medium completely repressed the upper pathway enzymes even in the presence of styrene, the upper pathway inducer. This repression is mediated at the transcription level by preventing expression of the styS and styR regulatory genes. Finally, an examination was made of the various stages of the diauxic growth curve obtained when P. putida CA-3 was grown on styrene together with an additional carbon source and it is reported that catabolite repression may involve a different mechanism to transcriptional repression by an additional carbon source.

Expansion of growth substrate range in Pseudomonas putida F1 by mutations in both cymR and todS, which recruit a ring-fission hydrolase CmtE and induce the tod catabolic operon, respectively

“…In this regard, both TodS and TodT proteins are known to be required for the chemotaxis to aromatic hydrocarbons in P. putida F1 (Parales et al, 2000), indicating that the catabolism and microbial behaviour are co-ordinately regulated, and the chemotaxis is probably controlled at the transcriptional level. Similar two-component signal transduction systems have been identified in the pathways of aerobic styrene degradation (O'Leary et al, 2002;Panke et al, 1999;Santos et al, 2000;Velasco et al, 1998), in other forms of aerobic toluene degradation (Mosqueda et al, 1999), and in anaerobic toluene degradation (Coschigano & Young, 1997;Leuthner & Heider, 1998). The sensor proteins found in anaerobic toluene degradation are shorter than TodS and contain two PAS domains and one histidine kinase domain.…”

Analysis of the Pseudomonas putida CA-3 proteome during growth on styrene under nitrogen-limiting and non-limiting conditions

“…The genes encoding the upper pathway are organized on the styABCD operon, which is regulated at the transcriptional level by the StySR two-component sensory apparatus in this strain (O'Leary et al, 2001) and other species such as Pseudomonas fluorescens ST (Beltrametti et al, 1997) and Pseudomonas sp. Y2 (Bartolomé-Martin et al, 2004;Velasco et al, 1998).…”

Styrene oxide isomerase of Sphingopyxis sp. Kp5.2

“…Genes encoding the relevant enzymes for the upper pathway are usually clustered and ordered as sty(SR)ABCD (Beltrametti et al, 1997;O'Leary et al, 2001;Panke et al, 1998;Toda & Itoh, 2012;Velasco et al, 1998). The genes styS and styR encode a two-component regulatory element of the sty operon (O'Leary et al, 2001), and have so far been found only in pseudomonads (O'Leary et al, 2001;Panke et al, 1998;Velasco et al, 1998).…”

“…The genes styS and styR encode a two-component regulatory element of the sty operon (O'Leary et al, 2001), and have so far been found only in pseudomonads (O'Leary et al, 2001;Panke et al, 1998;Velasco et al, 1998).…”