Transcriptional regulation of Rab32/38, a specific marker of pigment cell formation in Ciona robusta

Racioppi, Claudia; Coppola, Ugo; Christiaen, Lionel; Ristoratore, Filomena

doi:10.1016/j.ydbio.2018.11.013

Cited by 8 publications

(8 citation statements)

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“…Changes in chromatin accessibility did not correlate precisely with changes in gene expression and several peaks near differentially expressed genes were not differentially accessible. Such decoupling between enhancer accessibility and activity has been observed in other developmental contexts, including early cardiogenesis (Racioppi et al, 2019;Paige et al, 2012;Wamstad et al, 2012). However, we found 53 (Tomato+ population) and 65 (GFP+ population) peaks correlating with changes in gene expression ( Figure 3E).…”

Section: Transcriptomic and Epigenetic Profiling Of The Shfsupporting

confidence: 76%

Hox-dependent coordination of mouse cardiac progenitor cell patterning and differentiation

Stefanovic

Laforest

Desvignes

et al. 2020

eLife

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Perturbation of addition of second heart field (SHF) cardiac progenitor cells to the poles of the heart tube results in congenital heart defects (CHD). The transcriptional programs and upstream regulatory events operating in different subpopulations of the SHF remain unclear. Here, we profile the transcriptome and chromatin accessibility of anterior and posterior SHF sub-populations at genome-wide levels and demonstrate that Hoxb1 negatively regulates differentiation in the posterior SHF. Spatial mis-expression of Hoxb1 in the anterior SHF results in hypoplastic right ventricle. Activation of Hoxb1 in embryonic stem cells arrests cardiac differentiation, whereas Hoxb1-deficient mouse embryos display premature cardiac differentiation. Moreover, ectopic differentiation in the posterior SHF of embryos lacking both Hoxb1 and its paralog Hoxa1 results in atrioventricular septal defects. Our results show that Hoxb1 plays a key role in patterning cardiac progenitor cells that contribute to both cardiac poles and provide new insights into the pathogenesis of CHD.

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Section: Transcriptomic and Epigenetic Profiling Of The Shfsupporting

confidence: 76%

Hox-dependent coordination of mouse cardiac progenitor cell patterning and differentiation

Stefanovic

Laforest

Desvignes

et al. 2020

eLife

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“…Interestingly, Mitf shows sustained expression in the otolith at later developmental stages (Figure 4; Abitua et al, 2012) confirming that this factor could be the final node of the regulatory network underlying the fate determination between otolith and ocellus pigment cells in Ciona as already suggested by Abitua et al (2012). Besides, Mitf controls also the expression of Rab32/38, a conserved melanogenic marker (Racioppi et al, 2019). The role of Mitf in pigment cell development has been observed also in Drosophila melanogaster (Hallsson et al, 2004), and the function of Mitf as master regulator of genes related to pigment cells and pigmentation is conserved in vertebrates (Levy et al, 2006;Vachtenheim and Borovanský, 2010).…”

Section: Discussionsupporting

confidence: 65%

“…Our findings represent the first expression data for Klhl21/30 subfamily in the chordate nervous system. In C. robusta, Klhl21/30 is initially transcribed in two cells of the pigment cell lineage alongside well-known markers of pigmented cells such as Tyr, Tyrp, and Rab32/38 (Coppola et al, 2016;Racioppi et al, 2019), which are conserved in chordate pigment cell evolution (Esposito et al, 2012;Coppola et al, 2016). Interestingly, Klhl21/30 became restricted to the otolith precursor from middle tailbud stage onward.…”

Section: Discussionmentioning

confidence: 99%

The Cis-Regulatory Code for Kelch-like 21/30 Specific Expression in Ciona robusta Sensory Organs

Coppola

Kamal

Stolfi

et al. 2020

Front. Cell Dev. Biol.

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The tunicate Ciona robusta is an emerging model system to study the evolution of the nervous system. Due to their small embryos and compact genomes, tunicates, like Ciona robusta, have great potential to comprehend genetic circuitry underlying cell specific gene repertoire, among different neuronal cells. Their simple larvae possess a sensory vesicle comprising two pigmented sensory organs, the ocellus and the otolith. We focused here on Klhl21/30, a gene belonging to Kelch family, that, in Ciona robusta, starts to be expressed in pigmented cell precursors, becoming specifically maintained in the otolith precursor during embryogenesis. Evolutionary analyses demonstrated the conservation of Klhl21/30 in all the chordates. Cis-regulatory analyses and CRISPR/Cas9 mutagenesis of potential upstream factors, revealed that Klhl21/30 expression is controlled by the combined action of three transcription factors, Mitf, Dmrt, and Msx, which are downstream of FGF signaling. The central role of Mitf is consistent with its function as a fundamental regulator of vertebrate pigment cell development. Moreover, our results unraveled a new function for Dmrt and Msx as transcriptional co-activators in the context of the Ciona otolith.

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“…Our group demonstrated that FGF signaling induces pigment cell identity in the blastomeres that, in absence of this signal, would adopt an anterior neural fate ( Racioppi et al, 2014 ). Among the genes identified in this analysis, Rab32/38 , a small GTPase involved in molecular trafficking, has been studied and demonstrated, by mutational analyses, to play a role in the pigmentation of Ciona larval sensory organs ( Racioppi et al, 2014 , 2019b ).…”

Section: Gene Regulatory Network Underlying Pigmented Cells Specificationmentioning

confidence: 99%

Brain Sensory Organs of the Ascidian Ciona robusta: Structure, Function and Developmental Mechanisms

Olivo

Palladino

Ristoratore

2021

Front. Cell Dev. Biol.

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During evolution, new characters are designed by modifying pre-existing structures already present in ancient organisms. In this perspective, the Central Nervous System (CNS) of ascidian larva offers a good opportunity to analyze a complex phenomenon with a simplified approach. As sister group of vertebrates, ascidian tadpole larva exhibits a dorsal CNS, made up of only about 330 cells distributed into the anterior sensory brain vesicle (BV), connected to the motor ganglion (MG) and a caudal nerve cord (CNC) in the tail. Low number of cells does not mean, however, low complexity. The larval brain contains 177 neurons, for which a documented synaptic connectome is now available, and two pigmented organs, the otolith and the ocellus, controlling larval swimming behavior. The otolith is involved in gravity perception and the ocellus in light perception. Here, we specifically review the studies focused on the development of the building blocks of ascidians pigmented sensory organs, namely pigment cells and photoreceptor cells. We focus on what it is known, up to now, on the molecular bases of specification and differentiation of both lineages, on the function of these organs after larval hatching during pre-settlement period, and on the most cutting-edge technologies, like single cell RNAseq and genome editing CRISPR/CAS9, that, adapted and applied to Ciona embryos, are increasingly enhancing the tractability of Ciona for developmental studies, including pigmented organs formation.

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Transcriptional regulation of Rab32/38, a specific marker of pigment cell formation in Ciona robusta

Cited by 8 publications

References 50 publications

Hox-dependent coordination of mouse cardiac progenitor cell patterning and differentiation

Hox-dependent coordination of mouse cardiac progenitor cell patterning and differentiation

The Cis-Regulatory Code for Kelch-like 21/30 Specific Expression in Ciona robusta Sensory Organs

Brain Sensory Organs of the Ascidian Ciona robusta: Structure, Function and Developmental Mechanisms

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