Transcriptional regulation of peripheral glial cell differentiation in the embryonic nervous system of drosophila

Schmidt, Imke; Franzdóttir, Sigríður Rut; Edenfeld, Gundula; Rodrigues, Floriano; Zierau, Ariane; Klämbt, Christian

doi:10.1002/glia.21123

Cited by 8 publications

(5 citation statements)

References 102 publications

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“…Another putative target gene of Hb is Ets98b ( Fig 8B ), the ortholog of which has recently been shown to induce ectopic cell migration upon misexpression during early embryonic development in the common house spider Parasteatoda tepidariorum [ 152 ]. Finally, Nejire has been shown to be involved in glia cell migration in the peripheral nervous system in Drosophila [ 153 ], and we found it as putative regulator of genes in cluster 13. It remains to be established, whether Nejire and Hb may collaborate during carpet cell development.…”

Section: Discussionmentioning

confidence: 66%

Dynamic genome wide expression profiling of Drosophila head development reveals a novel role of Hunchback in retinal glia cell development and blood-brain barrier integrity

Torres-Oliva¹,

Schneider²,

Wiegleb³

et al. 2018

PLoS Genet

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Drosophila melanogaster head development represents a valuable process to study the developmental control of various organs, such as the antennae, the dorsal ocelli and the compound eyes from a common precursor, the eye-antennal imaginal disc. While the gene regulatory network underlying compound eye development has been extensively studied, the key transcription factors regulating the formation of other head structures from the same imaginal disc are largely unknown. We obtained the developmental transcriptome of the eye-antennal discs covering late patterning processes at the late 2nd larval instar stage to the onset and progression of differentiation at the end of larval development. We revealed the expression profiles of all genes expressed during eye-antennal disc development and we determined temporally co-expressed genes by hierarchical clustering. Since co-expressed genes may be regulated by common transcriptional regulators, we combined our transcriptome dataset with publicly available ChIP-seq data to identify central transcription factors that co-regulate genes during head development. Besides the identification of already known and well-described transcription factors, we show that the transcription factor Hunchback (Hb) regulates a significant number of genes that are expressed during late differentiation stages. We confirm that hb is expressed in two polyploid subperineurial glia cells (carpet cells) and a thorough functional analysis shows that loss of Hb function results in a loss of carpet cells in the eye-antennal disc. Additionally, we provide for the first time functional data indicating that carpet cells are an integral part of the blood-brain barrier. Eventually, we combined our expression data with a de novo Hb motif search to reveal stage specific putative target genes of which we find a significant number indeed expressed in carpet cells.

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Section: Discussionmentioning

confidence: 66%

Dynamic genome wide expression profiling of Drosophila head development reveals a novel role of Hunchback in retinal glia cell development and blood-brain barrier integrity

Torres-Oliva¹,

Schneider²,

Wiegleb³

et al. 2018

PLoS Genet

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“…A mutant strain of sim (sim 2/H9 kar 1 /TM3, P[ftz-lacZ] SC1, Sb 1 ry RK ; stock number 2055) was obtained from the Bloomington Stock Center. The sim 2/H9 allele is an amorphic allele, and mutant embryos homozygous for the allele are characterized by the absence of all midline cells after migration of only a few peripheral glial cells (Schmidt et al 2011). Mutant embryos homozygous for sim 2/H9 were distinguished from heterozygotes by the lack of lacZ expression in fushi tarazu (ftz) expression domains mediated by the ftz-lacZ fusion gene of the TM3 balancer chromosome.…”

Section: Fly Stockmentioning

confidence: 99%

The shadow enhancer of short gastrulation also directs its expression in the ventral midline of the Drosophila embryo

Shin

Hong

2015

Genes Genom

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The sog, a fly homologue of the vertebrate chordin, is expressed in the presumptive neurogenic ectoderm and also the ventral midline during Drosophila embryogenesis. Previously, two enhancers, called the primary and shadow enhancer, respectively, were found to direct sog expression in the neurogenic ectoderm. However, efforts to identify an enhancer that directs sog expression in the ventral midline have failed. Here, we present evidence that the sog shadow enhancer has dual activities to direct sog expression in the neurogenic ectoderm as well as the ventral midline. Systematic truncation analyses employing transgenic embryos revealed that about a 680-bp region within the shadow enhancer is necessary and sufficient for midline enhancer activity. Intriguingly, midline enhancer activity does not appear to depend on binding sites for Sim, because distal and proximal elements critical for sog midline expression within the 680-bp region do not have any canonical Sim binding sites.

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“…The transcription factor Pointed promotes glial differentiation and the Zn-finger protein Tramtrack inhibits neuronal differentiation in glial cells (Klaes et al, 1994 ; Giesen et al, 1997 ). Together with other transcriptional regulators such as Prospero, Distal-less and Deadringer, these factors most likely are involved in specifying the glial subtype identity (Shandala et al, 2003 ; Thomas and van Meyel, 2007 ; Schmidt et al, 2011 ). The relevant transcriptional regulators that specify blood-brain barrier identity are currently unknown.…”

Section: Drosophila Glial Cellsmentioning

confidence: 99%

The Drosophila blood-brain barrier: development and function of a glial endothelium

et al. 2014

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The efficacy of neuronal function requires a well-balanced extracellular ion homeostasis and a steady supply with nutrients and metabolites. Therefore, all organisms equipped with a complex nervous system developed a so-called blood-brain barrier, protecting it from an uncontrolled entry of solutes, metabolites or pathogens. In higher vertebrates, this diffusion barrier is established by polarized endothelial cells that form extensive tight junctions, whereas in lower vertebrates and invertebrates the blood-brain barrier is exclusively formed by glial cells. Here, we review the development and function of the glial blood-brain barrier of Drosophila melanogaster. In the Drosophila nervous system, at least seven morphologically distinct glial cell classes can be distinguished. Two of these glial classes form the blood-brain barrier. Perineurial glial cells participate in nutrient uptake and establish a first diffusion barrier. The subperineurial glial (SPG) cells form septate junctions, which block paracellular diffusion and thus seal the nervous system from the hemolymph. We summarize the molecular basis of septate junction formation and address the different transport systems expressed by the blood-brain barrier forming glial cells.

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Transcriptional regulation of peripheral glial cell differentiation in the embryonic nervous system of drosophila

Cited by 8 publications

References 102 publications

Dynamic genome wide expression profiling of Drosophila head development reveals a novel role of Hunchback in retinal glia cell development and blood-brain barrier integrity

Dynamic genome wide expression profiling of Drosophila head development reveals a novel role of Hunchback in retinal glia cell development and blood-brain barrier integrity

The shadow enhancer of short gastrulation also directs its expression in the ventral midline of the Drosophila embryo

The Drosophila blood-brain barrier: development and function of a glial endothelium

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