Transcriptional regulation of miR-30a by YAP impacts PTPN13 and KLF9 levels and Schwann cell proliferation

Abstract

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MicroRNA-30a inhibits cell proliferation in a sepsis-induced acute kidney injury model by targeting the YAP-TEAD complex

“…While the function of the YAP-TEAD complex has been extensively characterized, only a few studies have examined YAP function as a regulator of gene expression via microRNAs. [ 34 , 35 ] A recent study revealed that YAP binds to the promoter and regulates the expression of miR-30a. YAP regulation of Schwann cell proliferation and death is mediated through miR-30a regulation of protein tyrosine phosphatase non-receptor type 13 (PTPN13) in Schwann cells.…”

“…YAP regulation of Schwann cell proliferation and death is mediated through miR-30a regulation of protein tyrosine phosphatase non-receptor type 13 (PTPN13) in Schwann cells. [ 34 ] Hence, there are complex mutual regulation relationships between miR-30a and the YAP-TEAD complex, which require further study and clarification. These regulatory mechanisms are not limited to specific disease environments, such as sepsis-AKI, nor do they only occur in Schwann cells.…”

Hippo Pathway in Schwann Cells and Regeneration of Peripheral Nervous System

“…In immature SCs, YAP/TAZ are found at high nuclear levels and promote vigorous proliferation [17, 31, 32]. In actively proliferating human Schwann (hSC2λ) cell model, YAP could promote proliferation through inducing the expression of miR-30a, which is a microRNA that could downregulate the growth-suppressive protein PTPN13 [18]. In YapTaz dKO immature SCs, genes related to cell growth such as Amotl2, Fgf1, Ect2 , and Ddah1 were significantly downregulated; a dramatic drop was also observed in the expression of cell cycle-associated genes Ccnj , Cdk6 , Pim3 , and Mycn [17].…”

“…Hippo pathway shows the magic of switching between promoting proliferation and facilitating myelination in neural crest cell (NCC) and SC development [15][16][17][18]. The brief introduction of the development of SCs and the role of Hippo pathway during the development will be elaborated on below.…”

Emerging Role of MicroRNA-30c in Neurological Disorders

“…The cell uses a variety of tactics such as RNA-binding proteins, transcription factors, and protein and RNA enzymes (e.g., exoribonucleases and endoribonucleases) to inhibit or promote each phase [5]. A recent study indicated that YAP (yes-associated protein, Hippo pathway) regulates miR-30a via TEA (transcriptional enhanced associate) domain (TEAD) transcription factors [24]. The methylation of miR-30 genes also causes their downregulation [25].…”