Transcriptional regulation of microalgae for concurrent lipid overproduction and secretion

Li, Da-Wei; Balamurugan, S.; Yang, Yufeng; Zheng, Jian; Huang, Dan; Zou, Li-Gong; Yang, Wei‐Dong; Liu, Jie‐Sheng; Guan, Yuanfang; Li, Hongye

doi:10.1126/sciadv.aau3795

Cited by 83 publications

(67 citation statements)

References 41 publications

(73 reference statements)

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“…Recently there have been several reports on microalgal lipid overproduction via metabolic engineering, particularly by overexpressing the rate‐limiting enzymes and transcription factors involved in lipogenesis and fatty acid biosynthesis (Balamurugan et al, ; Li et al, ; Niu et al, ; X. Wang et al, ). Precise regulation of gene expression has been considered a crucial factor in metabolic engineering (Topp & Gallivan, ).…”

Section: Discussionmentioning

confidence: 99%

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Potentiation of concurrent expression of lipogenic genes by novel strong promoters in the oleaginous microalga Phaeodactylum tricornutum

Zou

Balamurugan

Zhou

et al. 2019

Biotech & Bioengineering

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There has been growing interest in using microalgae as production hosts for a wide range of value‐added compounds. However, microalgal genetic improvement is impeded by lack of genetic tools to concurrently control multiple genes. Here, we identified two novel strong promoters, designated Pt202 and Pt667, and delineated their potential role on simultaneously driving the expression of key lipogenic genes in Phaeodactylum tricornutum. In silico analyses of the identified promoter sequences predicted the presence of essential core cis elements such as TATA and CAAT boxes. Regulatory role of the promoters was preliminarily assessed by using GUS reporter which demonstrated strong GUS expression. Thereafter, two key lipogenic genes including malic enzyme (PtME) and 5‐desaturase (PtD5b), were overexpressed by the two promoters Pt202 and Pt667, respectively, in P. tricornutum. Combinatorial gene overexpression did not impair general physiological performance, meanwhile neutral lipid content was remarkably increased by 2.4‐fold. GC‐MS analysis of fatty acid methyl esters revealed that eicosapentaenoic acid (EPA; C20:5) was increased significantly. The findings augment a crucial kit to microalgal genetic tools that could facilitate the multiple‐gene expression driven by various promoters, and promote microalgae for industrial bioproduction.

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Section: Discussionmentioning

confidence: 99%

“…Recently there have been several reports on microalgal lipid overproduction via metabolic engineering, particularly by overexpressing the rate-limiting enzymes and transcription factors involved in lipogenesis and fatty acid biosynthesis Li et al, 2019;Niu et al, 2016;X. Wang et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Potentiation of concurrent expression of lipogenic genes by novel strong promoters in the oleaginous microalga Phaeodactylum tricornutum

Zou

Balamurugan

Zhou

et al. 2019

Biotech & Bioengineering

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“…The up-regulation of a Dof-type transcription factor in Chlamydomonas doubled the cellular content of TAG [146,147]. Over-expression of bZIP and bHLH transcription factors in both N. salina and N. oceanica improved biomass and lipid productivity [148][149][150][151]. In N. gaditana, 20 putative regulators of lipid production, down-regulated by nitrogen deprivation, were identified by RNA-seq; a strategy of selective knock-out by the Crispr-Cas9 system allowed us to identify a homolog of the fungal Zn(II) 2 Cys 6 -encoding gene, which triggered lipid biosynthesis in Nannochloropsis and resulted in a 200% increase of C partition to lipids, without significantly affecting the growth rate [152].…”

Section: Engineering Of the Lipid Biosynthesis For Renewable Energiesmentioning

confidence: 99%

Potential and Challenges of Improving Photosynthesis in Algae

Vecchi

Barera

Bassi

et al. 2020

Plants

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Sunlight energy largely exceeds the energy required by anthropic activities, and therefore its exploitation represents a major target in the field of renewable energies. The interest in the mass cultivation of green microalgae has grown in the last decades, as algal biomass could be employed to cover a significant portion of global energy demand. Advantages of microalgal vs. plant biomass production include higher light-use efficiency, efficient carbon capture and the valorization of marginal lands and wastewaters. Realization of this potential requires a decrease of the current production costs, which can be obtained by increasing the productivity of the most common industrial strains, by the identification of factors limiting biomass yield, and by removing bottlenecks, namely through domestication strategies aimed to fill the gap between the theoretical and real productivity of algal cultures. In particular, the light-to-biomass conversion efficiency represents one of the major constraints for achieving a significant improvement of algal cell lines. This review outlines the molecular events of photosynthesis, which regulate the conversion of light into biomass, and discusses how these can be targeted to enhance productivity through mutagenesis, strain selection or genetic engineering. This review highlights the most recent results in the manipulation of the fundamental mechanisms of algal photosynthesis, which revealed that a significant yield enhancement is feasible. Moreover, metabolic engineering of microalgae, focused upon the development of renewable fuel biorefineries, has also drawn attention and resulted in efforts for enhancing productivity of oil or isoprenoids.

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“…Concurrent enhancement of biomass and desired products is considered a critical parameter for commercial applications (Li et al, 2019). Hence, we examined the impact of NoD6 overexpression on cellular physiological parameters.…”

Section: Nod6 Overexpression Enhanced Cellular Growth and Photosynthementioning

confidence: 99%

Harnessing the Lipogenic Potential of Δ6-Desaturase for Simultaneous Hyperaccumulation of Lipids and Polyunsaturated Fatty Acids in Nannochloropsis oceanica

Yang

Yuan

et al. 2019

Front. Mar. Sci.

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Burgeoning demand for long-chain polyunsaturated fatty acids (LC-PUFA) due to their established pharmacological and economic significances along with the declining sources of fish urge to explore the sustainable sources of LC-PUFA. Being the predominant LC-PUFA source for marine fishes at the base of the aquatic food web, microalgae has been hailed as a promising natural source for LC-PUFA. However, the potential of algal systems to overproduce LC-PUFA via metabolic engineering is warranted to meet the ever-increasing demand. In this study, we identified and overexpressed 6-desaturase, the key enzyme involved in fatty acid desaturation and exemplified its potential on elevating PUFA and lipid content in Nannochloropsis oceanica. 6-desaturase overexpression enhanced growth and photosynthetic efficiency. Transgenic cells exhibited a remarkable increase in EPA content and reached up to 62.35 mg/g DW, the highest EPA production in transgenic N. oceanica by expressing a single key enzyme without impeding growth. Total lipid content was significantly increased by 1.7-fold in the transgenic cell than WT. Together, these findings exemplify a potential candidate for LC-PUFA overproduction and also open a new avenue for sustainable production of microalgal PUFAs.

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Transcriptional regulation of microalgae for concurrent lipid overproduction and secretion

Abstract: NobZIP1 governs the expression of various key genes, leading to lipid overproduction and secretion in Nannochloropsis oceanica.

Cited by 83 publications

References 41 publications

Potentiation of concurrent expression of lipogenic genes by novel strong promoters in the oleaginous microalga Phaeodactylum tricornutum

Potentiation of concurrent expression of lipogenic genes by novel strong promoters in the oleaginous microalga Phaeodactylum tricornutum

Potential and Challenges of Improving Photosynthesis in Algae

Harnessing the Lipogenic Potential of Δ6-Desaturase for Simultaneous Hyperaccumulation of Lipids and Polyunsaturated Fatty Acids in Nannochloropsis oceanica

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