Transcriptional regulation of flavonoid biosynthesis in <i>Artemisia annua</i> by AaYABBY5

Kayani, Sadaf‐Ilyas; Shen, Qian; Rahman, Saeed-ur; Fu, Xueqing; Li, Yongpeng; Wang, Chen; Hassani, Danial; Tang, Kexuan

doi:10.1038/s41438-021-00693-x

Cited by 36 publications

(18 citation statements)

References 58 publications

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“…These cis ‐acting elements have been reported to be recognized by bZIP, bHLH, MYB, WRKY and AP2/ERF TFs to activate the phenylpropanoid pathway in different plants (Han et al., 2021; Hassani et al., 2020; Ji et al., 2021; Zhao et al., 2021; Zhu et al., 2020). For example, AaYABBY5 TF can directly bind to the promoter regions of AaPAL , AaCHI , AaCHS and AaUFGT to positively regulate anthocyanin and flavonoid biosynthesis in Artemisia annua (Kayani et al., 2021). Thus, there are potentially a number of other TFs that regulate these three genes ( MsMYB741 , MsPAL1 and MsCHI ) to synthesize flavonoid, but the ABA‐ABF2‐MYB741‐flavonoid module must be one of the important pathways in regulating flavonoid biosynthesis responsive to abiotic stress.…”

Section: Discussionmentioning

confidence: 99%

MsMYB741 is involved in alfalfa resistance to aluminum stress by regulating flavonoid biosynthesis

Wen

et al. 2022

The Plant Journal

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Aluminum (Al) toxicity severely restricts plant growth in acidic soils (pH < 5.0). In this study, an R2R3-MYB transcription factor (TF) gene, MsMYB741, was cloned from alfalfa. Its function and gene regulatory pathways were studied via overexpression and RNA interference of MsMYB741 in alfalfa seedlings. Results showed that root elongation increased as a result of MsMYB741 overexpression (MsMYB741-OE) and decreased with MsMYB741 RNA interference (MsMYB741-RNAi) in alfalfa seedlings compared with the wildtype under Al stress. These were attributed to the reduced Al content in MsMYB741-OE lines, and increased Al content in MsMYB741-RNAi lines. MsMYB741 positively activated the expression of phenylalanine ammonia-lyase 1 (MsPAL1) and chalcone isomerase (MsCHI) by binding to MYB and ABRE elements in their promoters, respectively, which directly affected flavonoid accumulation in roots and secretion from root tips in plants under Al stress, eventually affecting Al accumulation in alfalfa. Additionally, MsABF2 TF directly activated the expression of MsMYB741 by binding to the ABRE element in its promoter. Taken together, our results indicate that MsMYB741 transcriptionally activates MsPAL1 and MsCHI expression to increase flavonoid accumulation in roots and secretion from root tips, leading to increased resistance of alfalfa to Al stress.

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Section: Discussionmentioning

confidence: 99%

MsMYB741 is involved in alfalfa resistance to aluminum stress by regulating flavonoid biosynthesis

Wen

et al. 2022

The Plant Journal

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“…Furthermore, the abundance of TFs (viz., bHLH, NAC, ERF, WRKY, and MYB) indicates their role in mono- and sesquiterpenoid and phenylpropanoid biosynthesis, as reported in previous studies [ 53 , 54 ]. Likewise, the leaf-specific higher enrichments of WRKY, TCP, YABBY, ERF, and MYB suggest their key involvement in regulating terpenoid and flavonoid biosynthesis [ 28 , 55 , 56 , 57 ], while the root-specific enrichment of TFs (viz., Trihelix, WOX, and SBP) probably regulates secondary-metabolite biosynthesis [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

Spatial Genomic Resource Reveals Molecular Insights into Key Bioactive-Metabolite Biosynthesis in Endangered Angelica glauca Edgew

Devi

Seth

Masand

et al. 2022

IJMS

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Angelica glauca Edgew, which is an endangered medicinal and aromatic herb, is a rich source of numerous industrially important bioactive metabolites, including terpenoids, phenolics, and phthalides. Nevertheless, genomic interventions for the sustainable utilization and restoration of its genetic resources are greatly offset due to the scarcity of the genomic resources and key regulators of the underlying specialized metabolism. To unravel the global atlas of the specialized metabolism, the first spatial transcriptome sequencing of the leaf, stem, and root generated 109 million high-quality paired-end reads, assembled de novo into 81,162 unigenes, which exhibit a 61.53% significant homology with the six public protein databases. The organ-specific clustering grouped 1136 differentially expressed unigenes into four subclusters differentially enriched in the leaf, stem, and root tissues. The prediction of the transcriptional-interactome network by integrating enriched gene ontology (GO) and the KEGG metabolic pathways identified the key regulatory unigenes that correspond to terpenoid, flavonoid, and carotenoid biosynthesis in the leaf tissue, followed by the stem and root tissues. Furthermore, the stem and root-specific significant enrichments of phenylalanine ammonia lyase (PAL), cinnamate-4-hydroxylase (C4H), and caffeic acid 3-O-methyltransferase (COMT) indicate that phenylalanine mediated the ferulic acid biosynthesis in the stem and root. However, the root-specific expressions of NADPH-dependent alkenal/one oxidoreductase (NADPH-AOR), S-adenosyl-L-methionine-dependent methyltransferases (SDMs), polyketide cyclase (PKC), and CYP72A15 suggest the “root” as the primary site of phthalide biosynthesis. Additionally, the GC-MS and UPLC analyses corresponded to the organ-specific gene expressions, with higher contents of limonene and phthalide compounds in the roots, while there was a higher accumulation of ferulic acid in the stem, followed by in the root and leaf tissues. The first comprehensive genomic resource with an array of candidate genes of the key metabolic pathways can be potentially utilized for the targeted upscaling of aromatic and pharmaceutically important bioactive metabolites. This will also expedite genomic-assisted conservation and breeding strategies for the revival of the endangered A. glauca.

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“…In addition to the cuticle and trichome, the impact of flavonoid and terpenoid biosynthetic pathways, and trichome development was recently demonstrated in tomato and A. annua (Kang et al, 2014;Shi et al, 2018;Sugimoto et al, 2021). The af mutated tomato, AaYABBY5-overexpressed, and AaTAR2overexpressed A. annua all affect trichome density, flavonoid, and terpenoid biosynthesis (Kayani et al, 2019(Kayani et al, , 2021Zhou et al, 2020;Sugimoto et al, 2021). Our findings demonstrated that terpenoid-related genes and terpenoid metabolites were downregulated in plants with fewer PGTs, compared to controls; however, the variation in flavonoid contents in control plants and plants with fewer PGTs was not significant.…”

Section: Ntmixta Positively Influences Pgt Formation and Monoterpene ...mentioning

confidence: 98%

Identification and characterization of a novel gene involved in glandular trichome development in Nepeta tenuifolia

et al. 2022

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Nepeta tenuifolia is a medicinal plant rich in terpenoids and flavonoids with antiviral, immunoregulatory, and anti-inflammatory activities. The peltate glandular trichome (PGT) is a multicellular structure considered to be the primary storage organ for monoterpenes; it may serve as an ideal model for studying cell differentiation and the development of glandular trichomes (GTs). The genes that regulate the development of GTs have not yet been well studied. In this study, we identified NtMIXTA1, a GT development-associated gene from the R2R3 MYB SBG9 family. NtMIXTA1 overexpression in tobacco resulted in the production of longer and denser GTs. Virus-induced gene silencing of NtMIXTA1 resulted in lower PGT density, a significant reduction in monoterpene concentration, and the decreased expression of genes related to monoterpene biosynthesis. Comparative transcriptome and widely targeted metabolic analyses revealed that silencing NtMIXTA1 significantly influenced the expression of genes, and the production of metabolites involved in the biosynthesis of terpenoids, flavonoids, and lipids. This study provides a solid foundation describing a mechanism underlying the regulation of GT development. In addition, this study further deepens our understanding of the regulatory networks involved in GT development and GT development-associated metabolite flux, as well as provides valuable reference data for studying plants with a high medicinal value without genetic transformation.

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Transcriptional regulation of flavonoid biosynthesis in Artemisia annua by AaYABBY5

Cited by 36 publications

References 58 publications

MsMYB741 is involved in alfalfa resistance to aluminum stress by regulating flavonoid biosynthesis

MsMYB741 is involved in alfalfa resistance to aluminum stress by regulating flavonoid biosynthesis

Spatial Genomic Resource Reveals Molecular Insights into Key Bioactive-Metabolite Biosynthesis in Endangered Angelica glauca Edgew

Identification and characterization of a novel gene involved in glandular trichome development in Nepeta tenuifolia

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