Transcriptional regulation of drought response: a tortuous network of transcriptional factors

Singh, Dhriti; Laxmi, Ashverya

doi:10.3389/fpls.2015.00895

Cited by 337 publications

(294 citation statements)

References 95 publications

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“…MYB is the largest TF family regulated by drought stress in both cultivars followed by bHLH and NAC respectively in DT and DS. These TFs might play crucial roles in stress response and our results were consistent with previous studies which indicated that AP2/EREBP, bHLH, bZIP, HD-ZIP, HSF, MYB, NAC, WRKY, and zinc-finger protein TFs had vital roles in the plant response to drought stress (Davey et al, 2009; Mizoi et al, 2012; Sakuraba et al, 2015; Singh and Laxmi, 2015). The heat stress could induce expression of HSFs to protect plants from adverse effects of heat stress (Guo et al, 2016).…”

Section: Discussionsupporting

confidence: 92%

“…WRKY42 involved in negative regulation of transcription. Most of the other up-regulated TF families, such as WRKY, NAC, C2H2, and HSF, participate in drought stress response (Huang et al, 2008; Nishizawa et al, 2008; Sakuraba et al, 2015; Singh and Laxmi, 2015). Particularly, some classes of bHLH TFs were known to bind with CBF genes (DREB1 and 2) under stress conditions and their interaction will contribute to abiotic stress tolerance including drought tolerance (Sreenivasulu et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

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Transcriptomic Changes of Drought-Tolerant and Sensitive Banana Cultivars Exposed to Drought Stress

et al. 2016

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In banana, drought responsive gene expression profiles of drought-tolerant and sensitive genotypes remain largely unexplored. In this research, the transcriptome of drought-tolerant banana cultivar (Saba, ABB genome) and sensitive cultivar (Grand Naine, AAA genome) was monitored using mRNA-Seq under control and drought stress condition. A total of 162.36 million reads from tolerant and 126.58 million reads from sensitive libraries were produced and mapped onto the Musa acuminata genome sequence and assembled into 23,096 and 23,079 unigenes. Differential gene expression between two conditions (control and drought) showed that at least 2268 and 2963 statistically significant, functionally known, non-redundant differentially expressed genes (DEGs) from tolerant and sensitive libraries. Drought has up-regulated 991 and 1378 DEGs and down-regulated 1104 and 1585 DEGs respectively in tolerant and sensitive libraries. Among DEGs, 15.9% are coding for transcription factors (TFs) comprising 46 families and 9.5% of DEGs are constituted by protein kinases from 82 families. Most enriched DEGs are mainly involved in protein modifications, lipid metabolism, alkaloid biosynthesis, carbohydrate degradation, glycan metabolism, and biosynthesis of amino acid, cofactor, nucleotide-sugar, hormone, terpenoids and other secondary metabolites. Several, specific genotype-dependent gene expression pattern was observed for drought stress in both cultivars. A subset of 9 DEGs was confirmed using quantitative reverse transcription-PCR. These results will provide necessary information for developing drought-resilient banana plants.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Transcriptomic Changes of Drought-Tolerant and Sensitive Banana Cultivars Exposed to Drought Stress

et al. 2016

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“…In contrast to the CBPs, PKs are sensor responders (Batistič and Kudla, 2012) that initiate phosphorylation cascades and thereby play important roles in responses to drought stress (Singh and Laxmi, 2015). The genes of STKs and PKs were upregulated which indicates the initiation of phosphorylation cascades.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional Responses in Root and Leaf of Prunus persica under Drought Stress Using RNA Sequencing

et al. 2016

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Prunus persica L. Batsch, or peach, is one of the most important crops and it is widely established in irrigated arid and semi-arid regions. However, due to variations in the climate and the increased aridity, drought has become a major constraint, causing crop losses worldwide. The use of drought-tolerant rootstocks in modern fruit production appears to be a useful method of alleviating water deficit problems. However, the transcriptomic variation and the major molecular mechanisms that underlie the adaptation of drought-tolerant rootstocks to water shortage remain unclear. Hence, in this study, high-throughput sequencing (RNA-seq) was performed to assess the transcriptomic changes and the key genes involved in the response to drought in root tissues (GF677 rootstock) and leaf tissues (graft, var. Catherina) subjected to 16 days of drought stress. In total, 12 RNA libraries were constructed and sequenced. This generated a total of 315 M raw reads from both tissues, which allowed the assembly of 22,079 and 17,854 genes associated with the root and leaf tissues, respectively. Subsets of 500 differentially expressed genes (DEGs) in roots and 236 in leaves were identified and functionally annotated with 56 gene ontology (GO) terms and 99 metabolic pathways, which were mostly associated with aminobenzoate degradation and phenylpropanoid biosynthesis. The GO analysis highlighted the biological functions that were exclusive to the root tissue, such as “locomotion,” “hormone metabolic process,” and “detection of stimulus,” indicating the stress-buffering role of the GF677 rootstock. Furthermore, the complex regulatory network involved in the drought response was revealed, involving proteins that are associated with signaling transduction, transcription and hormone regulation, redox homeostasis, and frontline barriers. We identified two poorly characterized genes in P. persica: growth-regulating factor 5 (GRF5), which may be involved in cellular expansion, and AtHB12, which may be involved in root elongation. The reliability of the RNA-seq experiment was validated by analyzing the expression patterns of 34 DEGs potentially involved in drought tolerance using quantitative reverse transcription polymerase chain reaction. The transcriptomic resources generated in this study provide a broad characterization of the acclimation of P. persica to drought, shedding light on the major molecular responses to the most important environmental stressor.

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“…According to some reports, ABA promotes JA accumulation (Fan et al 2009). Furthermore, the presence of MYC and NAC transcription factors suggests the existence of cross-talk between JA and ABA signaling (Singh and Laxmi 2015). It should also be noted that ABA and JA antagonistically regulate the expression of salt stressinducible proteins (Moons et al 1997).…”

Section: Signal Transduction Transcription and Translation Processingmentioning

confidence: 99%

ABA pretreatment can limit salinity-induced proteome changes in growing barley sprouts

2017

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A proteomic study was conducted on caryopses of barley cv. Stratus (Hordeum vulgare). The caryopses were germinated in darkness at 20°C, according to one of the three experimental setups: (a) in distilled water for 24 h, followed by 100 mM NaCl for another 24 h (salinity stress, SS); (b) in 100 lM abscisic acid for 24 h, with rinsing in distilled water to remove residual ABA, followed by 100 mM NaCl for another 24 h (pretreatment with ABA ? salinity stress, ABAS); and (c) only in distilled water, for comparison with the tested samples (control, C). After 48 h, proteomes were extracted from barley sprouts in each treatment, according to the method by Gallardo et al. (2002a, b, modified). Proteins were then separated by 2DE and analyzed by MALDI-TOF/MS (Bruker). Under salinity stress, we observed changes in the pattern of proteins involved in: (a) energy metabolism, (b) one-carbon metabolism, (c) cell wall biogenesis, lignification, and adhesion, (d) response to oxidative stress, desiccation stress, and other defense responses, (e) structure and organization of the cytoskeleton, (f) signal transduction, transcription and translation processing, and (g) protein turnover. Our results revealed that ABA can alter the response of barley seedlings to salinity stress. In general, ABA pretreatment limited stress-induced proteomic changes. Alterations in energy and one-carbon metabolism indicate the importance of ABA in glycolytic/gluconeogenic pathways and the SMM cycle during salinity stress. Other observations demonstrated the role of ABA in hemicellulose, cellulose and lignin biosynthesis, ROS scavenging, actin CK formation, control of PI signaling, chromatin-mediated mechanism of stress tolerance, mRNA stability, and protection against dehydration.

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Transcriptional regulation of drought response: a tortuous network of transcriptional factors

Cited by 337 publications

References 95 publications

Transcriptomic Changes of Drought-Tolerant and Sensitive Banana Cultivars Exposed to Drought Stress

Transcriptomic Changes of Drought-Tolerant and Sensitive Banana Cultivars Exposed to Drought Stress

Transcriptional Responses in Root and Leaf of Prunus persica under Drought Stress Using RNA Sequencing

ABA pretreatment can limit salinity-induced proteome changes in growing barley sprouts

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