Transcriptional regulation of bone sialoprotein gene by interleukin-11

Wang, Shuang; Sasaki, Yohei; Zhou, Liming; Matsumura, Hideki; Araki, Shouta; Mezawa, Masaru; Takai, Hideki; Chen, Zhe; Ogata, Yorimasa

doi:10.1016/j.gene.2011.01.016

Cited by 13 publications

(9 citation statements)

References 73 publications

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“…BSP-II is a mineralized tissue-specific protein that is expressed in differentiated osteoblasts and appears to function in the initial formation of apatite crystals and mineralization of bone. 41,42 However, only the NSP-PCL group and not the PCL group exhibited significant increase in Ca 2 + deposition on day 21. The divergence between the two genes that upregulates depending on scaffold type suggests that the morphological structure of the scaffold plays distinctive roles in the differentiation to osteoblasts and subsequent osteogenic response.…”

Section: Discussionmentioning

confidence: 93%

Functionalization of Polycaprolactone Scaffolds with Hyaluronic Acid and β-TCP Facilitates Migration and Osteogenic Differentiation of Human Dental Pulp Stem CellsIn Vitro

Jensen

Kraft

Lysdahl

et al. 2015

Tissue Engineering Part A

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In this study, we sought to assess the osteogenic potential of human dental pulp stem cells (DPSCs) on three different polycaprolactone (PCL) scaffolds. The backbone structure of the scaffolds was manufactured by fused deposition modeling (PCL scaffold). The composition and morphology was functionalized in two of the scaffolds. The first underwent thermal induced phase separation of PCL infused into the pores of the PCL scaffold. This procedure resulted in a highly variable micro- and nanostructured porous (NSP), interconnected, and isotropic tubular morphology (NSP-PCL scaffold). The second scaffold type was functionalized by dip-coating the PCL scaffold with a mixture of hyaluronic acid and β-TCP (HT-PCL scaffold). The scaffolds were cylindrical and measured 5 mm in height and 10 mm in diameter. They were seeded with 1×10(6) human DPSCs, a cell type known to express bone-related markers, differentiate into osteoblasts-like cells, and to produce a mineralized bone-like extracellular matrix. DPSCs were phenotypically characterized by flow cytometry for CD90(+), CD73(+), CD105(+), and CD14(-). DNA, ALP, and Ca(2+) assays and real-time quantitative polymerase chain reaction for genes involved in osteogenic differentiation were analyzed on day 1, 7, 14, and 21. Cell viability and distribution were assessed on day 1, 7, 14, and 21 by fluorescent-, scanning electron-, and confocal microscopy. The results revealed that the DPSCs expressed relevant gene expression consistent with osteogenic differentiation. The NSP-PCL and HT-PCL scaffolds promoted osteogenic differentiation and Ca(2+) deposition after 21 days of cultivation. Different gene expressions associated with mature osteoblasts were upregulated in these two scaffold types, suggesting that the methods in which the scaffolds promote osteogenic differentiation, depends on functionalization approaches. However, only the HT-PCL scaffold was also able to support cell proliferation and cell migration resulting in even cell dispersion throughout the scaffold. In conclusion, DPSCs could be a possible alternate cell source for bone tissue engineering. The HT-PCL scaffold showed promising results in terms of promoting cell migration and osteogenic differentiation, which warrants future in vivo studies.

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Section: Discussionmentioning

confidence: 93%

Functionalization of Polycaprolactone Scaffolds with Hyaluronic Acid and β-TCP Facilitates Migration and Osteogenic Differentiation of Human Dental Pulp Stem CellsIn Vitro

Jensen

Kraft

Lysdahl

et al. 2015

Tissue Engineering Part A

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“…OPN is highly expressed during the last stage of bone formation, namely the mineralization period 50 . ALP is an earlier osteogenic marker that increases rapidly with the proliferation and external secretion of osteoblasts, while BSP has activated the function in the initiation of mineralization, both of which are relatively mature osteogenic differentiation markers 51 . The increased oil red staining and Von Kossa staining in RCCS-3D group as well as the assays of mRNA expressions of adipogenesis-related and osteogenesis-related markers suggested that RCCS-3D system could maintain the potential of adipogenic and osteogenic differentiation when MSCs loading on the collagen 3D scaffolds after a long-term culture, while 2D BMSCs tended to lose their differentiation potential after expanding for same long- term time.…”

Section: Discussionmentioning

confidence: 99%

The combination of three-dimensional and rotary cell culture system promotes the proliferation and maintains the differentiation potential of rat BMSCs

Tang

Xiao

et al. 2017

Sci Rep

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Bone marrow mesenchymal stem cells (BMSCs) are a good candidate for tissue engineering and clinical application. One of the challenges in its cell therapy is how to quickly obtain an adequate number of seed cells and meanwhile maintain suitable differentiation potential. In this study we combined three-dimensional (3D) collagen porous scaffolds with rotary cell culture system (RCCS) (RCCS-3D) to create a stereoscopic dynamic environment for the amplification of rat BMSCs in vitro. The results revealed that this RCCS-3D system could enhance BMSCs’ proliferation and colony formation, as well as maintain the differentiation potential compared with conventional static two-dimensional (2D) and 3D cell culture conditions. In addition, high-throughput microarray analysis showed that gene expressions of RCCS-3D system displayed significant differences in cell proliferation and differentiation compared with static-2D conditions. Thus, RCCS-3D system could provide an effective means for BMSCs cell proliferation in vitro and meanwhile maintain differentiation potential in tissue engineering.

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“…Msx2, Dlx3 and Dlx5 can form co-regulatory complexes with Runx2 that alter the transcription activity of Runx2 [54][55][56]. All of these findings suggest a close relationship between Dlx families and Runx2.…”

Section: Discussionmentioning

confidence: 89%

Insulin-like growth factor-II regulates bone sialoprotein gene transcription

et al. 2015

Self Cite

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Insulin-like growth factor-I and -II (IGF-I and IGF-II) have been found in bone extracts of several different species, and IGF-II is the most abundant growth factor stored in bone. Bone sialoprotein (BSP) is a noncollagenous extracellular matrix glycoprotein associated with mineralized connective tissues. In this study, we have investigated the regulation of BSP transcription by IGF-II in rat osteoblast-like ROS17/2.8 cells. IGF-II (50 ng/ml) increased BSP mRNA and protein levels after 6-h stimulation, and enhanced luciferase activities of the constructs pLUC3 (-116 to +60), pLUC4 (-425 to +60), pLUC5 (-801 to +60) and pLUC6 (-938 to +60). Effects of IGF-II were inhibited by tyrosine kinase, extracellular signal-regulated kinase1/2 and phosphatidylinositol 3-kinase inhibitors, and abrogated by 2-bp mutations in cAMP response element (CRE), FGF2 response element (FRE) and homeodomain protein-binding site (HOX). The results of gel shift assays showed that nuclear proteins binding to CRE, FRE and HOX sites were increased by IGF-II (50 ng/ml) at 3 and 6 h. CREB1, phospho-CREB1, c-Fos and c-Jun antibodies disrupted the formation of the CRE-protein complexes. Dlx5 and Runx2 antibodies disrupted the FRE- and HOX-protein complex formations. These studies therefore demonstrated that IGF-II increased BSP transcription by targeting CRE, FRE and HOX elements in the proximal promoter of the rat BSP gene. Moreover, phospho-CREB1, c-Fos, c-Jun, Dlx5 and Runx2 transcription factors appear to be key regulators of IGF-II effects on BSP transcription.

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Transcriptional regulation of bone sialoprotein gene by interleukin-11

Cited by 13 publications

References 73 publications

Functionalization of Polycaprolactone Scaffolds with Hyaluronic Acid and β-TCP Facilitates Migration and Osteogenic Differentiation of Human Dental Pulp Stem CellsIn Vitro

Functionalization of Polycaprolactone Scaffolds with Hyaluronic Acid and β-TCP Facilitates Migration and Osteogenic Differentiation of Human Dental Pulp Stem CellsIn Vitro

The combination of three-dimensional and rotary cell culture system promotes the proliferation and maintains the differentiation potential of rat BMSCs

Insulin-like growth factor-II regulates bone sialoprotein gene transcription

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