Transcriptional Regulation of Acute Phase Protein Genes

A bridge to silencing: Co-assembling anionic nanoparticles of siRNA and hyaluronan sulfate via calcium ion bridges

“…However, STAT3 silencing induced by HAS-Ca 2+ -siRNA NPs under IL-6 stimulation conditions resulted in a marked decrease in the levels of total and activated STAT3 proteins. Finally, gene expression analysis of the known APR genes downstream to STAT3 (serum amyloid A1 and fibrinogen) [37] demonstrated that the effective silencing of STAT3 suppressed IL-6-induced expression of these known APR markers. Taken together, our data strongly suggest that the developed HAS-based NP delivery platform allows effective and robust gene silencing, suggesting its therapeutic potential as a novel treatment for pathological conditions, where gene silencing could be used to enforce regulation of impaired homeostasis at multiple levels.…”

“…Therefore, we further tested the expression of known downstream APR-related genes, serum amyloid A1 (SAA1) and fibrinogen (Fig. 7D) [31,32,36,37]. IL-6-induced increase in expression levels of SAA1 and fibrinogen, by 22-fold and 15-fold respectively, were detected in non-transfected cells.…”

Modulation of redox-sensitive transcription factors with polyphenols as pathogenetically grounded approach in therapy of systemic inflammatory response

“…A key role of the SIR control is at the level of gene transcription. The coordination of the functions in regulating the inflammatory response is provided by four classes of the TFs [ 46 , 47 ]. constitutively expressed TFs, such as NFκB, STAT, Interferon Regulatory Factors (IRF), activated by signal-dependent post-translational modifications that affect their activation properties and nuclear localization.…”

“…Molecular mechanisms of the inflammatory response transcriptional control have already well-described in the literature [ 47 , 48 ].…”