Transcriptional regulation by folate: inducible gene expression in<i>Dictyostelium</i>transformants during growth and early development

Blusch, Jürgen; Morandini, Piero; Nellen, Wolfgang

doi:10.1093/nar/20.23.6235

Cited by 65 publications

(59 citation statements)

References 24 publications

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“…1B (see Materials and Methods for a description of the other constructs). Two independent constructs for homologous recombination were then linearized and transformed by electroporation into JH10 cells, a thymidine auxotroph (32,56 To further examine the possibility that ERK1 is essential for growth, we made an ERK1 antisense construct with the discoidin I-y (Discly) promoter, whose expression can be regulated by exogenous folate during vegetative growth (3,66). This gene is not expressed in cells grown on bacteria or in axenic culture when cells are maintained at a very low density (15,66).…”

Section: Multicellular Development In Dictyostelium Discoideum Ismentioning

confidence: 99%

“…Expression of the promoter, however, can be repressed in axenic culture (even if the cells are at high density) by the addition of 1 mM folate (3). When the folate is removed, the Discly gene is then expressed (3).…”

Section: Multicellular Development In Dictyostelium Discoideum Ismentioning

confidence: 99%

“…When the folate is removed, the Discly gene is then expressed (3). The DiscIyIERKI antisense construct was transformed into wild-type KAx-3 cells, and G418-resistant transformants were selected in the presence of 1 mM folate.…”

Section: Multicellular Development In Dictyostelium Discoideum Ismentioning

confidence: 99%

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Identification and functional analysis of a developmentally regulated extracellular signal-regulated kinase gene in Dictyostelium discoideum.

Gaskins¹,

Maeda²,

Firtel³

1994

Mol. Cell. Biol.

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We have cloned a developmentally regulated mitogen-activated protein kinase (extracellular signal-regulated kinase) from Dictyostelium discoideum designated ERK1. Using anti-pTyr antibodies, we show that ERKI is phosphorylated on tyrosine in vivo and that it will phosphorylate myelin basic protein. The (16,26). As the aggregate forms, extracellular signals produced within the organism induce cell type differentiation and regulate morphogenesis. These events lead to the production of a migrating slug followed by culmination and the generation of a mature fruiting body containing spores and stalk cells (49,50,80). Biochemical and molecular genetic analyses over the last few years have identified components required for various aspects of these processes and have led to a further understanding of the regulatory pathways controlling development. Genes encoding membrane receptors, coupled GP and Got subunits, the effectors adenylyl cyclase and phospholipase C, and the transcription factor GBF have been cloned and analyzed at a molecular level (12,17,32,65,72,81,82 the membrane to those in the nucleus is cAMP-dependent protein kinase (PKA). PKA has been shown to be essential for aggregation, cell-type-specific expression of the ecmB gene, the induction of prespore cell differentiation, and the induction of sporulation and spore cell-specific gene expression (1, 27, 35, 36, 40, 56-58, 76, 77 (8, 21-23, 61, 79, 84). In metazoans, both G protein-and tyrosine kinase-coupled receptors regulate growth responses, gene expression, and cellular differentiation via MAPK-containing pathways (2,7,11,51,60,64,83). MAPK family members have also been shown to directly phosphorylate transcription factors in mammalian cells, including Myc, Elk, and Jun proteins, leading to an increase in the rate of transcription (14,29,38,46,59,74,75).

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Section: Multicellular Development In Dictyostelium Discoideum Ismentioning

confidence: 99%

Section: Multicellular Development In Dictyostelium Discoideum Ismentioning

confidence: 99%

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Identification and functional analysis of a developmentally regulated extracellular signal-regulated kinase gene in Dictyostelium discoideum.

Gaskins¹,

Maeda²,

Firtel³

1994

Mol. Cell. Biol.

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“…However, these attempts failed, suggesting that rapA might be an essential gene in Dictyostelium. In the present study, we have expressed a rapA antisense construct under the control of the folate repressible discoidin promoter (Blusch et al, 1992) and have examined the effects of Rap1 depletion on cell function. We have also demonstrated that Rap1 is activated in response to hyperosmotic stress and low temperature.…”

Section: Introductionmentioning

confidence: 99%

Evidence for a role for the Dictyostelium Rap1 in cell viability and the response to osmotic stress

Kang

Kae

et al. 2002

Journal of Cell Science

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The Dictyostelium genome contains a single rapA gene,which encodes a Rap1 monomeric G protein. As attempts at generating rapA-null Dictyostelium cells had been unsuccessful,expression of antisense RNA from the rapA gene under control of the folate repressible discoidin promoter was used to reduce cellular levels of the Rap1 protein. As Rap1 levels gradually decreased following antisense rapA RNA induction, growth rate and cell viability also decreased, a result consistent with the idea that rapA is an essential gene. The Rap1-depleted cells exhibited reduced viability in response to osmotic shock. The accumulation of cGMP in response to 0.4 M sorbitol was reduced after rapA antisense RNA induction and was enhanced in cells expressing the constitutively activated Rap1(G12V) protein, suggesting a role for Rap1 in the generation of cGMP. Dictyostelium Rap1 formed a complex with the Ras-binding domain of RalGDS only when it was in a GTP-bound state. This assay was used to demonstrate that activation of Rap1 in response to 0.4 M sorbitol occurred with initial kinetics similar to those observed for the accumulation of cGMP. Furthermore, the addition of 2 mM EDTA to osmotically shocked cells, a treatment that enhances cGMP accumulation, also enhanced Rap1 activation. These results suggest a direct role for Rap1 in the activation of guanylyl cyclase during the response to hyperosmotic conditions. Rap1 was also activated in response to low temperature but not in response to low osmolarity or high temperature.

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“…Different sequence elements within the discoidin promoter were responsible for repression by CAMP and folate , respectively. Folate may be important in the development of Dictyostelium cells as a singal molecule for the presence of bacteria on which this organism feeds (89) .…”

Section: Eukaryotesmentioning

confidence: 99%

Regulation of Gene Expression by Cofactors Derived from B Vitamins.

Brandsch

1994

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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SummaryVitamins represent precursor molecules for the majority of cofactors essential for various enzyme activities. Is the expression of proteins that require a specific cofactor for their function correlated to its availability? Examples are presented and discussed of the involvement of vitamin-derived cofactors in the regulatory mechanisms controlling gene expression in bacteria and eukaryotes at the transcriptional and trans lational level.

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Transcriptional regulation by folate: inducible gene expression inDictyosteliumtransformants during growth and early development

Cited by 65 publications

References 24 publications

Identification and functional analysis of a developmentally regulated extracellular signal-regulated kinase gene in Dictyostelium discoideum.

Identification and functional analysis of a developmentally regulated extracellular signal-regulated kinase gene in Dictyostelium discoideum.

Evidence for a role for the Dictyostelium Rap1 in cell viability and the response to osmotic stress

Regulation of Gene Expression by Cofactors Derived from B Vitamins.

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