Transcriptional program controlled by the floral homeotic gene<i>AGAMOUS</i>during early organogenesis

Gómez‐Mena, Concepción; Folter, Stefan de; Costa, Maria Manuela Ribeiro; Angenent, Gerco C.; Sablowski, Robert

doi:10.1242/dev.01600

Cited by 318 publications

(285 citation statements)

References 72 publications

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“…1C; Supplemental Table S2), suggesting that the complement of stamen-expressed transcripts is considerably different at distinct stages of flower development. This idea is in agreement with the recent finding that the transcriptome of young floral buds shows only a limited overlap with that of more mature flowers (Gomez-Mena et al, 2005;Wellmer et al, 2006), as well as with the observation of substantial transcriptional changes during the progression from proliferating microspores to differentiated pollen (Honys and Twell, 2004).…”

Section: Genome-wide Analysis Of Gene Expression During Stamen Develosupporting

confidence: 91%

“…2J). For instance, the genes At3g17010 (a likely target of the floral homeotic factor AGAMOUS; Gomez-Mena et al, 2005) and At5g09780, which encode putative B3-type transcription factors, were identified in the ap3 es but not in the in spl/nzz or ms1 experiments (Fig. 1D, cluster 19), suggesting expression at early stages of stamen development before the formation of sporogenous tissues.…”

Section: Genome-wide Analysis Of Gene Expression During Stamen Develomentioning

confidence: 99%

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Global Expression Profiling Applied to the Analysis of Arabidopsis Stamen Development

et al. 2007

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To obtain detailed information about gene expression during stamen development in Arabidopsis (Arabidopsis thaliana), we compared, by microarray analysis, the gene expression profile of wild-type inflorescences to those of the floral mutants apetala3, sporocyteless/nozzle, and male sterile1 (ms1), in which different aspects of stamen formation are disrupted. These experiments led to the identification of groups of genes with predicted expression at early, intermediate, and late stages of stamen development. Validation experiments using in situ hybridization confirmed the predicted expression patterns. Additional experiments aimed at characterizing gene expression specifically during microspore formation. To this end, we compared the gene expression profiles of wild-type flowers of distinct developmental stages to those of the ms1 mutant. Computational analysis of the datasets derived from this experiment led to the identification of genes that are likely involved in the control of key developmental processes during microsporogenesis. We also identified a large number of genes whose expression is prolonged in ms1 mutant flowers compared to the wild type. This result suggests that MS1, which encodes a putative transcriptional regulator, is involved in the stage-specific repression of these genes. Lastly, we applied reverse genetics to characterize several of the genes identified in the microarray experiments and uncovered novel regulators of microsporogenesis, including the transcription factor MYB99 and a putative phosphatidylinositol 4-kinase.

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Section: Genome-wide Analysis Of Gene Expression During Stamen Develosupporting

confidence: 91%

Section: Genome-wide Analysis Of Gene Expression During Stamen Develomentioning

confidence: 99%

Global Expression Profiling Applied to the Analysis of Arabidopsis Stamen Development

et al. 2007

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“…AG promotes FM termination by indirectly repressing WUS (Sieburth et al, 1998;Lenhard et al, 2001;Lohmann et al, 2001) but no intermediate between AG and WUS has been found so far. However, GA4, a gene involved in the biosynthesis of gibberellins, has recently been identified among the targets of AG (Gomez-Mena et al, 2005). Gibberellins oppose meristem activity (for review, see Shani et al, 2006), raising the possibility that AG promotes FM termination by modifying phytohormone levels within the FM (discussed by Sablowski, 2007b).…”

Section: Resultsmentioning

confidence: 99%

“…CRC expression is directly activated by AG (Bowman and Smyth, 1999;Gomez-Mena et al, 2005). Therefore, part of AG function in promoting carpel development and FM termination could be mediated by the downstream action of CRC.…”

Section: Crc a Gene Involved In The Female Program Also Controls Fmmentioning

confidence: 99%

“…Therefore, part of AG function in promoting carpel development and FM termination could be mediated by the downstream action of CRC. The possibility that CRC could in turn activate AG in a positive feedback was suggested (Gomez-Mena et al, 2005), but no direct evidence supports it so far. Indeed, CRC is expressed in floral organs in the absence of AG when AP2 is also mutated, and confers on them some carpelloid features, showing that both CRC activation and function are at least partially independent of AG (Alvarez and Smyth, 1999;Bowman and Smyth, 1999).…”

Section: Crc a Gene Involved In The Female Program Also Controls Fmmentioning

confidence: 99%

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Time to Stop: Flower Meristem Termination

Prunet¹,

Morel²,

Negrutiu³

et al. 2009

Plant Physiol.

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Arabidopsis: Flower Development and Patterning

Krizek

2015

Encyclopedia of Life Sciences

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The development of flowers and floral organs is directed by intricate genetic programmes, many aspects of which appear to be shared among angiosperms. Early acting genes establish floral meristem identity in flower primordia initiated at the periphery of the inflorescence meristem. Later, floral organ primordia arise at precise positions within these floral meristems and take on one of the four distinct identities (sepals, petals, stamens and carpels). The ABCE model, supported by both molecular and genetic experiments in Arabidopsis , explains how a small number of regulatory genes (called floral homeotic genes or floral organ identity genes) act in different combinations to specify these different organ types. The floral organ identity genes encode transcription factors that form distinct higher order protein complexes in different regions of a flower primordium to control the expression of target genes responsible for organogenesis. Key Concepts Lateral organs produced by the shoot apical meristem during reproductive development acquire their identity as flowers through the action of floral meristem identity genes such as LEAFY and APETALA1 . The identities of each of the four organ types of a flower (sepal, petal, stamen and carpel) is conferred by a unique combination of floral organ identity gene activities, referred to as class A, B, C and E in the ABCE model. The activities of the class A, B and C genes are restricted to particular regions within a developing flower primarily, but not exclusively, through transcriptional regulation. The MADS domain transcription factors encoded by the class A, B, C and E genes form unique tetrameric transcriptional regulatory complexes in cells of each floral whorl. The transcriptional regulatory complexes formed by the A, B, C and E proteins regulate distinct sets of genes at different stages of flower development. Many aspects of the genetic programmes conferring floral meristem identity and floral organ identity are conserved among all angiosperms.

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Transcriptional program controlled by the floral homeotic geneAGAMOUSduring early organogenesis

Cited by 318 publications

References 72 publications

Global Expression Profiling Applied to the Analysis of Arabidopsis Stamen Development

Global Expression Profiling Applied to the Analysis of Arabidopsis Stamen Development

Time to Stop: Flower Meristem Termination

Arabidopsis: Flower Development and Patterning

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