Transcriptional profiling to address molecular determinants of endometrial receptivity – Lessons from studies in livestock species

Ulbrich, Susanne E.; Groebner, Anna E.; Bauersachs, Stefan

doi:10.1016/j.ymeth.2012.10.013

Cited by 38 publications

(27 citation statements)

References 102 publications

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“…Factors from the endometrium may also stimulate the expression of IFNT in the conceptus trophectoderm (Roberts et al 2003 ;Michael et al 2006 ;Ealy and Yang 2009 ;Kim et al 2011 ). Several recent reviews catalogue the endometrial contributions to uterine luminal fl uid that functions in conceptus elongation in ruminants (Roberts et al 2008 ;Spencer et al 2008 ;Bazer et al 2010 ;Forde and Lonergan 2012 ;Bauersachs and Wolf 2013 ;Dorniak et al 2013 ;Ulbrich et al 2013 ;Lonergan and Forde 2014 ).…”

Section: Embryotrophic Factors In the Uterine Lumen Regulating Concepmentioning

confidence: 99%

Implantation and Establishment of Pregnancy in Ruminants

Spencer

Hansen

2015

Advances in Anatomy, Embryology and Cell Biology

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The establishment of pregnancy in ruminants occurs during the peri-implantation period and involves the suppression of the endometrial luteolytic mechanism to maintain progesterone production by the corpus luteum (CL). Reciprocal interactions between the elongating conceptus (embryo/fetus and associated extraembryonic membranes) and endometrium culminate in implantation. Antiluteolytic effects of the conceptus are due to the production of interferon tau (IFNT) by the trophoblast that has a paracrine effect to inhibit the upregulation of oxytocin receptors in the endometrial epithelia, thereby disrupting uterine release of luteolytic prostaglandin F2 alpha (PGF) pulses. Additionally, IFNT is released into the uterine vein and has endocrine actions to induce ISGs in peripheral tissues. For example, IFNT may induce luteal resistance to PGF, thereby ensuring survival of the CL and maintenance of pregnancy. Survival of the blastocyst and elongation of the conceptus requires embryotrophic factors from the epithelia of the uterus, and those embryotrophic factors are regulated by ovarian progesterone as well as conceptus-derived factors including IFNT and prostaglandins. This review provides new concepts on mechanisms of the establishment of pregnancy and implantation in ruminants with emphasis on conceptus-maternal signaling associated with elongation of the blastocyst and endometrial responses to the presence of a conceptus.

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Section: Embryotrophic Factors In the Uterine Lumen Regulating Concepmentioning

confidence: 99%

Implantation and Establishment of Pregnancy in Ruminants

Spencer

Hansen

2015

Advances in Anatomy, Embryology and Cell Biology

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“…Most of these studies investigate the transcriptomic signature in the whole endometrial tissue without separating the different compartments. However, in some studies, laser capture micro-dissection has facilitated specific compartment gene expression profiles (Yanaihara et al 2005;Evans et al 2012;Ulbrich et al 2013). Even the specific profiles for stromal cells and glands at different depths in the endometrium have been reported (Gaide Chevronnay et al 2009).…”

Section: Transcriptomics Of the Endometriummentioning

confidence: 99%

Human Endometrial Transcriptomics: Implications for Embryonic Implantation

Gómez

Ruiz-Alonso

Miravet

et al. 2015

Cold Spring Harb Perspect Med

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Human endometrium has been extensively investigated in the search for markers capable of predicting its receptive status. The completion of the Human Genome Project has triggered a rapid development of new fields in molecular biology, the "transcriptomics" being a major turning point in the knowledge acquisition of endometrial receptivity. Based on this, a customized Endometrial Receptivity Array (ERA) has been developed, which is capable of identifying the genomic signature of receptivity. This diagnostic tool showed that the window of implantation (WOI) is displaced in one out of four patients with implantation failure, allowing the identification of their personalized WOI. This strategy allows performing a personalized embryo transfer ( pET) on the day in which the endometrium is receptive. The combination of a systems biology approach and next-generation sequencing will overcome the limitations of microarrays, and will, in the future, allow elucidation of the mechanisms involved in embryo implantation.T he endometrium, which lines the inside of the uterine cavity, undergoes cyclic changes that are regulated by ovarian steroids. It can be subdivided into the basal layer that is responsible for its regenerative capacity, and the functional layer that undergoes proliferation, secretion, and tissue degeneration every month from menarche to menopause. Its aim is to prepare the optimal moment for embryonic implantation known as the window of implantation (WOI).The endometrial cycle comprises the menstrual, proliferative, and secretory phases. The proliferative phase, which corresponds to the ovarian follicular phase and increased production of estrogens, lasts until ovulation occurs. During this stage, the increasing estrogen levels cause the proliferation of the stromal cells as well as the elongation of the spiral arteries. After ovulation, with the appearance of progesterone secreted by the granulosa-luteal cells, the secretory phase begins. If implantation does not occur, the secretory phase ends, and the corpus luteum degenerates. Menstruation occurs owing to the drop of estrogen and progesterone, which resets the endometrium until pregnancy

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“…Altogether, these findings make this phase particularly interesting for studying uterine receptivity and embryo-maternal crosstalk. In addition to numerous studies focusing on individual candidate genes or major players in involved signaling pathways (reviewed in References 20,21), several global approaches have been applied in livestock species to get an overview of endometrial gene expression changes during the preimplantation phase (22)(23)(24)(25)(26)(27). Endometrial samples collected on selected days of the preimplantation phase and during the estrous cycle were analyzed with DNA microarrays and by RNA sequencing (RNA-seq) to study gene expression changes associated with recognition of pregnancy, embryo implantation, and uterine receptivity.…”

Section: Introductionmentioning

confidence: 99%

Uterine Responses to the Preattachment Embryo in Domestic Ungulates: Recognition of Pregnancy and Preparation for Implantation

Bauersachs

Wolf²

2015

Annu. Rev. Anim. Biosci.

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The endometrium is a tissue newly evolved with the development of mammalian species. Its main function is the support of embryonic growth and development and the nutrition of the fetus. The species-specific differences in establishment and maintenance of pregnancy make the study of this tissue in various mammalian organisms particularly interesting. With the application of omics technologies to various mammalian species, many systematic studies of endometrial gene expression changes during the phase of establishment of pregnancy have been performed to obtain a global view of regulatory events associated with this biological process. This review summarizes the results of trancriptome studies of bovine, porcine, and equine endometrium. Furthermore, the results are compared between these species and to humans. Because an increasing number of studies suggest an important role of small regulatory RNAs (i.e., microRNAs), recent findings related to the regulation of endometrial functions and the development of the conceptus are presented.

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Transcriptional profiling to address molecular determinants of endometrial receptivity – Lessons from studies in livestock species

Cited by 38 publications

References 102 publications

Implantation and Establishment of Pregnancy in Ruminants

Implantation and Establishment of Pregnancy in Ruminants

Human Endometrial Transcriptomics: Implications for Embryonic Implantation

Uterine Responses to the Preattachment Embryo in Domestic Ungulates: Recognition of Pregnancy and Preparation for Implantation

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