Transcriptional profiling of drug-induced liver injury biomarkers: association of hepatic Srebf1/Pparα signaling and crosstalk of thrombin, alcohol dehydrogenase, MDR and DNA damage regulators

Ghanim, Bayan Y.; Ahmad, Mohammad I.; Abdallah, Qasem; Khaleel, Anas; Qinna, Nidal A.

doi:10.1007/s11010-022-04648-1

Cited by 1 publication

(2 citation statements)

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“…However, it is also reported that upregulation of FASN in hepatocytes can be considered as a compensatory adaptation in the early stages of the development of toxic liver damage [64]. Furthermore, the level of expression of FASN has been found to increase when exposed to ethanol and CCl4, which is in complete agreement with our data [65].…”

Section: Real-time Pcrsupporting

confidence: 92%

“…In the ethanol and APAP models, expression did not differ from the controls at 48 h (Figure 6). Numerous works describe a decrease in the expression of FASN; in particular, FASN was downregulated in the livers of mice receiving 70 mg/kg APAP due to oxidative stress, which inhibits fatty acid synthesis [65]. However, it is also reported that upregulation of FASN in hepatocytes can be considered as a compensatory adaptation in the early stages of the development of toxic liver damage [64].…”

Section: Real-time Pcrmentioning

confidence: 99%

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Label-Free Imaging Techniques to Evaluate Metabolic Changes Caused by Toxic Liver Injury in PCLS

Rodimova

Mozherov

Elagin

et al. 2023

IJMS

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Abuse with hepatotoxic agents is a major cause of acute liver failure. The search for new criteria indicating the acute or chronic pathological processes is still a challenging issue that requires the selection of effective tools and research models. Multiphoton microscopy with second harmonic generation (SHG) and fluorescence lifetime imaging microscopy (FLIM) are modern label-free methods of optical biomedical imaging for assessing the metabolic state of hepatocytes, therefore reflecting the functional state of the liver tissue. The aim of this work was to identify characteristic changes in the metabolic state of hepatocytes in precision-cut liver slices (PCLSs) under toxic damage by some of the most common toxins: ethanol, carbon tetrachloride (CCl4) and acetaminophen (APAP), commonly known as paracetamol. We have determined characteristic optical criteria for toxic liver damage, and these turn out to be specific for each toxic agent, reflecting the underlying pathological mechanisms of toxicity. The results obtained are consistent with standard methods of molecular and morphological analysis. Thus, our approach, based on optical biomedical imaging, is effective for intravital monitoring of the state of liver tissue in the case of toxic damage or even in cases of acute liver injury.

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Section: Real-time Pcrsupporting

confidence: 92%

Section: Real-time Pcrmentioning

confidence: 99%