Transcriptional profiling of buffalo mammary gland with different milk fat contents

Hao, Meilin; Jiang, Juncai; Zhang, Yongyun; Wang, Shaoqing; Fu, Guowen; Zou, Feng-Cai; Xie, Yong; Zhao, Sumei; Li, Weizhen

doi:10.1016/j.gene.2021.145864

Cited by 4 publications

(4 citation statements)

References 49 publications

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“…In addition, lipid droplets are involved in cellular lipid synthesis, metabolism, transport, storage of substances, and cell signaling processes . Water buffalo milk has higher nutritional value in fat, protein, and iron content compared to dairy cow milk . In the current study, more intense lipid droplet aggregation was visible in BufMECs compared to BovMECs as judged by staining with the fluorescent neutral lipid dye BODIPY, which supported that buffalo milk has higher fat contents compared to dairy cow milk.…”

Section: Discussionsupporting

confidence: 76%

“…39 Water buffalo milk has higher nutritional value in fat, protein, and iron content compared to dairy cow milk. 40 In the current study, more intense lipid droplet aggregation was visible in BufMECs compared to BovMECs as judged by staining with the fluorescent neutral lipid dye BODIPY, which supported that buffalo milk has higher fat contents compared to dairy cow milk. ACAA1 is an important regulator of lipid metabolism, and its upregulation was involved in fatty acid biosynthesis in beef cattle.…”

Section: ■ Discussionsupporting

confidence: 76%

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Comparative Genomic Analysis of the Thiolase Family and Functional Characterization of the Acetyl-Coenzyme A Acyltransferase-1 Gene for Milk Biosynthesis and Production of Buffalo and Cattle

Deng

Abdel-Shafy

et al. 2023

J. Agric. Food Chem.

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Cattle and buffalo served as the first and second largest dairy animals, respectively, providing 96% milk products worldwide. Understanding the mechanisms underlying milk synthesis is critical to develop the technique to improve milk production. Thiolases, also known as acetyl-coenzyme A acetyltransferases (ACAT), are an enzyme family that plays vital roles in lipid metabolism, including ACAT1, ACAT2, ACAA1, ACAA2, and HADHB. Our present study showed that these five members were orthologous in six livestock species including buffalo and cattle. Transcriptomic data analyses derived from different lactations stages showed that ACAA1 displayed different expression patterns between buffalo and cattle. Immunohistochemistry staining revealed that ACAA1 were dominantly located in the mammary epithelial cells of these two dairy animals. Knockdown of ACAA1 inhibited mammary epithelial cell proliferation and triglyceride and β-casein secretion by regulating related gene expressions in cattle and buffalo. In contrast, ACAA1 overexpression promoted cell proliferation and triglyceride secretion. Finally, three novel SNPs (g.-681A>T, g.-23117C>T, and g.-24348G>T) were detected and showed significant association with milk production traits of Mediterranean buffaloes. In addition, g.-681A>T mutation located in the promoter region changed transcriptional activity significantly. Our findings suggested that ACAA1 play a key role in regulating buffalo and cattle milk synthesis and provided basic information to further understand the dairy animal lactation physiology.

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Section: Discussionsupporting

confidence: 76%

Section: ■ Discussionsupporting

confidence: 76%

Comparative Genomic Analysis of the Thiolase Family and Functional Characterization of the Acetyl-Coenzyme A Acyltransferase-1 Gene for Milk Biosynthesis and Production of Buffalo and Cattle

Deng

Abdel-Shafy

et al. 2023

J. Agric. Food Chem.

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“…Omics-based studies have also been used to investigate milk production. Transcriptomics showed that differential expression of genes involved in pathways for milk protein, fat synthesis and the immune response influenced buffalo milk fat content [58]. Metabolomics identified breed-specific metabolic markers for milk quality that could be used to produce buffalo with high milk fat [59].…”

Section: Advances In Molecular Breeding Of China Dairy Buffalomentioning

confidence: 99%

Genetic Bases and Molecular Breeding of Key Economic Traits in China Dairy Cattle: A Progress Report

Ma¹,

Li²,

Xue³

et al. 2022

IJCCR

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Animal growth is a coordinated developmental process that requires altering the expression of hundreds to thousands of genes to modify many biochemical and biological signaling cascades. The genetic analysis of economic traits and molecular breeding research are hotspots for animal husbandry and, in the case of Chinese dairy cattle, much progress has been made in recent years. Actually, the level of information of molecular events at the transcriptional, biochemical, hormonal, and metabolite levels underlying animal development process has increased considerably. The current review summarizes progress in research into the genetic basis of economic traits and molecular breeding of dairy cows, dual-purpose cattle and dairy buffalo, to better understand the molecular switch involved in development process with important consequences from a breeding point of view.

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“…However, [9] report that in poor nations, the quality of water buffalo milk is solely determined by its protein and fat content. As the main source of energy in milk, milk fat is crucial to the product's overall quality and health benefits [10]. Breeds, genetic information, environmental factors, and management techniques all have an impact on milk yield and composition [11].…”

Section: Introductionmentioning

confidence: 99%

Investigation of Polymorphic Variations in the Alpha-Lactalbumin Gene and their Association Analysis to Milk Characteristics in River Buffalo

Sihag,

Kumar,

Jangra

et al. 2023

CJAST

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River buffalo (Bubalus bubalis) is one of the most important livestock species in many poor countries because of its invaluable contributions to numerous agricultural fields. Detection of single nucleotide polymorphisms (SNPs) in exon 1 of alpha-lactalbumin (ALA) gene was the main goal of the current study. Polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) assay and sequencing analysis were applied to identify segregated SNPs in ALA gene. Potential SNPs in ALA gene have been identified in the present study. The homozygous genotype (AA) of the ALA gene was significantly associated with fat percentage. Moreover, the heterozygous genotype (LA) revealed significant association with protein percentage, and fat yield. The results obtained in this study emphasize the necessity of screening a broader number of buffaloes distributed in varied environments to identify genetic polymorphisms and their potential correlation with milk production traits.

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Transcriptional profiling of buffalo mammary gland with different milk fat contents

Cited by 4 publications

References 49 publications

Comparative Genomic Analysis of the Thiolase Family and Functional Characterization of the Acetyl-Coenzyme A Acyltransferase-1 Gene for Milk Biosynthesis and Production of Buffalo and Cattle

Comparative Genomic Analysis of the Thiolase Family and Functional Characterization of the Acetyl-Coenzyme A Acyltransferase-1 Gene for Milk Biosynthesis and Production of Buffalo and Cattle

Genetic Bases and Molecular Breeding of Key Economic Traits in China Dairy Cattle: A Progress Report

Investigation of Polymorphic Variations in the Alpha-Lactalbumin Gene and their Association Analysis to Milk Characteristics in River Buffalo

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