Transcriptional profiling of Arabidopsis seedlings in response to heavy metal lead (Pb)

Li, Shipeng; Liu, Shuiying; Guan, Hua; Ma, Ligeng; Chen, Zhangliang; Gu, Hongya; Liu, Qu

doi:10.1016/j.envexpbot.2009.03.016

Cited by 94 publications

(55 citation statements)

References 46 publications

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“…As stated in detail in the Introduction, some of the main toxic effects of Pb are derived from its capacity to stimulate ROS production (Sharma and Dietz, 2008), while others depend on interaction of the metal with the SH groups of essential macromolecules (Liu et al, 2009). Accordingly, antioxidant enzymes such as GR, SOD, APx, and CAT, as well as repair proteins with chaperone functions are the main components of "second line" defence mechanisms against Pb.…”

Section: Discussionmentioning

confidence: 99%

“…Irreversible binding of Pb with the RuBisCO enzyme dissociates the latter into its component subunits, leading to a loss of activity. In other cases, Pb affects enzyme activities, often towards inhibitory effects, either directly, by interacting with SH groups, or indirectly, via Pb-induced ROS, which in turn oxidizes the SH groups of proteins (Liu et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Different strategies to achieve Pb-tolerance by the two Trebouxia algae coexisting in the lichen Ramalina farinacea

Álvarez

Hoyo

García‐Breijo

et al. 2012

Journal of Plant Physiology

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Elsevier Álvarez, R.; del Hoyo, A.; García Breijo, FJ.; Reig Armiñana, J.; Del Campo, E.; Guera, A.; Casano, L. (2012). Different strategies to achieve Pb-tolerance by the two Trebouxia algae coexisting in the lichen Ramalina farinacea. Lichen thalli are permeable to airborne substances, including heavy metals, which are harmful to cell metabolism. Ramalina farinacea shows a moderate tolerance to Pb. This lichen comprises two Trebouxia phycobionts, provisionally referred to as TR1 and TR9, with distinct physiological responses to acute oxidative stress. Thus, there is a more severe decay in photosynthesis and photosynthetic pigments in TR1 than in TR9. Similarly, under oxidative stress, antioxidant enzymes and HSP70 protein decrease in TR1 but increase in TR9. Since Pb toxicity is associated with increased ROS formation, we hypothesized greater Pb tolerance in this phycobiont. Accordingly, the aim of the present study was to characterize the physiological differences in the responses of TR1 and TR9 to Pb exposure. Liquid cultures of isolated phycobionts were incubated for 7 days in the presence of Pb(NO 3 ) 2 . Thereafter, extracellular and intracellular Pb accumulation, photosynthetic pigments, and photosynthesis (as modulated chlorophyll fluorescence) were analyzed along with the antioxidant enzymes glutathione reductase (GR), superoxide dismutase (SOD), ascorbate peroxidase (APx), and catalase (CAT), and the stress-related protein HSP70. Pb uptake increased with the amount of supplied Pb in both algae. However, while significantly more metal was immobilized extracellularly by TR9, the amount of intracellular Pb accumulation was three times higher in TR1. In neither of the phycobionts were significant effects on photosynthetic pigments or photosynthetic electron transport observed. While under control conditions GR, SOD, and APx levels were significantly higher in TR1 than in TR9, only in the latter were these enzymes induced by Pb. This resulted in quantitatively similar antioxidant activities in the two algae when exposed to Pb. In conclusion, the phycobionts of R. farinacea make use of two different strategies against stress, in which the integration of distinct anatomical and physiological features affords similar levels of Pb tolerance.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Different strategies to achieve Pb-tolerance by the two Trebouxia algae coexisting in the lichen Ramalina farinacea

Álvarez

Hoyo

García‐Breijo

et al. 2012

Journal of Plant Physiology

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“…In the Iberian Peninsula, it occurs at all altitudes, more frequently in areas with regular foggy weather, showing preference for places with high atmospheric humidity. This lichen can be found in polluted and unpolluted areas [16,28,29] indicating, at least, a moderate-high tolerance to Pb, as suggested by previous experiments in the laboratory [8]. Recently, we have demonstrated that each thallus of R. farinacea contains not just one, but two Trebouxia phycobionts (provisionally named TR1 and TR9).…”

Section: Introductionmentioning

confidence: 70%

Lichen Rehydration in Heavy Metal-Polluted Environments: Pb Modulates the Oxidative Response of Both Ramalina farinacea Thalli and Its Isolated Microalgae

et al. 2014

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Lichens are adapted to desiccation/rehydration and accumulate heavy metals, which induce ROS especially from the photobiont photosynthetic pigments. Although their mechanisms of abiotic stress tolerance are still to be unravelled, they seem related to symbionts' reciprocal upregulation of antioxidant systems. With the aim to study the effect of Pb on oxidative status during rehydration, the kinetics of intracellular ROS, lipid peroxidation and chlorophyll autofluorescence of whole Ramalina farinacea thalli and its isolated microalgae (Trebouxia TR1 and T. TR9) was recorded. A genetic characterization of the microalgae present in the thalli used was also carried out in order to assess possible correlations among the relative abundance of each phycobiont, their individual physiological responses and that of the entire thallus. Unexpectedly, Pb decreased ROS and lipid peroxidation in thalli and its phycobionts, associated with a lower chlorophyll autofluorescence. Each phycobiont showed a particular pattern, but the oxidative response of the thallus paralleled the TR1's, agreeing with the genetic identification of this strain as the predominant phycobiont. We conclude that: (1) the lichen oxidative behaviour seems to be modulated by the predominant phycobiont and (2) Pb evokes in R. farinacea and its phycobionts strong mechanisms to neutralize its own oxidant effects along with those of rehydration.

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“…The deleterious effects resulting from Pb exposure can also be observed in plants as a consequence of disturbance in the uptake and/or distribution of other essential elements. [14] In addition, ROS can be formed as a result of Pb stress, causing oxidative damages in cells. [14,15].…”

Section: Introductionmentioning

confidence: 99%

“…[14] In addition, ROS can be formed as a result of Pb stress, causing oxidative damages in cells. [14,15]. Secale cereale (rye), belonging to the Triticaceae family, is a wild species mainly distributed in central and eastern Turkey, as well as in neighbouring regions.…”

Section: Introductionmentioning

confidence: 99%

Screening of damage induced by lead (Pb) in rye (Secale cereale L.) – a genetic and physiological approach

Özyiğit

Doğan

Igdelioglu

et al. 2016

Biotechnology & Biotechnological Equipment

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The fields in which lead (Pb) finds application in the modern world have increased dramatically in recent years. As a consequence of this intensive utilization of Pb, its toxicity tends to pose more and more environmental problems. The aim of this study was to evaluate the genotoxic potential of Pb and to characterize some physiological parameters in Secale cereale under Pb stress. Plants were subjected to different exposure levels of Pb (0, 100, 200 and 400 mmol/L) for two weeks. At the end of the experimental period, the effects of Pb exposure on the photosynthetic pigments content (chlorophyll a and b, total chlorophyll, chlorophyll a/b and carotenoids) and genetic material of S. cereale were studied. To evaluate the genotoxic effect of Pb, random amplified polymorphic DNA À polymerase chain reaction (RAPD-PCR) was employed. The obtained results showed alteration in the photosynthetic pigments content and RAPD-PCR profiles of S. cereale grown in the presence of Pb. The alterations in the RAPD-PCR profiles following Pb treatments appeared to be losses of normal bands and occurrences of new bands compared to unexposed plantlets. Overall, the content of chlorophyll a, chlorophyll b, total chlorophyll and carotenoids decreased by 6.68%, 6.08%, 2.89% and 8.57%, respectively, under severe Pb stress (400 mmol/L).

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Transcriptional profiling of Arabidopsis seedlings in response to heavy metal lead (Pb)

Cited by 94 publications

References 46 publications

Different strategies to achieve Pb-tolerance by the two Trebouxia algae coexisting in the lichen Ramalina farinacea

Different strategies to achieve Pb-tolerance by the two Trebouxia algae coexisting in the lichen Ramalina farinacea

Lichen Rehydration in Heavy Metal-Polluted Environments: Pb Modulates the Oxidative Response of Both Ramalina farinacea Thalli and Its Isolated Microalgae

Screening of damage induced by lead (Pb) in rye (Secale cereale L.) – a genetic and physiological approach

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