Transcriptional profiling analysis of susceptible and resistant strains of Anticarsia gemmatalis and their response to Bacillus thuringiensis

Pezenti, Larissa Forim; Sosa–Gómez, Daniel Ricardo; Souza, Rui Fragassi; Vilas-Bôas, Laurival A.; Gonçalves, Kátia B.; Silva, Carlos Roberto Maximiano da; Vilas-Bôas, Gislayne Trindade; Baranoski, Adrivânio; Mantovani, Mário; Rosa, Renata da

doi:10.1016/j.ygeno.2021.05.012

Cited by 8 publications

(7 citation statements)

References 55 publications

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“…It was reported that the expression of Serpin and PAP s was significantly affected by Bt HD73 in P. xylostella [ 112 ]. Serine proteases and serine protease inhibitors are also highly regulated in Bt -susceptible and -resistant Anticarsia gemmatalis strains in response to Bt [ 113 ]. In the midgut of P. xylostella larvae, the PPO cascade is induced after infection with Bt 8010 (the Bt strain) [ 66 ].…”

Section: Immunity Induced By Btmentioning

confidence: 99%

“…In addition to acting as a PGRP in pathogen recognition, lectin interacts with Bt proteins. Some members of the lectin family were upregulated after feeding Bt [ 113 , 140 ], thereby diminishing the toxicity in many insects and causing resistance to Bt toxin by forming cell aggregates. C-type lectin-20 interacts with alkaline phosphatase 1 (ALP1, a receptor of Bt toxins), decreasing the insecticidal activity of Cry toxin in Aedes aegypti [ 141 ].…”

Section: Immunity Induced By Btmentioning

confidence: 99%

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Involvement of an Enhanced Immunity Mechanism in the Resistance to Bacillus thuringiensis in Lepidopteran Pests

Xiao

Yao

Bai

et al. 2023

Insects

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Bacillus thuringiensis (Bt) is the safest, economically successful entomopathogen to date. It is extensively produced in transgenic crops or used in spray formulations to control Lepidopteran pests. The most serious threat to the sustainable usage of Bt is insect resistance. The resistance mechanisms to Bt toxins depend not only on alterations in insect receptors, but also on the enhancement of insect immune responses. In this work, we review the current knowledge of the immune response and resistance of insects to Bt formulations and Bt proteins, mainly in Lepidopteran pests. We discuss the pattern recognition proteins for recognizing Bt, antimicrobial peptides (AMPs) and their synthetic signaling pathways, the prophenoloxidase system, reactive oxygen species (ROS) generation, nodulation, encapsulation, phagocytosis, and cell-free aggregates, which are involved in immune response reactions or resistance to Bt. This review also analyzes immune priming, which contributes to the evolution of insect resistance to Bt, and puts forward strategies to improve the insecticidal activity of Bt formulations and manage insect resistance, targeting the insect immune responses and resistance.

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Section: Immunity Induced By Btmentioning

confidence: 99%

Section: Immunity Induced By Btmentioning

confidence: 99%

Involvement of an Enhanced Immunity Mechanism in the Resistance to Bacillus thuringiensis in Lepidopteran Pests

Xiao

Yao

Bai

et al. 2023

Insects

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“…The specimens of A. gemmatalis were collected in Sertanopolis, State of Paraná, Brazil (23°03′50″ S, 51°00′50″ W). The identification, maintenance, and selection of Bt susceptible and resistant specimens was performed as described in Pezenti et al [2021]. After selection procedures, resistant and susceptible third instar larvae (at 242nd generation with resistance ratio = 133) were fed for 24 h on a diet containing Bt strain HD73 [Pezenti et al, 2021].…”

Section: Collection Maintenance Of Strains and Rna Sequencingmentioning

confidence: 99%

“…The identification, maintenance, and selection of Bt susceptible and resistant specimens was performed as described in Pezenti et al [2021]. After selection procedures, resistant and susceptible third instar larvae (at 242nd generation with resistance ratio = 133) were fed for 24 h on a diet containing Bt strain HD73 [Pezenti et al, 2021]. For The number of times each motif was identified in the libraries is given in parentheses.…”

Section: Collection Maintenance Of Strains and Rna Sequencingmentioning

confidence: 99%

“…There is a lack of genetic analyses and cytogenetic studies on the species, which are restricted to the location of repetitive DNAs, for example clusters of 18S rDNA [Magalhães et al, 2020]. Pezenti et al [2021], analyzing the transcriptional profile in resistant and susceptible A. gemmatalis strains bp, base pairs; SSR, simple sequence repeat; SUS, susceptible larvae fed conventional diet; SUSBt, susceptible larvae fed diet containing Bacillus thuringiensis (bt) HD73; RES, resistant larvae fed conventional diet; RESBt, resistant larvae fed diet containing Bt HD73. to Bt toxins, showed transcriptional differences in genes related to membrane receptors, metabolic detoxification pathways, and immune response genes.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptional Analysis of Microsatellites in Velvetbean Caterpillar Anticarsia gemmatalis Hübner, 1818

Dias

Souza

Pezenti

et al. 2022

Cytogenet Genome Res

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Brazil is the largest producer of soybeans in the world. The vast extent of soybean plantations across the Brazilian territory exposes this crop to attack by several insects, including the velvetbean caterpillar, Anticarsia gemmatalis. One of the alternatives used to control this insect are the toxins produced by Bacillus thuringiensis (Bt). However, in some cases, resistance to these toxins has been reported in the laboratory. Despite the ecological and economic impact of the velvetbean caterpillar, there are few studies on the genetic structure of this species, especially with regard to microsatellites. In this paper, we carried out a comparative transcriptional analysis of microsatellites in resistant (RES) and susceptible (SUS) strains of A. gemmatalis challenged and not challenged with Bt toxins. According to the number of sequences analyzed in each group, a 7.9% simple sequence repeat (SSR) rate was identified for the SUS library, and 7.4% for SUSBt. For the RES group, this value was 8.5% and for the RESBt 7.7%. Most of the fragments found showed a shorter repeat pattern, located in mono- and trinucleotide motifs. Among the 128 types of SSR motifs, it was possible to notice a large amount of adenine and thymine in relation to guanine and cytosine, which was also seen in chromosomes after staining with base-specific fluorochromes DAPI/CMA3, highlighting DAPI-positive regions. Although the participation of microsatellites in the resistance mechanism of A. gemmatalis to Bt is not clear, the results obtained in this work contribute to a better understanding of the repetitive DNA found in transcribed regions of a non-model organism.

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Genomic–proteomic analysis of a novel Bacillus thuringiensis strain: toxicity against two lepidopteran pests, abundance of Cry1Ac5 toxin, and presence of InhA1 virulence factor

et al. 2023

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Transcriptional profiling analysis of susceptible and resistant strains of Anticarsia gemmatalis and their response to Bacillus thuringiensis

Cited by 8 publications

References 55 publications

Involvement of an Enhanced Immunity Mechanism in the Resistance to Bacillus thuringiensis in Lepidopteran Pests

Involvement of an Enhanced Immunity Mechanism in the Resistance to Bacillus thuringiensis in Lepidopteran Pests

Transcriptional Analysis of Microsatellites in Velvetbean Caterpillar Anticarsia gemmatalis Hübner, 1818

Genomic–proteomic analysis of a novel Bacillus thuringiensis strain: toxicity against two lepidopteran pests, abundance of Cry1Ac5 toxin, and presence of InhA1 virulence factor

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