Transcriptional Profiles of Long Non-coding RNA and mRNA in Sheep Mammary Gland During Lactation Period

Chen, Wei-Hao; Lv, Xiaoyang; Wang, Yue; Zhang, Xinjun; Wang, Shanhe; Hussain, Zahid; Chen, Ling; Su, Rui; Sun, Wei

doi:10.3389/fgene.2020.00946

Cited by 8 publications

(9 citation statements)

References 69 publications

(68 reference statements)

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“…Collagen, as the most abundant protein in the body, is essential for maintaining the natural structure and strength of connective tissue(Chen et al 2020 ). Glycine, proline, and hydroxyproline are the main amino acids that make up the collagen structure.…”

Section: Discussionmentioning

confidence: 99%

“…COL3A1 was found to be DEG in this study. This evidence suggests that COL3A1 may play an important role in the sheep mammary epithelial cells cycle and can be considered as a candidate hub gene for future research in cows (Chen et al 2020 ). A study evaluating various miRNAs expressed in dairy heifers and dairy cows showed that COL1A2 is one of the genes associated with the cytoskeleton and extracellular matrix and is associated with mammary gland functional activity (Wicik et al 2016 ).…”

Section: Discussionmentioning

confidence: 99%

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Identification of Key Genes and Potential Pathways Associated with Mastitis Induced by E. coli

et al. 2022

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Mastitis is one of the most important diseases of dairy cattle. It is an infectious disease leading to an inflammatory reaction in the cow's mammary gland. Escherichia coli is one of the common bacteria which induce mastitis in cows. The aim of this study was to identify key genes and potential pathways associated with mastitis induced by E. coli in dairy cattle using bioinformatics analysis. The gene expression profile of ten samples including five adjacent tissues from a quarter infected with Escherichia coli and five tissues from a healthy quarter of dairy cattle was assessed using GEO2R. Gene ontology and pathway analysis were performed using bioinformatics tools. A total of 156 differentially expressed genes were detected which 95 genes were upregulated and 61 genes were downregulated in adjacent tissue of quarter infected compared with healthy tissue. Cellular oxidant detoxification and oxidation–reduction process were the most significant biological process terms in gene ontology analysis. The most important pathways of DEGs were the biosynthesis of amino acids, p53 signaling pathway, and Metabolic pathways. Three important modules were identified and their path enrichment analysis was performed. There are 10 core genes, among which SOD2, COL1A2, COL3A1, POSTN, ALDH18A1 , and CBS may be the main genes associated with mastitis, which can be considered as candidate genes in the prevention and carly diagnosis program of mastitis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Identification of Key Genes and Potential Pathways Associated with Mastitis Induced by E. coli

et al. 2022

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“…There is a growing number of reports on their involvement in host cell response (proliferation, differentiation, and apoptosis) to bacterial infections [73], as they are involved in epigenetic regulation, chromatin organization, transcriptional control, and pre-and posttranslational mRNA processing [93,94]. In addition, there have been reports about a novel mode of action of lncRNAs as competitive endogenous RNAs (ceRNAs), which affect the expression of mRNAs [95].…”

Section: Other Non-coding Rnasmentioning

confidence: 99%

Potential Novel Biomarkers for Mastitis Diagnosis in Sheep

Libera

Konieczny

Grabska

et al. 2021

Animals

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This review aims to characterize promising novel markers of ovine mastitis. Mastitis is considered as one of the primary factors for premature culling in dairy sheep and has noticeable financial, productional, and animal welfare-related implications. Furthermore, clinical, and subclinical mammary infections negatively affect milk yield and alter the milk composition, thereby leading to lowered quality of dairy products. It is, therefore, crucial to control and prevent mastitis through proper diagnosis, treatment or culling, and appropriate udder health management particularly at the end of the lactation period. The clinical form of mastitis is characterized by abnormalities in milk and mammary gland tissue alteration or systemic symptoms consequently causing minor diagnostic difficulties. However, to identify ewes with subclinical mastitis, laboratory diagnostics is crucial. Mastitis control is primarily dependent on determining somatic cell count (SCC) and the California Mastitis Test (CMT), which aim to detect the quantity of cells in the milk sample. The other useful diagnostic tool is microbial culture, which complements SCC and CMT. However, all mentioned diagnostic methods have their limitations and therefore novel biomarkers of ovine subclinical mastitis are highly desired. These sensitive indicators include acute-phase proteins, miRNA, and cathelicidins measurements, which could be determined in ovine serum and/or milk and in the future may become useful in early mastitis diagnostics as well as a preventive tool. This may contribute to increased detection of ovine mammary gland inflammation in sheep, especially in subclinical form, and consequently improves milk quality and quantity.

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“…In recent years, with the development of high-throughput sequencing, novel bioinformatics methods and related experimental validation, several classes of non-coding RNAs, such as circRNA, lncRNA and miRNA, have emerged and appear to play an important role in various biological processes (Chang et al, 2018;He et al, 2019;Chen et al, 2020;Zhou et al, 2020;Mei et al, 2021;Wu et al, 2021). CircRNA is different from linear RNA without 5 cap and 3 end poly-A tail.…”

Section: Introductionmentioning

confidence: 99%

Identification of Circular RNAs in the Ovaries of Hu Sheep and Local Fat-tailed Sheep during the Luteal Phase

Zhai¹,

Li²,

Zhang³

et al. 2022

IJAR

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Background: Circular RNAs (circRNAs), a novel class of endogenous non-coding RNAs with covalently closed loop structures, play important role in mammalian growth and development by regulating gene expression and participating in various biological processes. The aim of this study was to identify the expression profiles of circRNAs in the ovaries of Hu sheep and local Fat-tailed sheep during the luteal phase to determine the molecular mechanisms underlying the effects of circRNAs on reproduction and follicular development in sheep. Methods: To identify the differentially expressed circRNAs expressed in luteal phase conditions in Hu sheep and fat-tailed sheep, ovarian samples collected during the luteal phase were analyzed using high-throughput sequencing and subsequent transcriptomic approaches and bioinformatics analysis. Result: A total of 2,875 differentially expressed circRNAs were identified by high-through sequencing. Gene Ontology terminology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis showed that the host genes were mainly involved in the secretion and metabolism of the various pathway. Quantitative real-time PCR was performed to validate the results of expression profiling. These results contributed to an improved understanding of circRNA and the biological mechanisms of ovarian reproduction in sheep.

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Transcriptional Profiles of Long Non-coding RNA and mRNA in Sheep Mammary Gland During Lactation Period

Cited by 8 publications

References 69 publications

Identification of Key Genes and Potential Pathways Associated with Mastitis Induced by E. coli

Identification of Key Genes and Potential Pathways Associated with Mastitis Induced by E. coli

Potential Novel Biomarkers for Mastitis Diagnosis in Sheep

Identification of Circular RNAs in the Ovaries of Hu Sheep and Local Fat-tailed Sheep during the Luteal Phase

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