Transcriptional profile in rat muscle: down-regulation networks in acute strenuous exercise

Felipe, Stela Mirla da Silva; Freitas, Raquel Martins de; Penha, Emanuel Diego dos Santos; Pacheco, Christina; Martins, Daniel Freitas Freire; Alves, Juliana Osório; Soares, Paula Matias; Loureiro, Adriano César Carneiro; Lima, Tanes I.; Silveira, Leonardo R.; Ferraz, Alex Soares Marreiros; Souza, Jorge Estefano Santana de; Leal-Cardoso, José Henrique; Carvalho, Denise Pires de; Ceccatto, Vânia Marilande

doi:10.7717/peerj.10500

Cited by 2 publications

(6 citation statements)

References 49 publications

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“…A total of 12498 genes were found in RNAseq study results wherefrom the predicted target genes 1060 were present, while 142 were not found expressed in any of the conditions [19]. This finding demonstrates that 88.18% of the predicted targets genes are expressed in the study, which agrees with the down-regulation pattern of the miRNAs, since their decreased expression disables its potential action in the post-transcriptional silencing mechanism releasing the targets to express without its regulation interference.…”

Section: Target Genes Expressed In the Studysupporting

confidence: 65%

“…All exercise protocol, RNAseq experiment, and bioinformatic differential expression evaluation steps were performed as detailed described in the "Transcriptional profile in rat muscle: downregulation networks in acute strenuous exercise" [19], which is the main study of the project that the present article composes.…”

Section: Methodsmentioning

confidence: 99%

“…The experiment consisted of animal physical training, through submission to strenuous physical exercise in an adapted treadmill as previously described [19]. The animals choose for the study were healthy wistar male rats, 2months-old, with average weight of 240-280 g provided by the Superior Institute of Biomedical Sciences bioterium at the State University of Ceará, where were kept in cycles of light/dark (12h/12h), in a controlled temperature (22-25•C) environment, with water and feed ad libitum.…”

Section: Exercise Protocolmentioning

confidence: 99%

“…All RNAseq steps were performed as described in the "Transcriptional profile in rat muscle: downregulation networks in acute strenuous exercise" [19], main study of the project that the present article composed.…”

Section: Experimental Analysismentioning

confidence: 99%

“…The differentially expressed genes identified in the RNAseq results with EBseq package were compared to the 1201 target predicted gene from mir145 and mir186, which resulted in the finding of six corresponding genes (Table 3) between them. All of these genes presented a downregulation profile in strenuous exercise experiments considering the values of logFc through EBseq statistics evaluation [19].…”

Section: Target Genes Expressed In the Studymentioning

confidence: 99%

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mir186 and mir145 In vivo Evaluation and Enrichment in Rats Submitted to Treadmill Strenuous Exercise

Freitas

Pacheco

Felipe

et al. 2021

IJBcRR

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Aims: The present study aimed to identify miRNAs differentially expressed in rats submitted to strenuous exercise and in silico investigation of the biological implication of the findings. Place and Duration of Study: The in vivo experiments and analyses were performed in the Laboratory of Biochemistry and Gene Expression – LABIEX of the Superior Institute of Biomedical Science – ISCB from the State University of Ceará. Between 2017-2020. Methodology: The study was performed using as subjects 2-month-old male wistar rats, which initially were submitted to 2-week adaptation training. Later the animals were separated in two distinct groups, control (C) and trained (T), where only T performed a single session of strenuous exercise, while C were not submitted to this treatment. The applied exercise protocol consisted in a running training in treadmill with speed constant increasing until the animal exhaustion which was measured by the animal refusal to keep running. After 24h, soleus muscle was desiccated and submitted to RNAseq sequencing protocols. Obtained data were statistically evaluated in R environment with EBSeq package, to characterize and predict the miRNAs and their targets were used bioinformatics tools Gene Cards, mi RBase enrichR and KEGG. Results: Two differentially expressed miRNAs were found, mir145 and mir 186, both with downregulated expression pattern in strenuous exercise. These miRNAs have a total of 1201 predicted target genes, 67 were repeated and mostly correlate to cardiovascular disease pathways, between those 5 were differentially expressed as down-regulated. Conclusion: In conclusion, the findings suggest that mir186 and mir145 down-regulation profile mediated by strenuous exercise implicates in the non-alteration of the target genes expression profile, and consequently did not mediate alterations in the pathways they are evolved, which are mainly related to signaling and disorders.

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Section: Target Genes Expressed In the Studysupporting

confidence: 65%

Section: Methodsmentioning

confidence: 99%

Section: Exercise Protocolmentioning

confidence: 99%

Section: Experimental Analysismentioning

confidence: 99%

Section: Target Genes Expressed In the Studymentioning

confidence: 99%

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mir186 and mir145 In vivo Evaluation and Enrichment in Rats Submitted to Treadmill Strenuous Exercise

Freitas

Pacheco

Felipe

et al. 2021

IJBcRR

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Ferroptosis Contributed Genes in the Soleus Muscle After Endurance Training in Older Rats

Sahraee,

Salehpour,

Ashabi

2023

Preprint

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The main aim of the research is to investigate the effect of endurance physical activity on ChaC Glutathione Specific Gamma-Glutamyl cyclotransferase 1 (CHAC1), Glutamate-Cysteine Ligase Catalytic subunit (GCLC), Arachidonate 12-Lipoxygenase (ALOX12), as some ferroptosis related-genes expressed in soleus muscle tissue, in order to clarify apart of muscle atrophy physiology in old rats. Twenty old Wistar male rats were randomly assigned into two groups, endurance training and control groups. The training group ran incrementally on a treadmill, five days a week (velocity: 10–28 m/min). The expression genes were investigated by Real-time PCR and Malondialdehyde (MDA) and Superoxide dismutase (SOD) level as oxidative stress predicators were measured by assay kit. The mRNA expression of CHAC1 in the training group (0.36 ± 0.06)-fold decreased as opposed to the control group (P < 0.01). Increment in mRNA levels of GCLC and ALOX12 were seen in exercise group comparing with control, respectively (1.29 ± 0.21)-fold, (P > 0.01) and (1.84 ± 0.25)-fold, (P < 0.01). The level of MDA in the training group decreased significantly (1.18 ± 0.24) compared with the control group (1.58 ± 0.32), (P < 0.01). SOD level were higher in training (17.03 ± 2.10) compared with control (1.593 ± 14.45), (P < 0.01). It seems that regular aerobic running exercise could possibly Influence rodents muscle regulation through ferroptosis process and lipid peroxidation accumulation.

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Transcriptional profile in rat muscle: down-regulation networks in acute strenuous exercise

Cited by 2 publications

References 49 publications

mir186 and mir145 In vivo Evaluation and Enrichment in Rats Submitted to Treadmill Strenuous Exercise

mir186 and mir145 In vivo Evaluation and Enrichment in Rats Submitted to Treadmill Strenuous Exercise

Ferroptosis Contributed Genes in the Soleus Muscle After Endurance Training in Older Rats

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