Transcriptional pausing factor M1BP regulates cellular homeostasis by suppressing autophagy and apoptosis in
            <i>Drosophila</i>
            eye

Chimata, Anuradha Venkatakrishnan; Darnell, Hannah; Raj, Akanksha; Kango-Singh, Madhuri; Singh, Amit

doi:10.1080/27694127.2023.2252307

Cited by 1 publication

(1 citation statement)

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“…This is unsurprising, given that most peaks associated with cell cycle genes are localized to promoter regions. Insect transcription factor motifs that are enriched include: M1BP, a zinc finger factor which binds the Motif 1 promoter sequence and has been implicated in transcriptional pausing, insulator functions, cellular metabolism and homeostasis (Bag et al, 2021; Chimata et al, 2023; Li and Gilmour, 2013; Poliacikova et al, 2023); DREF, a BED-finger factor that is known to regulate proliferation and other developmental processes (Killip and Grewal, 2012; Tue et al, 2017); Trl (GAGA factor), a pioneer factor thought to regulate nucleosomal as well as higher-order chromatin organization (Chetverina et al, 2021; Gaskill et al, 2021; Li et al, 2023); and an E-box motif, previously found to be upstream of Drosophila core promoters and similar to the binding motif for Myc, a well-described cell cycle regulator (FitzGerald et al, 2006; Qi et al, 2022). Notably, analysis of vertebrate motifs revealed enrichment for multiple annotated E2F motifs.…”

Section: Resultsmentioning

confidence: 99%

Transcriptional repression and enhancer decommissioning silence cell cycle genes in postmitotic tissues

Fogarty,

Buchert,

et al. 2024

Preprint

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The mechanisms that maintain a non-cycling status in postmitotic tissues are not well understood. Many cell cycle genes have promoters and enhancers that remain accessible even when cells are terminally differentiated and in a non-cycling state, suggesting their repression must be maintained long term. In contrast, enhancer decommissioning has been observed for rate-limiting cell cycle genes in the Drosophila wing, a tissue where the cells die soon after eclosion, but it has been unclear if this also occurs in other contexts of terminal differentiation. In this study, we show that enhancer decommissioning also occurs at specific, rate-limiting cell cycle genes in the long-lived tissues of the Drosophila eye and brain, and we propose this loss of chromatin accessibility may help maintain a robust postmitotic state. We examined the decommissioned enhancers at specific rate-limiting cell cycle genes and show that they encode dynamic temporal and spatial expression patterns that include shared, as well as tissue-specific elements, resulting in broad gene expression with developmentally controlled temporal regulation. We extend our analysis to cell cycle gene expression and chromatin accessibility in the mammalian retina using a published dataset, and find that the principles of cell cycle gene regulation identified in terminally differentiating Drosophila tissues are conserved in the differentiating mammalian retina. We propose a robust, non-cycling status is maintained in long-lived postmitotic tissues through a combination of stable repression at most cell cycle gens, alongside enhancer decommissioning at specific rate-limiting cell cycle genes.

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