Transcriptional networks regulating suberin and lignin in endodermis link development and ABA response

Xu, Huimin; Liu, Peng; Wang, Chunhua; Wu, Shasha; Dong, Chen; Lin, Qingyun; Sun, Wenru; Huang, Ben-Ben; Xu, Meizhi; Tauqeer, Arfa; Wu, Shuang

doi:10.1093/plphys/kiac298

Cited by 29 publications

(25 citation statements)

References 56 publications

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“…Several copies of the well-known MYB4 phenylpropanoid biosynthesis regulator displayed an enhanced expression under MdMYB68 overexpression reaching 130.29-fold increase in expression ( Jin et al., 2000 ; Andersen et al., 2021 ). Finally, a MYB84-like transcription factor, which have been recently associated with the root suberization, is also highly induced ( Xu et al., 2022a ).…”

Section: Resultsmentioning

confidence: 99%

“…Seven days after infiltration, Nicotiana benthamiana leaf samples were collected and washed with phosphate buffer twice for 10 min, then dehydrated in a graded series of ethanol and resin embedded as described previously ( Xu et al., 2022a ). For immuno‐localization, sections (10µm) of tobacco leaves were processed as described in ( Behr et al., 2016 ).…”

Section: Methodsmentioning

confidence: 99%

“…Interestingly, a recent study proposed for the first time, a model aiming to explain an upstream/downstream regulation pathway for suberin biosynthesis, describing tier levels and TF-TF interactions ( Xu et al., 2022a ). This model highlighted that the Arabidopsis orthologous gene of MdMYB93 is in the downstream part of the regulatory cascade, whereas a AtMYB68 seemed to be involved in the upstream tier.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of MdMYB68, a suberin master regulator in russeted apples

Guerriero

Domergue

et al. 2023

Front. Plant Sci.

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IntroductionApple russeting is mainly due to the accumulation of suberin in the cell wall in response to defects and damages in the cuticle layer. Over the last decades, massive efforts have been done to better understand the complex interplay between pathways involved in the suberization process in model plants. However, the regulation mechanisms which orchestrate this complex process are still under investigation. Our previous studies highlighted a number of transcription factor candidates from the Myeloblastosis (MYB) transcription factor family which might regulate suberization in russeted or suberized apple fruit skin. Among these, we identified MdMYB68, which was co-expressed with number of well-known key suberin biosynthesis genes.MethodTo validate the MdMYB68 function, we conducted an heterologous transient expression in Nicotiana benthamiana combined with whole gene expression profiling analysis (RNA-Seq), quantification of lipids and cell wall monosaccharides, and microscopy.ResultsMdMYB68 overexpression is able to trigger the expression of the whole suberin biosynthesis pathway. The lipid content analysis confirmed that MdMYB68 regulates the deposition of suberin in cell walls. Furthermore, we also investigated the alteration of the non-lipid cell wall components and showed that MdMYB68 triggers a massive modification of hemicelluloses and pectins. These results were finally supported by the microscopy.DiscussionOnce again, we demonstrated that the heterologous transient expression in N. benthamiana coupled with RNA-seq is a powerful and efficient tool to investigate the function of suberin related transcription factors. Here, we suggest MdMYB68 as a new regulator of the aliphatic and aromatic suberin deposition in apple fruit, and further describe, for the first time, rearrangements occurring in the carbohydrate cell wall matrix, preparing this suberin deposition.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of MdMYB68, a suberin master regulator in russeted apples

Guerriero

Domergue

et al. 2023

Front. Plant Sci.

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“…The relative expression of eight genes related to apoplastic barriers in roots were investigated by qRT-PCR. Among them, four each that were respectively associated with CS formation and suberin monomer synthesis comprised MYB36, SHR1 (also regulates root suberization), PER64, and CASP (Naseer et al, 2012;Hosmani et al, 2013;Kamiya et al, 2015;Wang et al, 2020b;Wang et al, 2022a;Xu et al, 2022), and MYB41, CYP86A1, KCS20, and FAR1 (Höfer et al, 2008;Lee et al, 2009;Domergue et al, 2010;Kosma et al, 2014;Shukla et al, 2021). Osmotic stress increased the expression of PER64 in DS (24 h), downregulated that of MYB36, SHR1, and CASP to varying degrees in DS, and only slightly affected that of the four genes related to CS formation in DT (Figures 7A-D).…”

Section: Expression Of Genes Associated With Apoplastic Barriersmentioning

confidence: 99%

Endodermal apoplastic barriers are linked to osmotic tolerance in meso-xerophytic grass Elymus sibiricus

Liu

Wang²,

Yong-ping³

et al. 2022

Front. Plant Sci.

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Drought is the most serious adversity faced by agriculture and animal husbandry industries. One strategy that plants use to adapt to water deficits is modifying the root growth and architecture. Root endodermis has cell walls reinforced with apoplastic barriers formed by the Casparian strip (CS) and suberin lamellae (SL) deposits, regulates radial nutrient transport and protects the vascular cylinder from abiotic threats. Elymus sibiricus is an economically important meso-xerophytic forage grass, characterized by high nutritional quality and strong environmental adaptability. The purpose of this study was to evaluate the drought tolerance of E. sibiricus genotypes and investigate the root structural adaptation mechanism of drought-tolerant genotypes’ responding to drought. Specifically, a drought tolerant (DT) and drought sensitive (DS) genotype were screened out from 52 E. sibiricus genotypes. DT showed less apoplastic bypass flow of water and solutes than DS under control conditions, as determined with a hydraulic conductivity measurement system and an apoplastic fluorescent tracer, specifically PTS trisodium-8-hydroxy-1,3,6-pyrenetrisulphonic acid (PTS). In addition, DT accumulated less Na, Mg, Mn, and Zn and more Ni, Cu, and Al than DS, regardless of osmotic stress. Further study showed more suberin deposition in DT than in DS, which could be induced by osmotic stress in both. Accordingly, the CS and SL were deposited closer to the root tip in DT than in DS. However, osmotic stress induced their deposition closer to the root tips in DS, while likely increasing the thickness of the CS and SL in DT. The stronger and earlier formation of endodermal barriers may determine the radial transport pathways of water and solutes, and contribute to balance growth and drought response in E. sibiricus. These results could help us better understand how altered endodermal apoplastic barriers in roots regulate water and mineral nutrient transport in plants that have adapted to drought environments. Moreover, the current findings will aid in improving future breeding programs to develop drought-tolerant grass or crop cultivars.

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“…Interestingly, these mechanisms do not exist independently. A recent study from the perspective of root development in response to abiotic stress revealed that MYB36 balances root lignification and suberization by mediating the MYB74-MYB36-MYB92-MYB93 subnetwork ( Xu et al, 2022 ). The above studies indicate that the functions of MYB transcription factors are diversified, gradually forming a complex network mechanism.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide analysis of R2R3-MYB transcription factors in Boehmeria nivea (L.) gaudich revealed potential cadmium tolerance and anthocyanin biosynthesis genes

et al. 2023

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Gene family, especially MYB as one of the largest transcription factor family in plants, the study of its subfunctional characteristics is a key step in the study of plant gene function. The sequencing of ramie genome provides a good opportunity to study the organization and evolutionary characters of the ramie MYB gene at the whole genome level. In this study, a total of 105 BnGR2R3-MYB genes were identified from ramie genome and subsequently grouped into 35 subfamilies according to phylogeny divergence and sequences similarity. Chromosomal localization, gene structure, synteny analysis, gene duplication, promoter analysis, molecular characteristics and subcellular localization were accomplished using several bioinformatics tools. Collinearity analysis showed that the segmental and tandem duplication events is the dominant form of the gene family expansion, and duplications prominent in distal telomeric regions. Highest syntenic relationship was obtained between BnGR2R3-MYB genes and that of Apocynum venetum (88). Furthermore, transcriptomic data and phylogenetic analysis revealed that BnGMYB60, BnGMYB79/80 and BnGMYB70 might inhibit the biosynthesis of anthocyanins, and UPLC-QTOF-MS data further supported the results. qPCR and phylogenetic analysis revealed that the six genes (BnGMYB9, BnGMYB10, BnGMYB12, BnGMYB28, BnGMYB41, and BnGMYB78) were cadmium stress responsive genes. Especially, the expression of BnGMYB10/12/41 in roots, stems and leaves all increased more than 10-fold after cadmium stress, and in addition they may interact with key genes regulating flavonoid biosynthesis. Thus, a potential link between cadmium stress response and flavonoid synthesis was identified through protein interaction network analysis. The study thus provided significant information into MYB regulatory genes in ramie and may serve as a foundation for genetic enhancement and increased productivity.

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Transcriptional networks regulating suberin and lignin in endodermis link development and ABA response

Cited by 29 publications

References 56 publications

Characterization of MdMYB68, a suberin master regulator in russeted apples

Characterization of MdMYB68, a suberin master regulator in russeted apples

Endodermal apoplastic barriers are linked to osmotic tolerance in meso-xerophytic grass Elymus sibiricus

Genome-wide analysis of R2R3-MYB transcription factors in Boehmeria nivea (L.) gaudich revealed potential cadmium tolerance and anthocyanin biosynthesis genes

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