Transcriptional Decoding of Morphogen Gradients during Development

Garcia, Gloria R.; Larson, Daniel R.

doi:10.1016/j.devcel.2020.09.007

Cited by 2 publications

(2 citation statements)

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“…It has been shown for transcription of gap genes in Drosophila nc13 embryos that the initiation rate is constant for the different gap genes tested and at all positions across the expression domains. 42 While k on and k off have been reported to be regulated in response to differing concentrations of transcription factors and cell signals 45,46 , examples where Pol II initiation rate is modulated are rarer. One example is during the refinement of the seven even-skipped stripes in the Drosophila embryo.…”

Section: Discussionmentioning

confidence: 99%

Modulation of transcription burst amplitude underpins dosage compensation in theDrosophilaembryo

Beadle¹,

Zhou²,

Rattray³

et al. 2023

Preprint

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Dosage compensation, the balancing of X linked gene expression between sexes and to the autosomes, is critical to an organism's fitness and survival. In Drosophila, dosage compensation involves hypertranscription of the male X chromosome. Here we use quantitative live imaging and modelling at single-cell resolution to determine the mechanism underlying X chromosome dosage compensation in Drosophila. We show that the four X chromosome genes studied undergo transcriptional bursting in male and female embryos. Mechanistically our data reveal that transcriptional upregulation of male X chromosome genes is primarily mediated by a higher RNA polymerase II initiation rate and burst amplitude across the expression domain. In contrast, burst frequency is spatially modulated in nuclei within the expression domain in response to different transcription factor concentrations to tune the transcriptional response. Together, these data show how the local and global regulation of distinct burst parameters establish complex the transcriptional outputs underpinning developmental patterning.

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Section: Discussionmentioning

confidence: 99%

Modulation of transcription burst amplitude underpins dosage compensation in theDrosophilaembryo

Beadle¹,

Zhou²,

Rattray³

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…It has been shown for transcription of gap genes in Drosophila nc13 embryos that the initiation rate is constant for the different gap genes tested and at all positions across the expression domains. 42 While k on and k off have been reported to be regulated in response to differing concentrations of many transcription factors and cell signals, 45 , 46 examples where the RNA Pol II initiation rate is modulated are rarer. One example is during the refinement of the seven even-skipped stripes in the Drosophila embryo.…”

Section: Discussionmentioning

confidence: 99%