Transcriptional control of the Saccharomyces cerevisiae PGK gene by RAP1.

Chambers, A.; Tsang, Jwh; Stanway, Clive; Kingsman, A.J.; Kingsman, Susan M.

doi:10.1128/mcb.9.12.5516

Cited by 121 publications

(101 citation statements)

References 39 publications

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“…3) similar to that seen in the RAP1 binding site in the yeast PGK promoter (Fig. lc) [13]. Analysis of the coding strand detected no additional DNA/protein contacts between the rRGD sequence and the recombinant yeast transcription factor (Fig.…”

Section: Binding Of In Vitro Produced Rap1 Protein To the Rat Gastrinmentioning

confidence: 52%

“…2). Lysates programmed with RAP1 SP6 transcript formed a slowly migrating specific [38], PGK [13], HMRE, HMLE [22], hGRD [20], rGRD. The consensus RAP1 binding site is given in bold.…”

Section: Binding Of In Vitro Produced Rap1 Protein To the Rat Gastrinmentioning

confidence: 99%

“…The RAP1 binding site at the HMR silent mating-type locus is required for full repression of transcription [6][7][8][9][10]. RAPI binding sites are also found upstream of a large number of genes including ribosomal and glycolytic enzyme genes activating gene transcription [11][12][13][14]. RAP1 also binds to ARSs (autonomously replicating sequences) and telomeres of yeast chromosomes to regulate cell division [10,15].…”

Section: Introductionmentioning

confidence: 99%

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Activation of gastrin gene transcription in islet cells by a RAP1‐like cis‐acting promoter element

1994

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Gastrin transcription in islet cells is activated by a cis-regulatory sequence containing a binding site for the yeast transcription factor RAP1. The DNA-protein interactions between RAP1 protein and the gastrin DNA element determined by methylation interference assays are identical to those of RAP1 and yeast genes. Point mutations in the gastrin RAP1 binding site, which abolished RAP1 binding, decreased transcriptional activation by this sequence. Islet cells revealed a DNA binding protein with RAPl-like binding specificity. These findings support the conclusion that gastrin transcription is activated in mammalian cells by a RAPl-like transcription factor.

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Section: Binding Of In Vitro Produced Rap1 Protein To the Rat Gastrinmentioning

confidence: 52%

“…2). Lysates programmed with RAP1 SP6 transcript formed a slowly migrating specific [38], PGK [13], HMRE, HMLE [22], hGRD [20], rGRD. The consensus RAP1 binding site is given in bold.…”

Section: Binding Of In Vitro Produced Rap1 Protein To the Rat Gastrinmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Activation of gastrin gene transcription in islet cells by a RAP1‐like cis‐acting promoter element

1994

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“…Rap1p binds a divergent sequence (RMACCCAN-NCAYY) found in glycolytic gene promoters (47), whereas Gcr1p has been shown to bind a pentimer CTTCC (48). In the PGK promoter, one copy of the Rap1p recognition site coupled with three repeats of the Gcr1p binding motif are required for normal glucose induction (49). A similar organization is found in the UME1 promoter.…”

Section: Discussionmentioning

confidence: 78%

Ume1p Represses Meiotic Gene Transcription in Saccharomyces cerevisiae through Interaction with the Histone Deacetylase Rpd3p

Mallory

Strich

2003

Journal of Biological Chemistry

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Ume1p is a member of a conserved protein family including RbAp48 that associates with histone deacetylases. Consistent with this finding, Ume1p is required for the full repression of a subset of meiotic genes during vegetative growth in budding yeast. In addition to mitotic cell division, this report describes a new role for Ume1p in meiotic gene repression in precommitment sporulating cultures returning to vegetative growth. However, Ume1p is not required to re-establish repression as part of the meiotic transient transcription program. Mutational analysis revealed that two conserved domains (NEE box and a WD repeat motif) are required for Ume1p-dependent repression. Co-immunoprecipitation studies revealed that both the NEE box and the WD repeat motif are essential for normal Rpd3p binding. Finally, Ume1p-Rpd3p association is dependent on the global co-repressor Sin3p. Moreover, this activity was localized to one of the four paired amphipathic-helix domains of Sin3p shown previously to be required for transcriptional repression. These findings support a model that Ume1p binding to Rpd3p is required for its repression activity. In addition, these results suggest that Rpd3-Ume1p-Sin3p comprises an interdependent complex required for mediating transcriptional repression.

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“…Such sequences have also been described in the FBP1 and PCK1 promoters [32]. It should be noted that the GRF1 binding site operates in many cases together with a CTTCC motif [35] which is also present at positions -707, -435 and -347 in the APE1 promoter. The motif situated far upstream in the APE1 promoter might play at best a marginal role, since its removal causes only a slight decrease in fl-galactosidase expression in glucose-grown cells (compare the first two constructions of Fig.…”

Section: Regulatory Regions In the Ape1 Promotermentioning

confidence: 99%

Transcriptional regulation of the yeast vacuolar aminopeptidase yscI encoding gene (APE1) by carbon sources

1995

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Transcriptional control of the Saccharomyces cerevisiae PGK gene by RAP1.

Cited by 121 publications

References 39 publications

Activation of gastrin gene transcription in islet cells by a RAP1‐like cis‐acting promoter element

Activation of gastrin gene transcription in islet cells by a RAP1‐like cis‐acting promoter element

Ume1p Represses Meiotic Gene Transcription in Saccharomyces cerevisiae through Interaction with the Histone Deacetylase Rpd3p

Transcriptional regulation of the yeast vacuolar aminopeptidase yscI encoding gene (APE1) by carbon sources

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