Transcriptional Control of the Plant Cell Cycle

Doerner, Peter

doi:10.1007/7089_2007_120

Cited by 4 publications

(3 citation statements)

References 66 publications

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“…This inhibition is compatible with a change in the nuclear chromatin pattern, detected by chromatin staining using DAPI fluorescence, involving an increment of chromatin condensation. Thus, our results clearly support the association of chromatin remodelling with variations in transcriptional activity (Fedorova & Zink, 2008;Wang, Maharana, Wang, & Shivashankar, 2014 In highly proliferating cells, such as those of an in vitro suspension culture or meristematic tissues of plants, cell growth is strictly correlated to the production of proteins in order to overcome a biomass threshold compatible with cell division (Doerner, 2008;Mizukami, 2001). Consequently, cell growth is largely determined by the activity of biogenesis of ribosomes, the protein factories, which occurs in a nuclear domain, the nucleolus (Baserga, 2007;Bernstein et al, 2007;Bernstein & Baserga, 2004).…”

Section: Cell Cycle Progression Rate Is Increased Under Simulated Msupporting

confidence: 77%

“…In highly proliferating cells, such as those of an in vitro suspension culture or meristematic tissues of plants, cell growth is strictly correlated to the production of proteins in order to overcome a biomass threshold compatible with cell division (Doerner, ; Mizukami, ). Consequently, cell growth is largely determined by the activity of biogenesis of ribosomes, the protein factories, which occurs in a nuclear domain, the nucleolus (Baserga, ; Bernstein et al, ; Bernstein & Baserga, ).…”

Section: Discussionmentioning

confidence: 99%

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Cell cycle acceleration and changes in essential nuclear functions induced by simulated microgravity in a synchronized Arabidopsis cell culture

Kamal

Herranz

Loon

et al. 2018

Plant Cell & Environment

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Zero gravity is an environmental challenge unknown to organisms throughout evolution on Earth. Nevertheless, plants are sensitive to altered gravity, as exemplified by changes in meristematic cell proliferation and growth. We found that synchronized Arabidopsis-cultured cells exposed to simulated microgravity showed a shortened cell cycle, caused by a shorter G2/M phase and a slightly longer G1 phase. The analysis of selected marker genes and proteins by quantitative polymerase chain reaction and flow cytometry in synchronic G1 and G2 subpopulations indicated changes in gene expression of core cell cycle regulators and chromatin-modifying factors, confirming that microgravity induced misregulation of G2/M and G1/S checkpoints and chromatin remodelling. Changes in chromatin-based regulation included higher DNA methylation and lower histone acetylation, increased chromatin condensation, and overall depletion of nuclear transcription. Estimation of ribosome biogenesis rate using nucleolar parameters and selected nucleolar genes and proteins indicated reduced nucleolar activity under simulated microgravity, especially at G2/M. These results expand our knowledge of how meristematic cells are affected by real and simulated microgravity. Counteracting this cellular stress is necessary for plant culture in space exploration.

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Section: Cell Cycle Progression Rate Is Increased Under Simulated Msupporting

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Cell cycle acceleration and changes in essential nuclear functions induced by simulated microgravity in a synchronized Arabidopsis cell culture

Kamal

Herranz

Loon

et al. 2018

Plant Cell & Environment

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show abstract

“…Therefore, in highly proliferating cells, such as those of an in vitro suspension culture or meristematic tissues of plants, cell growth involves the production of proteins in order to overcome a biomass threshold (or cell size threshold) compatible with the viable size and biomass of daughter cells after mitosis (Doerner 2008). This is the reason for the need of a strict coordination between the rates of cell growth and cell division in proliferating cells, which defines meristematic competence (Mizukami 2001).…”

Section: B Effects Of Microgravity On Ribosome Biogenesis and The Mor...mentioning

confidence: 99%

Plants in Space: Novel Physiological Challenges and Adaptation Mechanisms

et al. 2021

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Any space exploration initiative, such as the human presence in the Moon and Mars, must incorporate plants for life support. To enable space plant culture we need to understand how plants respond to extraterrestrial conditions, adapt to them and compensate their deleterious effects at multiple levels. Gravity is a major difference between the terrestrial and the extraterrestrial environment. Gravitropism is the process of establishing a growth direction for plant organs according to the gravity vector. Gravity signals are sensed at specialized tissues by the motion of amyloplasts called statoliths and transduced to produce a cellular polarization capable of influencing the transport of auxin. Gravity alterations eventually result in changes in the lateral balance of auxin in the root, producing deviations of the growth direction. Under microgravity, auxin changes affect the root meristem causing increased cell proliferation and decreased cell growth. The nucleolus, the nuclear site of production of ribosomes, is a marker of this unbalance, which could alter plant development. At the molecular level, microgravity induces a reprogramming of gene expression that mostly affects plant defense systems against abiotic stresses, indicating that these categories of genes are involved in the adaptation to extraterrestrial habitats. Nevertheless, no specific genes for plant response to gravitational stress have been identified. Despite this stress, plants survive, developing until the adult stage and reproducing under microgravity conditions. A major research challenge is to identify environmental factors, such as light, which could interact, modulate or balance the impact of gravity, contributing to the tolerance and survival of plants under spaceflight conditions. Understanding the crosstalk between light and gravity sensing will contribute to the success of the next generation agriculture in human settlements outside the Earth. Plants are needed for space exploration: Space Plant Biology."Je ne sais pas si les mondes sont habités, et, comme je ne le sais pas, je vais y voir!" ("I do not know if the worlds are inhabited, and, as I do not know that, I'll go there and see!") -Jules Verne, "From the Earth to the Moon" (1867).This quote, from one of the most famous books by Jules Verne, which was written more than a century and a half ago, reflects an intense human dream, which, at the same time is a major challenge for the humankind: to go out of our planet Earth and see how are "the other worlds", in particular, whether we, as living beings and, especially, as intelligent living beings, are alone in the Universe, or we have companions with whom we can interact. Science-fiction literature is full of stories talking about the relationships between humans and aliens, whether they are peaceful or hostile.In 2019, the entire world has commemorated the 50 th anniversary of the first human footprint on the surface of the Moon, and in 2020, the twenty years of the continuous presence of humans as crew members of the International S...

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2010

Plant Cell Biology

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Transcriptional Control of the Plant Cell Cycle

Cited by 4 publications

References 66 publications

Cell cycle acceleration and changes in essential nuclear functions induced by simulated microgravity in a synchronized Arabidopsis cell culture

Cell cycle acceleration and changes in essential nuclear functions induced by simulated microgravity in a synchronized Arabidopsis cell culture

Plants in Space: Novel Physiological Challenges and Adaptation Mechanisms

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