Transcriptional control of flavonoid biosynthesis

Li, Shutian

doi:10.4161/psb.27522

Cited by 268 publications

(168 citation statements)

References 63 publications

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“…The expression of OsMYC2 is induced by JA treatment23. Several MYB proteins activate flavonoid biosynthetic genes by interacting with bHLH TFs24. Thus, we focused on the MYB and bHLH proteins among the selected TFs.…”

Section: Resultsmentioning

confidence: 99%

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OsMYC2, an essential factor for JA-inductive sakuranetin production in rice, interacts with MYC2-like proteins that enhance its transactivation ability

Ogawa

Miyamoto

Nemoto

et al. 2017

Sci Rep

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Biosynthesis of sakuranetin, a flavonoid anti-fungal phytoalexin that occurs in rice, is highly dependent on jasmonic acid (JA) signalling and induced by a variety of environmental stimuli. We previously identified OsNOMT, which encodes naringenin 7-O-methyltransferase (NOMT); NOMT is a key enzyme for sakuranetin production. Although OsNOMT expression is induced by JA treatment, the regulation mechanism that activates the biosynthetic pathway of sakuranetin has not yet been elucidated. In this study, we show that JA-inducible basic helix-loop-helix transcriptional factor OsMYC2 drastically enhances the activity of the OsNOMT promoter and is essential for JA-inducible sakuranetin production. In addition, we identified 2 collaborators of OsMYC2, OsMYC2-like protein 1 and 2 (OsMYL1 and OsMYL2) that further activated the OsNOMT promoter in synergy with OsMYC2. Physical interaction of OsMYC2 with OsMYL1 and OsMYL2 further supported the idea that these interactions lead to the enhancement of the transactivation activity of OsMYC2. Our results indicate that JA signalling via OsMYC2 is reinforced by OsMYL1 and OsMYL2, resulting in the inductive production of sakuranetin during defence responses in rice.

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Section: Resultsmentioning

confidence: 99%

“…The interaction of bHLH and MYB TFs often enhances the transcription of flavonoid biosynthetic genes24. To test this point, we next performed transient expression assays with dual effectors.…”

Section: Resultsmentioning

confidence: 99%

OsMYC2, an essential factor for JA-inductive sakuranetin production in rice, interacts with MYC2-like proteins that enhance its transactivation ability

Ogawa

Miyamoto

Nemoto

et al. 2017

Sci Rep

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“…In addition to their role in pigmentation in plant tissues, anthocyanins often accumulate in plant leaves, providing protection from stress conditions such as high light, ultraviolet (UV) light, cold temperature, or pathogen attack (Li et al 1993;Middleton and Teramura 1993;Dixon and Paiva 1995;Chalker-Scott and Scott 2004). Anthocyanins also have potential beneficial effects on human health and play key roles in the defense response as antimicrobial agents (Winkel-Shirley 2001;Li 2014).…”

Section: Introductionmentioning

confidence: 99%

“…The biosynthesis of anthocyanin is regulated by transcription factors such as HY5, R2R3-MYB and the MYB-bHLH-WD40 (MBW) complex, which control the expression of early or late biosynthetic genes (Chattopadhyay et al 1998;Koes et al 2005;Maier et al 2013;Shin et al 2013;Li 2014). MYB-related transcription factor genes that regulate flavonoid biosynthesis and metabolism have been isolated from many species, including Arabidopsis, tobacco, apple, grape and loquat (Espley et al 2007;Matus et al 2009;Dubos et al 2010;Liu et al 2013;Medina-Puche et al 2014;Xu et al 2014b).…”

Section: Introductionmentioning

confidence: 99%

Photoperiod and shading regulate coloration and anthocyanin accumulation in the leaves of malus crabapples

Zhang

Meng

et al. 2015

Plant Cell Tiss Organ Cult

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In many ornamental plants, anthocyanin accumulation in leaves is a critical determinant of foliage coloration. However, the mechanism by which photoperiod and shading affect coloration and anthocyanin accumulation in the leaves of woody plants is less clear. By examining the leaves and calli of crabapple (Malus spp.) cultivars with different leaf color characteristics, we analyzed the foliage coloration, flavonoid contents, and expression levels of anthocyanin biosynthetic genes and the McMYB10 transcription factor under different photoperiods and shading treatments. The red color parameters, flavonoid contents, and expression levels of some anthocyaninrelated genes in both the leaf and callus were generally higher under sun-exposure or long-day treatments compared to shading or short-day treatments. These data indicate that red color formation in cultivars with leaves that are naturally red requires a suitable photoperiod and light intensity; high light intensity or long-day conditions activate anthocyanin biosynthesis-related genes, leading to anthocyanin accumulation. Our results also indicate that the regulation of anthocyanin biosynthetic metabolism in response to light conditions differs between red-leafed and green-leafed cultivars. Taken together, our data reveal that photoperiod and light intensity are important in the regulation of pigmentation and anthocyanin accumulation in crabapple leaves and calli. Moreover, we provide a useful approach for studying and inducing the production of flavonoids by crabapple callus culture under certain photoperiods.

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“…In Arabidopsis , JAs can affect anthocyanin accumulation via the interaction of negative regulators with the MBW complex of transcription factors involved in anthocyanin biosynthesis [50]. Preharvest application of JA to “ Fuji ” apples enhances red coloration [51].…”

Section: Resultsmentioning

confidence: 99%

Comparative Transcriptome Analysis Reveals Effects of Exogenous Hematin on Anthocyanin Biosynthesis during Strawberry Fruit Ripening

Wang

et al. 2016

International Journal of Genomics

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Anthocyanin in strawberries has a positive effect on fruit coloration. In this study, the role of exogenous hematin on anthocyanin biosynthesis was investigated. Our result showed that the white stage of strawberries treated with exogenous hematin had higher anthocyanin content, compared to the control group. Among all treatments, 5 μM of hematin was the optimal condition to promote color development. In order to explore the molecular mechanism of fruit coloring regulated by hematin, transcriptomes in the hematin- and non-hematin-treated fruit were analyzed. A large number of differentially expressed genes (DEGs) were identified in regulating anthocyanin synthesis, including the DEGs involved in anthocyanin biosynthesis, hormone signaling transduction, phytochrome signaling, starch and sucrose degradation, and transcriptional pathways. These regulatory networks may play an important role in regulating the color process of strawberries treated with hematin. In summary, exogenous hematin could promote fruit coloring by increasing anthocyanin content in the white stage of strawberries. Furthermore, transcriptome analysis suggests that hematin-promoted fruit coloring occurs through multiple related metabolic pathways, which provides valuable information for regulating fruit color via anthocyanin biosynthesis in strawberries.

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Transcriptional control of flavonoid biosynthesis

Cited by 268 publications

References 63 publications

OsMYC2, an essential factor for JA-inductive sakuranetin production in rice, interacts with MYC2-like proteins that enhance its transactivation ability

OsMYC2, an essential factor for JA-inductive sakuranetin production in rice, interacts with MYC2-like proteins that enhance its transactivation ability

Photoperiod and shading regulate coloration and anthocyanin accumulation in the leaves of malus crabapples

Comparative Transcriptome Analysis Reveals Effects of Exogenous Hematin on Anthocyanin Biosynthesis during Strawberry Fruit Ripening

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