Transcriptional changes in pupal hypoderm in Drosophila melanogaster

Mitchell, Herschel K.; Lipps, Loveriza S.; Tracy, Ursula M.

doi:10.1007/bf00520199

Cited by 33 publications

(6 citation statements)

References 12 publications

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“…Variant tRNA-induced mistranslation affects a wide range of physiological processes and exerts both positive and negative effects on flies. Mistranslation is most deleterious during periods of intense growth and translational activity, including embryogenesis and pupation (Mitchell et al 1977;Mitchell and Petersen 1981;Trumbly and Jarry 1983;Qin et al 2007).…”

Section: Implications For Multicellular Eukaryotesmentioning

confidence: 99%

Mistranslating tRNA variants have anticodon- and sex-specific impacts onDrosophila melanogaster

Isaacson,

Berg,

Jagiello

et al. 2024

Preprint

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Transfer RNAs (tRNAs) are vital in determining the specificity of translation. Mutations in tRNA genes can result in the misincorporation of amino acids into nascent polypeptides in a process known as mistranslation. Since mistranslation has different impacts, depending on the type of amino acid substitution, our goal here was to compare the impact of different mistranslating tRNAServariants on fly development, lifespan, and behaviour. We established two mistranslating fly lines, one with a tRNAServariant that misincorporates serine at valine codons (V➔S) and the other that misincorporates serine at threonine codons (TàS). While both mistranslating tRNAs increased development time and developmental lethality, the severity of the impacts differed depending on amino acid substitution and sex. The V➔S variant extended embryonic, larval, and pupal development whereas the T➔S only extended larval and pupal development. Females, but not males, containing either mistranslating tRNA presented with significantly more anatomical deformities than controls. Mistranslating females also experienced extended lifespan whereas mistranslating male lifespan was unaffected. In addition, mistranslating flies from both sexes showed improved locomotion as they aged, suggesting delayed neurodegeneration. Therefore, although mistranslation causes detrimental effects, we demonstrate that mistranslation also has positive effects on complex traits such as lifespan and locomotion. This has important implications for human health given the prevalence of tRNA variants in humans.PLAIN LANGUAGE SUMMARYMutant tRNA genes can cause mistranslation, the misincorporation of amino acids into proteins, and are associated with several human diseases. This study investigated the role of two tRNA variants that cause threonine-to-serine (T➔S) or valine-to-serine (V➔S) substitution. Both variants caused developmental delays and lethality in both sexes and increase prevalence of deformities in females. Surprisingly, female T➔S and V➔S flies experienced increased lifespan and mistranslating males and females showed improved locomotion. These results suggest that mistranslation has both positive and negative effects that depend on the tRNA variant and sex of the fly.

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Section: Implications For Multicellular Eukaryotesmentioning

confidence: 99%

Mistranslating tRNA variants have anticodon- and sex-specific impacts onDrosophila melanogaster

Isaacson,

Berg,

Jagiello

et al. 2024

Preprint

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“…Due to the availability of a putative electrophoretic mutant of the KIL-1 protein, we could test for the presumption that prepupal salivary gland proteins contribute to the pupation fluid, which covers the external surface of the pupating animal (Mitchell et al, 1977b;Sarmiento and Mitchell, 1982). Samples of the wildtype pupation fluid were collected and the proteins analysed by SDS-PAGE.…”

Section: The Kil-1 Encoded Product Is Secreted Into the Pupation Fluidmentioning

confidence: 99%

“…The presence of predominant low molecular weight components among prepupal salivary gland proteins has been demonstrated in various Drosophila species (Mitchell et al, 1977b;Kress, 1982;Sarmiento and Mitchell, 1982;Shirk et al, 1988). Hence, they might be major representatives of prepupal salivary gland function.…”

Section: Introductionmentioning

confidence: 98%

A Kunitz type protease inhibitor related protein is synthesized in Drosophila prepupal salivary glands and released into the moulting fluid during pupation

Kress

Jarrin

Thüroff

et al. 2004

Insect Biochemistry and Molecular Biology

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“…He has identified a group of essential antigens ("housekeeping" proteins), most of which are present in all stages and tissues studied, and a group of "luxury" antigens, which varies considerably with developmental stage and tissue type. Striking differences in patterns of protein synthesis, examined by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, has been shown in salivary glands, thoracic epithelium, and other Drosophila tissues labeled with [35S]methionine at various times during development [14][15][16]. Rodgers and Shearn [17], using two-dimensional gel electrophoresis, have shown that about 15 to 20% of the proteins synthesized in wing, leg, and eye-antenna1 imaginal disks are unique to either one disk type, or to specific pairs of disks.…”

Section: Introductionmentioning

confidence: 99%

Developmental modulation of protein synthesis in Drosophila primary embryonic cell cultures

Buzin

Seecof

1981

Dev. Genet.

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The patterns of proteins synthesized during embryonic development in Drosophila melanogaster have been examined by two-dimensional gel electrophoresis. Primary cell cultures prepared from donor embryos synchronized to & 1 hr were labeled with [35S]methionine at 5, 11.5, 14.5, and 26 hr after oviposition. Of approximately 400 to 500 proteins detected, the synthesis of about 50 is developmentally modulated. The greatest number of changes in the synthesis of stage-specific proteins occurs at 11.5 and 14.5 hr after oviposition, periods just prior to and during the times of the greatest overt morphological and biochemical changes. At 11.5 hr, 35 stage-specific proteins are synthesized, including 19 that are not present at the previous stage examined. At 14.5 hr, 34 stage-specific proteins can be detected, including 11 newly synthesized proteins. However, 12 proteins from the previous stage are no longer synthesized. At the completion of embryonic differentiation, at 26 hr, no new proteins are synthesized and the synthesis of many present in earlier stages has decreased or stopped. Comparison of patterns of embryonic proteins to those synthesized by two Drosophila continuous cell lines reveals that the majority of proteins are common to all. However, only about 40% of the embryonic stage-specific proteins are present in either cell line. In addition, there are several proteins unique to each cell line that are not observed in any of the embryonic stages.

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Transcriptional changes in pupal hypoderm in Drosophila melanogaster

Cited by 33 publications

References 12 publications

Mistranslating tRNA variants have anticodon- and sex-specific impacts onDrosophila melanogaster

Mistranslating tRNA variants have anticodon- and sex-specific impacts onDrosophila melanogaster

A Kunitz type protease inhibitor related protein is synthesized in Drosophila prepupal salivary glands and released into the moulting fluid during pupation

Developmental modulation of protein synthesis in Drosophila primary embryonic cell cultures

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