Transcriptional and proteomic analysis reveal recombinant galectins of Haemonchus contortus down-regulated functions of goat PBMC and modulation of several signaling cascades in vitro

Wang, Wang; Yuan, Cheng; Wang, Shuai; Song, Xiaoling; Xu, Lixin; Ren, Yan; Hasson, I.A.; Li, Xiangrui

doi:10.1016/j.jprot.2013.12.017

Cited by 40 publications

(48 citation statements)

References 79 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Proteomic analysis of excretory/secretory (ES) proteins indicated galectins were expressed numerously in H. contortus , Ostertagia ostertagi and Teladorsagia circumcincta [40]. Recently, we found that rHco-gal-m/f could be recognized by the antiserum from goats experimentally infected with H. contortus and interacted with goat PBMC in vitro [18]. All of these results indicated that galectins of H. contortus were excretory/secretory antigens and interacted with the host immune system during infection.…”

Section: Discussionmentioning

confidence: 99%

“…Rat polyclonal antibodies against rHco-gal-m were prepared as described previously [18]. Briefly, about 0.3 mg of the purified rHco-gal-m was mixed with Freund’s complete adjuvant of a 1:1 mixture and injected into SD rats subcutaneously in multiple places followed the method described by Han et al .…”

Section: Methodsmentioning

confidence: 99%

“…RNA extraction and preparation of cDNA were performed as previously reported [18]. In brief, total RNA was isolated from eggs, L3, xL3, L4 and adult worms using RNeasy Mini Kit (Qiagen, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…RNA extraction and preparation of cDNA were performed as previously reported [18]. Real-time PCR was conducted on the ABI 7500 Real-Time PCR System (Applied Biosystems, USA), using standard procedure (Stage 1: Initial denaturation, 95°C for 30 s, 1 cycle; Stage 2: Amplification, 95°C for 5 s, 60°C for 60 s, 40 cycles; Melt Curve Stage: 60°C–95°C, ramp rate 1%).…”

Section: Methodsmentioning

confidence: 99%

“…They inhibited the hemagglutination of goat erythrocytes [14], suppressed cytokine mRNA transcription [15], induced apoptosis of the goat PBMC [16], and showed partial immunoprotective effects against H. contortus infection [17]. Recently, a combined proteomic and transcriptomic analysis revealed that the activations of VEGF pathway, free radical producing pathway, NFκB pathway and ubiquitin–proteasome pathway in goat PBMC were down-regulated by rHco-gal-m/f [18]. …”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Galectin Hco-gal-m from Haemonchus contortus modulates goat monocytes and T cell function in different patterns

Wang

Zhang

et al. 2014

Parasites Vectors

Self Cite

View full text Add to dashboard Cite

BackgroundMonocytes and T cells are two major subpopulations of peripheral blood mononuclear cells (PBMC) and play an essential role in the innate and adaptive immune systems. Different members of the galectin family show multiple and distinct regulatory effects on different cell types. Previous studies have demonstrated that the galectin from Haemonchus contortus (Hco-gal-m) performed immunomodulatory effects on goat PBMC, however, which subpopulation of PBMC is the primary target of Hco-gal-m and whether the immune modulations share the same mechanism remain unclear.MethodsIn this study, the developmental expression of Hco-gal-m was analyzed by RT-PCR and Western blot analysis. The distribution of Hco-gal-m in adult worm was detected by an immunohistochemical test. The binding activity of the recombinant Hco-gal-m (rHco-gal-m) on goat monocytes and T cells were assessed by flow cytometry. The immunomodulatory effects of Hco-gal-m on cytokine secretion, cell activation and apoptosis were observed by co-incubation of rHco-gal-m with goat monocytes and T cells.ResultsHco-gal-m was expressed in L4 as well as adult worms and predominantly localized at the internal surface of the worm guts. rHco-gal-m could bind to both monocytes and T cells. The engagement of rHco-gal-m decreased the production of IL-6, IL-10 and TNF-α in T cells, however, it significantly increased the secretion of IL-10 in monocytes. After rHco-gal-m exposure, the expression of MHC-II on monocytes and that of CD25 on T cells were restricted. Consequently, T cell proliferations were potently inhibited by rHco-gal-m. In addition, rHco-gal-m induced apoptosis in T cells, but not significantly in monocytes.ConclusionsOur results indicated that rHco-gal-m modulated goat monocytes and T cell function in different patterns.Electronic supplementary materialThe online version of this article (doi:10.1186/1756-3305-7-342) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%