Transcriptional and Posttranscriptional Events Control Copper-Responsive Expression of a Rhodobacter capsulatus Multicopper Oxidase

Rademacher, Corinna; Moser, Roman; Lackmann, Jan-Wilm; Klinkert, Birgit; Narberhaus, Franz; Masepohl, Bernd

doi:10.1128/jb.06274-11

Cited by 20 publications

(28 citation statements)

References 56 publications

(47 reference statements)

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“…Most copper‐sensing regulators are intracytoplasmic and periplasmic transcriptional regulators (Inesi ), but it was discovered that a post‐transcriptional copper response mechanism also exists (Rademacher et al . ).…”

Section: Mechanisms Of Microbial Copper Resistancementioning

confidence: 97%

Contact killing and antimicrobial properties of copper

et al. 2018

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With the emergence of antibiotic resistance, the interest for antimicrobial agents has recently increased again in public health. Copper was recognized in 2008 by the United States Environmental Protection Agency (EPA) as the first metallic antimicrobial agent. This led to many investigations of the various properties of copper as an antibacterial, antifungal and antiviral agent. This review summarizes the latest findings about 'contact killing', the mechanism of action of copper nanoparticles and the different ways micro-organisms develop resistance to copper.

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Section: Mechanisms Of Microbial Copper Resistancementioning

confidence: 97%

Contact killing and antimicrobial properties of copper

et al. 2018

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“…To determine whether the synergistic effect of Cu(II) and Fe(II) was unique to R. palustris, we tested the effects of Cu(II) and Fe(II) on E. coli MG1655, Shewanella oneidensis MR-1, and Rhodobacter capsulatus SB1003. The physiology of these organisms has been studied extensively, and their copper resistance systems have been at least partially characterized (12,(32)(33)(34). In testing the sensitivities of these organisms to copper and iron, we chose various copper and iron concentrations to reflect the various sensitivities to copper of the different bacterial taxa.…”

Section: Synergistic Effect Of Cu(ii) and Fe(ii)mentioning

confidence: 99%

Iron and Copper Act Synergistically To Delay Anaerobic Growth of Bacteria

Bird

Coleman

Newman

2013

Appl Environ Microbiol

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bTransition metals are known to cause toxic effects through their interaction with oxygen, but toxicity under anoxic conditions is poorly understood. Here we investigated the effects of iron (Fe) and copper (Cu) on the anaerobic growth and gene expression of the purple phototrophic bacterium Rhodopseudomonas palustris TIE-1. We found that Fe(II) and Cu(II) act synergistically to delay anaerobic growth at environmentally relevant metal concentrations. Cu(I) and Cu(II) had similar effects both alone and in the presence of ascorbate, a Cu(II) reductant, indicating that reduction of Cu(II) to Cu(I) by Fe(II) is not sufficient to explain the growth inhibition. Addition of Cu(II) increased the toxicity of Co(II) and Ni(II); in contrast, Ni(II) toxicity was diminished in the presence of Fe(II). The synergistic anaerobic toxicity of Fe(II) and Cu(II) was also observed for Escherichia coli MG1655, Shewanella oneidensis MR-1, and Rhodobacter capsulatus SB1003. Gene expression analyses for R. palustris identified three regulatory genes that respond to Cu(II) and not to Fe(II): homologs of cueR and cusR, two known proteobacterial copper homeostasis regulators, and csoR, a copper regulator recently identified in Mycobacterium tuberculosis. Two P-type ATPase efflux pumps, along with an F o F 1 ATP synthase, were also upregulated by Cu(II) but not by Fe(II). An Escherichia coli mutant deficient in copA, cus, and cueO showed a smaller synergistic effect, indicating that iron might interfere with one or more of the copper homeostasis systems. Our results suggest that interactive effects of transition metals on microbial physiology may be widespread under anoxic conditions, although the molecular mechanisms remain to be more fully elucidated.

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“…The MerR-like Cu sensor CueR binds Cu(I) via two conserved cysteine residues in the C-terminal MBD [ 185 ]. The apo-CueR interacts with DNA and prevents RNA polymerase from binding [ 293 , 294 , 295 ]. Upon Cu(I) binding, CueR undergoes a conformational change and up-regulates the transcription of copA, cueO and copZ via a conserved DNA-distortion mechanism [ 293 , 294 , 295 ].…”

Section: Copper Export Across the Cytoplasmic Membranementioning

confidence: 99%

“…The apo-CueR interacts with DNA and prevents RNA polymerase from binding [ 293 , 294 , 295 ]. Upon Cu(I) binding, CueR undergoes a conformational change and up-regulates the transcription of copA, cueO and copZ via a conserved DNA-distortion mechanism [ 293 , 294 , 295 ]. The CueR levels are furthermore controlled by ATP-dependent proteolysis [ 296 ].…”

Section: Copper Export Across the Cytoplasmic Membranementioning

confidence: 99%

“…In R. capsulatus , cutG ( rcc02109 ) is part of the cutFOG operon [ 254 ]. It was shown that the transcription of this operon strictly depends on the promoter located upstream of cutF and that the cutF - cutO intergenic region is essential for Cu dependent expression of cutO and cutG [ 293 , 294 ]. Comparative differential cuproproteome analyses of R. capsulatus revealed that the CutO and CutG protein levels are greatly increased in the presence of Cu [ 74 ].…”

Section: Periplasmic Copper Chaperones and Their Targetsmentioning

confidence: 99%

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Cu Homeostasis in Bacteria: The Ins and Outs

et al. 2020

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Copper (Cu) is an essential trace element for all living organisms and used as cofactor in key enzymes of important biological processes, such as aerobic respiration or superoxide dismutation. However, due to its toxicity, cells have developed elaborate mechanisms for Cu homeostasis, which balance Cu supply for cuproprotein biogenesis with the need to remove excess Cu. This review summarizes our current knowledge on bacterial Cu homeostasis with a focus on Gram-negative bacteria and describes the multiple strategies that bacteria use for uptake, storage and export of Cu. We furthermore describe general mechanistic principles that aid the bacterial response to toxic Cu concentrations and illustrate dedicated Cu relay systems that facilitate Cu delivery for cuproenzyme biogenesis. Progress in understanding how bacteria avoid Cu poisoning while maintaining a certain Cu quota for cell proliferation is of particular importance for microbial pathogens because Cu is utilized by the host immune system for attenuating pathogen survival in host cells.

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Transcriptional and Posttranscriptional Events Control Copper-Responsive Expression of a Rhodobacter capsulatus Multicopper Oxidase

Cited by 20 publications

References 56 publications

Contact killing and antimicrobial properties of copper

Contact killing and antimicrobial properties of copper

Iron and Copper Act Synergistically To Delay Anaerobic Growth of Bacteria

Cu Homeostasis in Bacteria: The Ins and Outs

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