Transcriptional and post‐translational regulation of neutral trehalase in <i>Schizosaccharomyces pombe</i> during thermal stress

Paredes, Vanessa; Franco, Alejandro; Madrid, Marisa; Soto, Teresa; Vicente-Soler, Jero; Gacto, Mariano; Cansado, José

doi:10.1002/yea.1128

Cited by 10 publications

(4 citation statements)

References 32 publications

(53 reference statements)

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“…Pap1 accumulates in the nucleus in a stress-and Spc1-dependent manner and is required for survival under low levels of oxidative stress (25,28,36). Although the precise mechanisms by which Spc1 activates Atf1 or Pap1 dependent transcription are unclear, a number of target stress response genes have now been identified which are dependent on Spc1, Atf1, or Pap1 (2,27,30,35,44,45).…”

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confidence: 99%

Stress-Activated Protein Kinase Pathway Functions To Support Protein Synthesis and Translational Adaptation in Response to Environmental Stress in Fission Yeast

et al. 2005

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The stress-activated protein kinase (SAPK) pathway plays a central role in coordinating gene expression in response to diverse environmental stress stimuli. We examined the role of this pathway in the translational response to stress in Schizosaccharomyces pombe. Exposing wild-type cells to osmotic stress (KCl) resulted in a rapid but transient reduction in protein synthesis. Protein synthesis was further reduced in mutants disrupting the SAPK pathway, including the mitogen-activated protein kinase Wis1 or the mitogen-activated protein kinase Spc1/Sty1, suggesting a role for these stress response factors in this translational control. Further polysome analyses revealed a role for Spc1 in supporting translation initiation during osmotic stress, and additionally in facilitating translational adaptation. Exposure to oxidative stress (H 2 O 2 ) resulted in a striking reduction in translation initiation in wild-type cells, which was further reduced in spc1 ؊ cells. Reduced translation initiation correlated with phosphorylation of the ␣ subunit of eukaryotic initiation factor 2 (eIF2␣) in wild-type cells. Disruption of Wis1 or Spc1 kinase or the downstream bZip transcription factors Atf1 and Pap1 resulted in a marked increase in eIF2␣ phosphorylation which was dependent on the eIF2␣ kinases Hri2 and Gcn2. These findings suggest a role for the SAPK pathway in supporting translation initiation and facilitating adaptation to environmental stress in part through reducing eIF2␣ phosphorylation in fission yeast.

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confidence: 99%

Stress-Activated Protein Kinase Pathway Functions To Support Protein Synthesis and Translational Adaptation in Response to Environmental Stress in Fission Yeast

et al. 2005

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“…Atf1 and/or Pcr1 are key responders/effectors of many other signals, including those induced by osmolarity, oxidative state, heavy metals, abundance of nutrients, carbon source, DNA damage and chronological aging (42,47,51,54,60,(64)(65)(66)(67)(68)(69)(70)(71)(72)(73)(74)(75)(76)(77)(78)(79)(80). These multiple, diverse signals-like those generated by differences in temperature-probably impinge upon Atf1 and Pcr1 to control rates of recombination at M26-class hotspots in the genome.…”

Section: A Model For Environmentally and Metabolically Induced Plastimentioning

confidence: 99%

Molecular mechanisms for environmentally induced plasticity in the positioning of meiotic recombination at hotspots

Mukiza

Protacio

Davidson

et al. 2020

Preprint

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In meiosis, Spo11/Rec12-initiated homologous recombination is clustered at hotspots that regulate its frequency and distribution across the genome. Intriguingly, the intensities and positions of recombination hotspots can change dramatically in response to intracellular and extracellular conditions, and can display epigenetic memory. Here, using the fission yeast Schizosaccharomyces pombe, we reveal mechanisms for hotspot plasticity. We show that each of six hotspot-activating proteins (transcription factors Atf1, Pcr1, Php2, Php3, Php5, Rst2) is rate-limiting for promoting recombination at its own DNA binding site, allowing each class of hotspot to be regulated independently by agonistic and antagonistic signals. We also discovered that the regulatory protein-DNA complexes can establish a recombinationally poised epigenetic state before meiosis. Notably, Atf1 and Pcr1 controlled the activation of DNA sequence-dependent hotspots to which they do not bind; and they do so by regulating the expression of other hotspot-activating proteins. Thus, while each transcription factor activates its own class of DNA sequence-dependent hotspots directly in cis, cross-talk between regulatory networks modulates in trans the frequency and positioning of recombination at other classes of DNA sequence-dependent hotspots. We posit that such mechanisms allow cells to alter the frequency distribution of meiotic recombination in response to metabolic states and environmental cues.

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“…Generally in yeast, the regulation of trehalose synthesis and breakdown is done by cAMP-dependent phosphorylation mechanisms ( 24 ). It has been reported that the ntp1 + expression is regulated by the pathway of protein kinase cascade activated under osmotic or oxidative stress ( 23 , 25 ) or by the binding of the transcription factors to elements such as cAMP-response element under thermal stress ( 26 ). In the present study, ird5 and ird11 were used to evaluate whether or not trehalose contributes to survival under moderate oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

Role of Oxidative Stress Response and Trehalose Accumulation in the Longevity of Fission Yeast

Palabıyık

Ghods

2015

Jundishapur J Microbiol

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Background:Glucose is the preferred carbon and energy source in most organisms and plays an active role in the regulation of many biological processes. However, an excess of glucose leads to such undesirable conditions as diabetes and age-related diseases. Since Schizosaccharomyces pombe homologous of many human genes, it offers several advantages for the investigation of the molecular mechanisms underlying human disease and aging studies. We have identified two glucose-repression-resistant mutants (ird5 and ird11) of S. pombe.Objectives:We aimed to investigate the possible relationship between lifespan extension and oxidative stress response induced by exposure to hydrogen peroxide alongside the trehalose accumulation level by using the two S. pombe mutants (i.e. ird5 and ird11), which are repressed by glucose and are resistant to oxidative stress.Materials and Methods:We employed trehalose accumulation measurement and colony-forming unit (CFU) counting using the ird mutants in exponential and stationary phases and compared them to the wild type grown in repressed, de-repressed, and stressed conditions to clarify the possible relationship between glucose signaling, oxidative stress response, and lifespan in S. pombe.Results:The lifespan of the ird5 mutant was significantly longer that of either the ird11 mutant or the wild type cells. Under repressed condition, the trehalose content was increased remarkably on the 3rd day of the study in the ird11 mutant and the wild type. Under de-repressed condition, the level of intracellular trehalose was notably increased on the 3rd day in ird11. Under stressed condition, the trehalose level in ird11 was increased on the 3rd day as a pattern similar to that observed in the wild type.Conclusions:Our results demonstrated no significant correlation between the ird5 lifespan and the trehalose concentration. Likewise, the correlation between lifespan extension, trehalose accumulation, and cellular resistance to hydrogen peroxide was not significant.

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Transcriptional and post‐translational regulation of neutral trehalase in Schizosaccharomyces pombe during thermal stress

Cited by 10 publications

References 32 publications

Stress-Activated Protein Kinase Pathway Functions To Support Protein Synthesis and Translational Adaptation in Response to Environmental Stress in Fission Yeast

Stress-Activated Protein Kinase Pathway Functions To Support Protein Synthesis and Translational Adaptation in Response to Environmental Stress in Fission Yeast

Molecular mechanisms for environmentally induced plasticity in the positioning of meiotic recombination at hotspots

Role of Oxidative Stress Response and Trehalose Accumulation in the Longevity of Fission Yeast

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