Transcriptional analysis of the cell division-related ssg genes in Streptomyces coelicolor reveals direct control of ssgR by AtrA

Kim, Songhee H.; Traag, Bjørn A.; Hasan, Ayad H.; McDowall, Kenneth J.; Kim, Byung‐Gee; Wezel, Gilles P. van

doi:10.1007/s10482-015-0479-2

Cited by 17 publications

(19 citation statements)

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“…There is also growing evidence that besides DasR, the TetR6family regulator AtrA plays a role in carbon utilization ( (Fig. 4) 206 , the transcriptional activator of the gene encoding SsgA, which is involved in cell division and sporulation 207,208 Interestingly, there is an intricate link between Rok7B7, DasR and CCR, which in turn has important implications for the control of antibiotic production. Proteomic comparison of S. coelicolor and a glkA null mutant showed that glucose activates the expression of Rok7B7 in a Glk6independent manner 214 , which was later confirmed by transcriptomic analysis 215 .…”

Section: Competition Between Atra Rok7b7 and Dasr And Connections Tomentioning

confidence: 99%

“…. Disruption of atrA suggests it activates transcription of ssgR206 , and direct binding of AtrA within the upstream regulatory region of ssgR was confirmed by ChIP6seq (McDowall et al, unpubl. data).The ROK6family protein, Rok7B7 takes up an interesting position in the regulatory network as it connects the control of antibiotic production and carbon catabolite repression209 .…”

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confidence: 93%

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Regulation of antibiotic production in Actinobacteria: new perspectives from the post-genomic era

et al. 2018

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Covering: 2000 to 2018 The antimicrobial activity of many of their natural products has brought prominence to the Streptomycetaceae, a family of Gram-positive bacteria that inhabit both soil and aquatic sediments. In the natural environment, antimicrobial compounds are likely to limit the growth of competitors, thereby offering a selective advantage to the producer, in particular when nutrients become limited and the developmental programme leading to spores commences. The study of the control of this secondary metabolism continues to offer insights into its integration with a complex lifecycle that takes multiple cues from the environment and primary metabolism. Such information can then be harnessed to devise laboratory screening conditions to discover compounds with new or improved clinical value. Here we provide an update of the review we published in NPR in 2011. Besides providing the essential background, we focus on recent developments in our understanding of the underlying regulatory networks, ecological triggers of natural product biosynthesis, contributions from comparative genomics and approaches to awaken the biosynthesis of otherwise silent or cryptic natural products. In addition, we highlight recent discoveries on the control of antibiotic production in other Actinobacteria, which have gained considerable attention since the start of the genomics revolution. New technologies that have the potential to produce a step change in our understanding of the regulation of secondary metabolism are also described.

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Section: Competition Between Atra Rok7b7 and Dasr And Connections Tomentioning

confidence: 99%

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confidence: 93%

Regulation of antibiotic production in Actinobacteria: new perspectives from the post-genomic era

et al. 2018

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“…Previous work revealed that the small protein SCO1839 is a candidate nucleoid-associated protein (NAP) (19), and it specifically binds to the promoter region of the sporulation regulatory gene ssgR (22). SsgR activates transcription of ssgA , which encodes a pleiotropic developmental regulator and activator of sporulation-specific cell division in streptomycetes.…”

Section: Resultsmentioning

confidence: 99%

Systems-wide analysis of the GATC-binding nucleoid-associated protein Gbn and its impact onStreptomycesdevelopment

Willemse

et al. 2021

Preprint

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Bacterial chromosome structure is organized by a diverse group of proteins collectively called nucleoid-associated proteins (NAPs). Many NAPs have been studied in detail in Streptomyces, including Lsr2, HupA, HupS, and sIHF. Here, we show that SCO1839 represents a novel family of small NAPs unique to Actinobacteria and recognizes a consensus sequence consisting of GATC followed by (A/T)T. The protein was designated Gbn for GATC-binding NAP. Chromatin immunoprecipitation sequencing (ChIP-Seq) detected more than 2800 binding regions, encompassing some 3600 GATCWT motifs, which comprise 55% of all motifs in the S. coelicolor genome. DNA binding of Gbn in vitro increased DNA stiffness but not compaction, suggesting a role in regulation rather than chromosome organization. Despite the huge number of binding sites, the DNA binding profiles were nearly identical during vegetative and aerial growth. The exceptions were SCO1311 and SCOt32, for a tRNA editing enzyme and a tRNA that recognises the rare leucine codon CUA, respectively, which were nearly exclusively bound during vegetative growth. Deletion of gbn led to pleiotropic alterations in developmental timing, morphogenesis and antibiotic production. Taken together, our data show that Gbn is a highly pleiotropic NAP that impacts growth and development in streptomycetes.

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“…Like DasR, the TetR‐family transcriptional regulator AtrA is highly conserved in streptomycetes, and AtrA is required for actinorhodin production in S. coelicolor and streptomycin production in Streptomyces griseus , by directly controlling the pathway‐specific activator genes actII ‐ORF4 and strR , respectively (Uguru et al ., ; Hong et al ., ). AtrA affects multiple regulatory pathways, including those that control sporulation (Nothaft et al ., ; Kim et al ., ). Interestingly, AtrA controls both the initial and final steps of the proposed DasR‐mediated signalling pathway, namely the internalization of the signal (GlcNAc) via the activation of the transporter gene nagE2 , and activation of the biosynthetic cluster for actinorhodin production via the transcriptional activation of actII‐ORF4 (Nothaft et al ., ).…”

Section: Cross‐talk Between Atra Dasr and Rok7b7mentioning

confidence: 97%

Intertwining nutrient‐sensory networks and the control of antibiotic production inStreptomyces

Urem

Świątek-Połatyńska

Rigali

et al. 2016

Molecular Microbiology

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Summary Actinobacteria are producers of a plethora of natural products of agricultural, biotechnological and clinical importance. In an era where mankind has to deal with rapidly spreading antimicrobial resistance, streptomycetes are of particular importance as producers of half of all antibiotics used in the clinic. Genome sequencing efforts revealed that their capacity as antibiotic producers has been underestimated, in particular as many biosynthetic pathways are silent under standard laboratory conditions. Here we review the global regulatory networks that control antibiotic production in streptomycetes, with emphasis on carbon‐ and aminosugar‐related nutrient sensory pathways. Recent research has revealed intriguing connections between these regulons, and overlap and antagonism between the activities of among others the global regulatory proteins AtrA, DasR and Rok7B7 as well as GlnR (nitrogen control) and PhoP (phosphate control), are discussed. Finally, we provide ideas as to how these novel insights might help us to find ways to activate the transcription of silent biosynthetic gene clusters.

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Transcriptional analysis of the cell division-related ssg genes in Streptomyces coelicolor reveals direct control of ssgR by AtrA

Cited by 17 publications

References 60 publications

Regulation of antibiotic production in Actinobacteria: new perspectives from the post-genomic era

Regulation of antibiotic production in Actinobacteria: new perspectives from the post-genomic era

Systems-wide analysis of the GATC-binding nucleoid-associated protein Gbn and its impact onStreptomycesdevelopment

Intertwining nutrient‐sensory networks and the control of antibiotic production inStreptomyces

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