Transcriptional analysis of prsA and vraTS regulatory system in methicillin resistant Staphylococcus aureus against oxacillin stress

Bhowmik, Deepshikha; Das, Bhaskar Jyoti; Hazarika, Monalisha; Chanda, Debadatta Dhar; Bhattacharjee, Amitabha

doi:10.1016/j.ijmmb.2021.10.009

Cited by 1 publication

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“…The Vra TSR system consists of Vra S (sensor histidine kinase), Vra R (response regulator), and Vra T for the stimulation and activation of Vra TSR. The vraS and vraR genes play a crucial role in the regulation of antibiotic resistance [ 40 ]. Activation of the three-component regulatory system VraTSR reduces the sensitivity of staphylococci to vancomycin, daptomycin, and oxacillin [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

Gausemycin A-Resistant Staphylococcus aureus Demonstrates Affected Cell Membrane and Cell Wall Homeostasis

2023

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Antibiotic resistance is a significant and pressing issue in the medical field, as numerous strains of infectious bacteria have become resistant to commonly prescribed antibiotics. Staphylococcus aureus is a bacterium that poses a grave threat, as it is responsible for a large number of nosocomial infections and has high mortality rates worldwide. Gausemycin A is a new lipoglycopeptide antibiotic that has considerable efficacy against multidrug-resistant S. aureus strains. Although the cellular targets of gausemycin A have been previously identified, detailing the molecular processes of action is still needed. We performed gene expression analysis to identify molecular mechanisms that may be involved in bacterial resistance to gausemycin A. In the present study, we observed that gausemycin A-resistant S. aureus in the late-exponential phase showed an increased expression of genes involved in cell wall turnover (sceD), membrane charge (dltA), phospholipid metabolism (pgsA), the two-component stress-response system (vraS), and the Clp proteolytic system (clpX). The increased expression of these genes implies that changes in the cell wall and cell membrane are essential for the bacterial resistance to gausemycin A. In the stationary phase, we observed a decrease in the expression of genes involved in the phospholipid metabolism (mprF) and Clp proteolytic system (clpX).

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Section: Discussionmentioning

confidence: 99%