Transcriptional analysis of flagellar and putative virulence genes of Arcobacter butzleri as an endocytobiont of Acanthamoeba castellanii

Medina, Gustavo; Neves, Patrícia; Flores-Martin, Sandra N.; Manosalva, Carolina; Andaur, Marcela; Otth, Carola; Lincopán, Nilton; Fernández, Heriberto

doi:10.1007/s00203-019-01678-0

Cited by 9 publications

(7 citation statements)

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“…Apart from intra-amoebic microorganisms, a wet-bench study investigated the ecological relation between amoeba and mosquitoes, demonstrating for the first time that A. polyphaga can infect Aedes aegypti. (94) Moreover, the role of FLA as vehicles of pathogenic microorganisms debated worldwide (95,96,97) and also present in Brazilian surveys (85,88,91,98) reinforce the need of monitoring FLA presence in the environment.…”

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confidence: 98%

“…Some reports discuss interactions of FLAs with fungi and bacteria, addressing both endosymbionts and pathogens of concern in human health. (19,(85)(86)(87)(88)(89)(90) Another type of ARMs studied in Brazil comprises human adenovirus (91) and giant viruses such as the Tupanvirus, isolated from V. vermiformis and A. polyphaga mimivirus. (92,93) We discussed below other reports on giant virus classified in the auxiliary class.…”

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confidence: 99%

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A history of over 40 years of potentially pathogenic free-living amoeba studies in Brazil - a systematic review

Bellini

Thiemann

Reyes-Batllé

et al. 2022

Mem. Inst. Oswaldo Cruz

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Free-living amoeba (FLA) group includes the potentially pathogenic genera Acanthamoeba, Naegleria, Balamuthia, Sappinia, and Vermamoeba, causative agents of human infections (encephalitis, keratitis, and disseminated diseases). In Brazil, the first report on pathogenic FLA was published in the 70s and showed meningoencephalitis caused by Naegleria spp. FLA studies are emerging, but no literature review is available to investigate this trend in Brazil critically. Thus, the present work aims to integrate and discuss these data. Scopus, PubMed, and Web of Science were searched, retrieving studies from 1974 to 2020. The screening process resulted in 178 papers, which were clustered into core and auxiliary classes and sorted into five categories: wet-bench studies, dry-bench studies, clinical reports, environmental identifications, and literature reviews. The papers dating from the last ten years account for 75% (134/178) of the total publications, indicating the FLA topic has gained Brazilian interest. Moreover, 81% (144/178) address Acanthamoeba-related matter, revealing this genus as the most prevalent in all categories. Brazil's Southeast, South, and Midwest geographic regions accounted for 96% (171/178) of the publications studied in the present work. To the best of our knowledge, this review is the pioneer in summarising the FLA research history in Brazil.

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confidence: 98%

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confidence: 99%

A history of over 40 years of potentially pathogenic free-living amoeba studies in Brazil - a systematic review

Bellini

Thiemann

Reyes-Batllé

et al. 2022

Mem. Inst. Oswaldo Cruz

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“…Previously, we described some of the mechanisms associated with the endosymbiotic process between A. butzleri and A. castellanii under controlled laboratory conditions (Fernández et al 2012;Medina et al 2014Medina et al , 2019Villanueva et al 2016). This bacterium acts as an endocytobiont of A. castellanii due to its ability to establish itself within amoebic vacuoles, surviving for at least 10 days in absence of bacterial replication.…”

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confidence: 99%

“…Trophozoites were maintained in log phase at 25 °C in T-25 tissue culture asks with 10 mL of modi ed peptone-yeast-glucose (PYG) medium. Prior to the assays a suspension of 5 × 10 6 amoebae/mL in PBS 1X was adjusted as previously described by Medina et al (2019). A. butzleri ATCC 49616 and native strain HF28, isolated from an environment water source, were used in all experiments.…”

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Long-term survive of Aliarcobacter butzleri in two models symbiotic interaction with Acanthamoeba castellanii

et al. 2022

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Aliarcobacter butzleri (formerly known as Arcobacter butzleri) is an emerging foodborne zoonotic pathogen that establishes in vitro endosymbiotic relationships with Acanthamoeba castellanii, a freeliving amoeba. Previously, we described that this bacterium acts as an endocytobiont of A. castellanii, surviving for at least 10 days in absence of bacterial replication. Thus, the aim of this study was to evaluate the ability of A. butzleri to survive as a long-term endosymbiont of A. castellanii for 30 days in two models of symbiotic interaction with A. castellanii: i) endosymbiotic culture followed by gentamicin protection assay and ii) transwell co-culture assay. The results allow us to conclude that A. butzleri is capable of surviving as an endosymbiont of A. castellanii for at least 30 days, without multiplying, under controlled laboratory conditions. In addition, in the absence of nutrients and as both microorganisms remain in the same culture, separated by semi-permeable membranes, A. castellanii does not promote the survival of A. butzleri, nor does it multiply. Our ndings suggest that the greater survival capacity of A.butzleri is associated with their endosymbiont status inside A. castellanii, pointing out the complexity of this type of symbiotic relationship. Full TextAcanthamoeba castellanii is a ubiquitous free-living amoeba (FLA) that plays an important role in the ecology of multiple ecosystems due to its participation in nutrient recycling, mainly in aqueous

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“…In figure 1.8, those reports grouped as in vitro comprise: gene silencing in Acanthamoeba (198); molecular detection of Acanthamoeba by showing randomly amplified polymorphic DNA (RAPD) as a suitable strategy to rapid genotyping new isolates; (196) metabolism studies in Naegleria (199)(200) and Acanthamoeba, (201)(202); interactions among FLA and intracellularly resistant microorganisms, (173,(203)(204)(205)(206); serach for antibodies as detection tool for Acanthamoeba infections, (207) and assays demonstrating amoeba capability of infecting mosquitoes, by using Acanthamoeba polyphaga and Aedes aegypti as studies of case. (208) In vitro assays by using natural substances as bacteriocin-like compounds (209) or synthetical substances as biguanides (e.g.…”

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confidence: 99%

Diversity of free-living amoebas in the Monjolinho River in São Paulo state – morphological and molecular approaches

Bellini¹

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I kindly thank my Brazilian advisor Prof. Otavio Henrique Thiemann, his knowledge and all advisements that contributed to the development of the present PhD study. I thanks for the many years of guidance that enriched my concept of making science and I thank the opportunity he gave to me of conducting the research in my ways. I deeply thank my Spanish advisor Prof. Jacob Lorenzo-Morales, whose support, suggestions, and expertise to guide my scientific steps were invaluable. I thanks for the enthusiasm to discuss science, for his availability to receive me at his laboratory, and all opportunities he provided to me enabling the enrichment of my PhD research. I sincerely thank both supervisors for having settled the cotutelle agreement, the origin of the present Double Degree PhD. I thank you both for all assistance, professional instructions, and encouragement along my PhD journey.The laboratory members: I thank all members of the Structural Biology Laboratory (USP). You all have helped in different manners, sharing the daily lab journey and motivating to go further. I thank you for each scientific discussion, exchange of knowledge, and opportunity to enhance scientific abilities. I thanks to Ivan, Marco Túlio, Tatiana, Evandro, Diego, Murilo, Vitor, Witer, and Fernanda. Special thanks go to Ana Letícia and Matheus Issa, undergraduate students who have been working with me. It has been an exciting challenge and a great opportunity to develop manners to teach scientific skills. I have also learned a lot with you both, thank you! I thank Dra. Susana Sculaccio, for her extensive support and technical expertise, shared with me during my scientific development. I also thank her for years of friendship. As well for Dr. Humberto, I thank his support in the laboratory routine. Also, a special thank to the Biophysics Laboratory at IFSC/USP assistance, in persons of Bel, Andressa, Rafael! My gratitude to all members of the Free Living Amoeba Laboratory

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Transcriptional analysis of flagellar and putative virulence genes of Arcobacter butzleri as an endocytobiont of Acanthamoeba castellanii

Cited by 9 publications

References 45 publications

A history of over 40 years of potentially pathogenic free-living amoeba studies in Brazil - a systematic review

A history of over 40 years of potentially pathogenic free-living amoeba studies in Brazil - a systematic review

Long-term survive of Aliarcobacter butzleri in two models symbiotic interaction with Acanthamoeba castellanii

Diversity of free-living amoebas in the Monjolinho River in São Paulo state – morphological and molecular approaches

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Transcriptional analysis of flagellar and putative virulence genes of Arcobacter butzleri as an endocytobiont of Acanthamoeba castellanii

Cited by 9 publications

References 45 publications

A history of over 40 years of potentially pathogenic free-living amoeba studies in Brazil - a systematic review

A history of over 40 years of potentially pathogenic free-living amoeba studies in Brazil - a systematic review

Long-term survive of Aliarcobacter butzleri in two models symbiotic interaction with Acanthamoeba castellanii

Diversity of free-living amoebas in the Monjolinho River in São Paulo state &ndash; morphological and molecular approaches

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Diversity of free-living amoebas in the Monjolinho River in São Paulo state – morphological and molecular approaches