Transcriptional Alteration of Gene Biomarkers in Hemocytes of Wild Ostrea edulis with Molecular Evidence of Infections with Bonamia spp. and/or Marteilia refringens Parasites

Cocci, Paolo; Roncarati, Alessandra; Capriotti, Martina; Mosconi, Gilberto; Palermo, Francesco Alessandro

doi:10.3390/pathogens9050323

Cited by 6 publications

(5 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Backg Rou N Dmentioning

confidence: 99%

“…Unlike the Crassostrea oysters Crassostrea gigas or Crassostrea virginica , O. edulis is a larviparous species with asynchronous hermaphroditism and rhythmic consecutive sexuality. Molecular studies on the physiology of the flat oyster, including basic traits and adaptative response to abiotic parameters, are rare and mainly focused on the study of the response of O. edulis to its parasite B. ostreae in laboratory experiments (Cocci et al, 2020 ; Gervais et al, 2019 ; Ronza et al, 2018 ). Several programs have aimed to identify quantitative trait loci (QTLs) associated with B. ostreae resistance with the use of comparative transcriptomic approaches (Pardo et al, 2016 ; Ronza et al, 2018 ) and SNP genotyping (Vera et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Chromosomal assembly of the flat oyster (Ostrea edulis L.) genome as a new genetic resource for aquaculture

Boutet

Monteiro

Baudry

et al. 2022

Evolutionary Applications

View full text Add to dashboard Cite

The European flat oyster (Ostrea edulis L.) is a native bivalve of the European coasts. Harvest of this species has declined during the last decades because of the appearance of two parasites that have led to the collapse of the stocks and the loss of the natural oyster beds. O. edulis has been the subject of numerous studies in population genetics and on the detection of the parasites Bonamia ostreae and Marteilia refringens. These studies investigated immune responses to these parasites at the molecular and cellular levels. Several genetic improvement programs have been initiated especially for parasite resistance. Within the framework of a European project (PERLE 2) that aims to produce genetic lines of O. edulis with hardiness traits (growth, survival, resistance) for the purpose of repopulating natural oyster beds in Brittany and reviving the culture of this species in the foreshore, obtaining a reference genome becomes essential as done recently in many bivalve species of aquaculture interest. Here, we present a chromosome‐level genome assembly and annotation for the European flat oyster, generated by combining PacBio, Illumina, 10X linked, and Hi‐C sequencing. The finished assembly is 887.2 Mb with a scaffold‐N50 of 97.1 Mb scaffolded on the expected 10 pseudochromosomes. Annotation of the genome revealed the presence of 35,962 protein‐coding genes. We analyzed in detail the transposable element (TE) diversity in the flat oyster genome, highlighted some specificities in tRNA and miRNA composition, and provided the first insight into the molecular response of O. edulis to M. refringens. This genome provides a reference for genomic studies on O. edulis to better understand its basic physiology and as a useful resource for genetic breeding in support of aquaculture and natural reef restoration.

show abstract

Section: Backg Rou N Dmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Chromosomal assembly of the flat oyster (Ostrea edulis L.) genome as a new genetic resource for aquaculture

Boutet

Monteiro

Baudry

et al. 2022

Evolutionary Applications

View full text Add to dashboard Cite

show abstract

“…virginica, O. edulis is a larviparous species with an asynchronous hermaphrodite with rhythmic consecutive sexuality. Molecular studies on the physiology of flat oyster, including basics traits and adaptative response to abiotic parameters, are rare and mainly focused on the study of the response of O. edulis to its parasite B. ostreae mainly in laboratory experiments (Ronza et al, 2018; Gervais et al, 2019; Cocci et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Chromosomal assembly of the flat oyster (Ostrea edulisL.) genome as a new genetic ressource for aquaculture

Boutet

Monteiro

Takeuchi

et al. 2022

Preprint

View full text Add to dashboard Cite

The European flat oyster (Ostrea edulis L.) is the endemic species of the European coasts. Its exploitation has been reduced during the last decades, because of the appearance of two parasites that have led to the collapse of the stocks and the strong decline of the natural oyster beds. O. edulis has been the subject of numerous studies and programs in population genetics and on the presence of the parasites Bonamia ostreae and Marteilia refringens. These studies investigated the effects of these parasites mainly on immunity at the molecular and cellular levels. Several genetic selection programs especially related to resistance to the parasite have been initiated. Within the framework of a European project (PERLE 2) which aims to produce genetic lines of O. edulis with hardiness traits (growth, survival, resistance) for the purpose of repopulating natural oyster beds in Brittany and reviving the culture of this species on the foreshore, obtaining a reference genome has proved to be essential as done recently in many bivalve species of aquaculture interest. Here, we present a chromosome-level genome assembly and annotation for the European flat oyster, generated by combining PacBio technology, Illumina, 10X linked and Hi-C sequencing. The finished assembly is 887.2 Mb with a scaffold-N50 of 97.1 Mb scaffolded on the expected 10 pseudo-chromosomes. Annotation of the genome revealed the presence of 35962 protein-coding genes. We analyzed in details the transposable elements (TE) diversity in the flat oyster genome, highlight some specificities in tRNA and miRNA composition and provide first insights in the molecular response of O. edulis to M. refringens. This genome will serve as a reference for genomic studies on O. edulis to better understand its basic physiology or developing genetic markers in breeding projects for aquaculture or natural reef restoration.

show abstract

“…Comparison between wild populations and oysters selected for resistance showed that inhibition of apoptosis, and an increase in IAP expression, predominates in the wild oysters (82). Thus, apoptosis of infected cells or haemocytes, which have phagocyted parasites, can prevent the spread of this pathogen (82,92). Gervais and colleagues (48,93) reported that apoptosis in haemocytes occurred in O. edulis 1-4 h post Bonamia contact, and continued to induce the expression of apoptotic genes many days after infection, but with differences between individuals originating from different locations in France.…”

Section: Caspases In Bivalve Development Immune System Function Andmentioning

confidence: 99%

Phylogenetic analysis of the caspase family in bivalves: implications for programmed cell death, immune response and development

Vogeler

Carboni

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Apoptosis is an important process for an organism’s innate immune system to respond to pathogens, while also allowing for cell differentiation and other essential life functions. Caspases are one of the key protease enzymes involved in the apoptotic process, however there is currently a very limited understanding of bivalve caspase diversity and functions. Results: In this work, we investigated the presence of caspase homologs using a combination of bioinformatics and phylogenetic analyses. We blasted the Crassostrea gigas genome for caspase homologs and identified thirty-five potential homologs in the addition to the already cloned twenty-three bivalve caspases. As such, we present information about the phylogenetic relationship of all identified bivalve caspases in relation to their homology to well-established vertebrate and invertebrate caspases. Our results reveal unexpected novelty and complexity in the bivalve caspase family. Notably, we were unable to identify direct homologues to the initiator caspase-9, a key-caspase in the vertebrate apoptotic pathway, inflammatory caspases (caspase-1,-4 or -5) or executioner caspases-3, -6, -7. We also explored the fact that bivalves appear to possess several unique homologs to the initiator caspase groups -2 and -8. Large expansions of caspase-3 like homologues (caspase-3A-C), caspase-3/7 group and caspase-3/7-like homologues were also identified, suggesting unusual roles of caspases with direct implications for our understanding of immune response in relation to common bivalve diseases. Furthermore, we assessed the gene expression of two initiator (Cg2A, Cg8B) and four executioner caspases (Cg3A, Cg3B, Cg3C, Cg3/7) in C. gigas late-larval development and during metamorphosis, indicating that caspase expressions vary across the different developmental stages.Conclusion: Our analysis provides the first overview of caspases across different bivalve species with essential new insight into caspase diversity, knowledge that can be used for further investigations into response to pathogens or regulation of developmental processes.

show abstract

Transcriptional Alteration of Gene Biomarkers in Hemocytes of Wild Ostrea edulis with Molecular Evidence of Infections with Bonamia spp. and/or Marteilia refringens Parasites

Cited by 6 publications

References 44 publications

Chromosomal assembly of the flat oyster (Ostrea edulis L.) genome as a new genetic resource for aquaculture

Chromosomal assembly of the flat oyster (Ostrea edulis L.) genome as a new genetic resource for aquaculture

Chromosomal assembly of the flat oyster (Ostrea edulisL.) genome as a new genetic ressource for aquaculture

Phylogenetic analysis of the caspase family in bivalves: implications for programmed cell death, immune response and development

Contact Info

Product

Resources

About